Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury
Background/Aims: Low-level laser therapy (LLLT) leads to complex photochemical responses during the healing process of spinal cord injury (SCI). Confocal Raman Microspectral Imaging (in combination with multivariate analysis) was adopted to illustrate the underlying biochemical mechanisms of LLLT tr...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Cell Physiol Biochem Press GmbH & Co KG
2018-09-01
|
Series: | Cellular Physiology and Biochemistry |
Subjects: | |
Online Access: | https://www.karger.com/Article/FullText/493295 |
id |
doaj-5bf72f89ae9b4c9dbc558c90d2dbafff |
---|---|
record_format |
Article |
spelling |
doaj-5bf72f89ae9b4c9dbc558c90d2dbafff2020-11-25T01:57:03ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-09-014931168118310.1159/000493295493295Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord InjuryYuze GongShuang WangZhuowen LiangZhe WangXu ZhangJie LiJiwei SongXueyu HuKaige WangQingli HeJintao BaiBackground/Aims: Low-level laser therapy (LLLT) leads to complex photochemical responses during the healing process of spinal cord injury (SCI). Confocal Raman Microspectral Imaging (in combination with multivariate analysis) was adopted to illustrate the underlying biochemical mechanisms of LLLT treatment on a SCI rat model. Methods: Using transversal tissue sections, the Raman spectra can identify areas neighboring the injury site, glial scar, cavity, and unharmed white matter, as well as their correlated cellular alterations, such as demyelination and up-regulation of chondroitin sulfate proteoglycans (CSPGs). Multivariate data analysis methods are used to depict the underlying therapeutic effects by highlighting the detailed content and distribution variations of the biochemical constituents. Results: It is confirmed that photon-tissue interactions might lead to a decay of the inhibitory response to remyelination by suppressing CSPG expression, as also morphologically demonstrated by reduced glial scar and cavity areas. An inter-group comparison semi-quantitatively confirms changes in lipids, phosphatidic acid, CSPGs, and cholesterol during SCI and its LLLT treatment, paving the way for in vitro and in vivo understanding of the biochemical changes accompanying pathobiological SCI events. Conclusion: The achieved results in this work not only have once again proved the well-known cellular mechanisms of SCI, but further illustrate the underlying biochemical variability during LLLT treatment, which provide a sound basis for developing real-time Raman methodologies to monitor the efficacy of the SCI LLLT treatment.https://www.karger.com/Article/FullText/493295Confocal RamanMicrospectral ImagingLow-level Laser TherapySpinal Cord InjuryPhotobiological EffectsPathobiological Analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuze Gong Shuang Wang Zhuowen Liang Zhe Wang Xu Zhang Jie Li Jiwei Song Xueyu Hu Kaige Wang Qingli He Jintao Bai |
spellingShingle |
Yuze Gong Shuang Wang Zhuowen Liang Zhe Wang Xu Zhang Jie Li Jiwei Song Xueyu Hu Kaige Wang Qingli He Jintao Bai Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury Cellular Physiology and Biochemistry Confocal Raman Microspectral Imaging Low-level Laser Therapy Spinal Cord Injury Photobiological Effects Pathobiological Analysis |
author_facet |
Yuze Gong Shuang Wang Zhuowen Liang Zhe Wang Xu Zhang Jie Li Jiwei Song Xueyu Hu Kaige Wang Qingli He Jintao Bai |
author_sort |
Yuze Gong |
title |
Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury |
title_short |
Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury |
title_full |
Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury |
title_fullStr |
Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury |
title_full_unstemmed |
Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury |
title_sort |
label-free spectral imaging unveils biochemical mechanisms of low-level laser therapy on spinal cord injury |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2018-09-01 |
description |
Background/Aims: Low-level laser therapy (LLLT) leads to complex photochemical responses during the healing process of spinal cord injury (SCI). Confocal Raman Microspectral Imaging (in combination with multivariate analysis) was adopted to illustrate the underlying biochemical mechanisms of LLLT treatment on a SCI rat model. Methods: Using transversal tissue sections, the Raman spectra can identify areas neighboring the injury site, glial scar, cavity, and unharmed white matter, as well as their correlated cellular alterations, such as demyelination and up-regulation of chondroitin sulfate proteoglycans (CSPGs). Multivariate data analysis methods are used to depict the underlying therapeutic effects by highlighting the detailed content and distribution variations of the biochemical constituents. Results: It is confirmed that photon-tissue interactions might lead to a decay of the inhibitory response to remyelination by suppressing CSPG expression, as also morphologically demonstrated by reduced glial scar and cavity areas. An inter-group comparison semi-quantitatively confirms changes in lipids, phosphatidic acid, CSPGs, and cholesterol during SCI and its LLLT treatment, paving the way for in vitro and in vivo understanding of the biochemical changes accompanying pathobiological SCI events. Conclusion: The achieved results in this work not only have once again proved the well-known cellular mechanisms of SCI, but further illustrate the underlying biochemical variability during LLLT treatment, which provide a sound basis for developing real-time Raman methodologies to monitor the efficacy of the SCI LLLT treatment. |
topic |
Confocal Raman Microspectral Imaging Low-level Laser Therapy Spinal Cord Injury Photobiological Effects Pathobiological Analysis |
url |
https://www.karger.com/Article/FullText/493295 |
work_keys_str_mv |
AT yuzegong labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT shuangwang labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT zhuowenliang labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT zhewang labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT xuzhang labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT jieli labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT jiweisong labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT xueyuhu labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT kaigewang labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT qinglihe labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury AT jintaobai labelfreespectralimagingunveilsbiochemicalmechanismsoflowlevellasertherapyonspinalcordinjury |
_version_ |
1724976593332338688 |