Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals.
Antimicrobial resistance genes can be found in all ecosystems, including those where antibiotic selective pressure has never been exerted. We investigated resistance genes in a collection of faecal samples of wildlife (non-human primates, mice), people and domestic animals (dogs, cats) in Côte d...
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2014-01-01
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doaj-5c0e2a0d896c4db8a6d43b9d9b9d68322021-03-03T20:11:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11354810.1371/journal.pone.0113548Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals.Katerina AlbrechtovaIvo PapousekHelene De NysMaude PaulyEtile AnohArsene MossounMonika DolejskaMartina MasarikovaSonya MetzgerEmmanuel Couacy-HymannChantal Akoua-KoffiRoman M WittigJiri KlimesAlois CizekFabian H LeendertzIvan LiterakAntimicrobial resistance genes can be found in all ecosystems, including those where antibiotic selective pressure has never been exerted. We investigated resistance genes in a collection of faecal samples of wildlife (non-human primates, mice), people and domestic animals (dogs, cats) in Côte d'Ivoire; in the chimpanzee research area of Taï National Park (TNP) and adjacent villages. Single bacteria isolates were collected from antibiotic-containing agar plates and subjected to molecular analysis to detect Enterobacteriaceae isolates with plasmid-mediated genes of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR). While the prevalence of ESBL-producing E. coli in the villages was 27% in people (n = 77) and 32% in dogs (n = 38), no ESBL-producer was found in wildlife of TNP (n = 75). PMQR genes, mainly represented by qnrS1, were also present in human- and dog-originating isolates from the villages (36% and 42% in people and dogs, respectively), but no qnrS has been found in the park. In TNP, different variants of qnrB were detected in Citrobacter freundii isolates originating non-human primates and mice. In conclusion, ESBL and PMQR genes frequently found in humans and domestic animals in the villages were rather exceptional in wildlife living in the protected area. Although people enter the park, the strict biosecurity levels they are obliged to follow probably impede transmission of bacteria between them and wildlife.https://doi.org/10.1371/journal.pone.0113548 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katerina Albrechtova Ivo Papousek Helene De Nys Maude Pauly Etile Anoh Arsene Mossoun Monika Dolejska Martina Masarikova Sonya Metzger Emmanuel Couacy-Hymann Chantal Akoua-Koffi Roman M Wittig Jiri Klimes Alois Cizek Fabian H Leendertz Ivan Literak |
spellingShingle |
Katerina Albrechtova Ivo Papousek Helene De Nys Maude Pauly Etile Anoh Arsene Mossoun Monika Dolejska Martina Masarikova Sonya Metzger Emmanuel Couacy-Hymann Chantal Akoua-Koffi Roman M Wittig Jiri Klimes Alois Cizek Fabian H Leendertz Ivan Literak Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals. PLoS ONE |
author_facet |
Katerina Albrechtova Ivo Papousek Helene De Nys Maude Pauly Etile Anoh Arsene Mossoun Monika Dolejska Martina Masarikova Sonya Metzger Emmanuel Couacy-Hymann Chantal Akoua-Koffi Roman M Wittig Jiri Klimes Alois Cizek Fabian H Leendertz Ivan Literak |
author_sort |
Katerina Albrechtova |
title |
Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals. |
title_short |
Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals. |
title_full |
Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals. |
title_fullStr |
Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals. |
title_full_unstemmed |
Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals. |
title_sort |
low rates of antimicrobial-resistant enterobacteriaceae in wildlife in taï national park, côte d'ivoire, surrounded by villages with high prevalence of multiresistant esbl-producing escherichia coli in people and domestic animals. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Antimicrobial resistance genes can be found in all ecosystems, including those where antibiotic selective pressure has never been exerted. We investigated resistance genes in a collection of faecal samples of wildlife (non-human primates, mice), people and domestic animals (dogs, cats) in Côte d'Ivoire; in the chimpanzee research area of Taï National Park (TNP) and adjacent villages. Single bacteria isolates were collected from antibiotic-containing agar plates and subjected to molecular analysis to detect Enterobacteriaceae isolates with plasmid-mediated genes of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR). While the prevalence of ESBL-producing E. coli in the villages was 27% in people (n = 77) and 32% in dogs (n = 38), no ESBL-producer was found in wildlife of TNP (n = 75). PMQR genes, mainly represented by qnrS1, were also present in human- and dog-originating isolates from the villages (36% and 42% in people and dogs, respectively), but no qnrS has been found in the park. In TNP, different variants of qnrB were detected in Citrobacter freundii isolates originating non-human primates and mice. In conclusion, ESBL and PMQR genes frequently found in humans and domestic animals in the villages were rather exceptional in wildlife living in the protected area. Although people enter the park, the strict biosecurity levels they are obliged to follow probably impede transmission of bacteria between them and wildlife. |
url |
https://doi.org/10.1371/journal.pone.0113548 |
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