Astrogliosis in an Experimental Model of Hypovitaminosis B12: A Cellular Basis of Neurological Disorders due to Cobalamin Deficiency

• Main Authors: Zuzanna Rzepka, Jakub Rok, Justyna Kowalska, Klaudia Banach, Justyna Magdalena Hermanowicz, Artur Beberok, Beata Sieklucka, Dorota Gryko, Dorota Wrześniok
• Format: Article
• Language: English
• Published: MDPI AG 2020-10-01
• Series: Cells
• Subjects: astrocytes; astrogliosis; neurological disorders; cobalamin; hypovitaminosis B12
• Online Access: https://www.mdpi.com/2073-4409/9/10/2261

Cobalamin deficiency affects human physiology with sequelae ranging from mild fatigue to severe neuropsychiatric abnormalities. The cellular and molecular aspects of the nervous system disorders associated with hypovitaminosis B12 remain largely unknown. Growing evidence indicates that astrogliosis is an underlying component of a wide range of neuropathologies. Previously, we developed an in vitro model of cobalamin deficiency in normal human astrocytes (NHA) by culturing the cells with <i>c</i>-lactam of hydroxycobalamin (<i>c</i>-lactam OH-Cbl). We revealed a non-apoptotic activation of caspases (3/7, 8, 9) in cobalamin-deficient NHA, which may suggest astrogliosis. The aim of the current study was to experimentally verify this hypothesis. We indicated an increase in the cellular expression of two astrogliosis markers: glial fibrillary acidic protein and vimentin in cobalamin-deficient NHA using Western blot analysis and immunocytochemistry with confocal laser scanning microscopy. In the next step of the study, we revealed <i>c</i>-lactam OH-Cbl as a potential non-toxic vitamin B12 antagonist in an in vivo model using zebrafish embryos. We believe that the presented results will contribute to a better understanding of the cellular mechanism underlying neurologic pathology due to cobalamin deficiency and will serve as a foundation for further studies.