Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques
Diagnosis of bowel diseases is often difficult and time consuming since it is not always possible to obtain adequate information by the conventional diagnostic methods to set up a diagnosis and exclude nongastrointestinal causes of symptoms. The aim of this study was to investigate the structure of...
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doaj-5c29c72ea46f4c8581b539069a60b31c2021-09-06T19:19:34ZengDe GruyterOpen Chemistry2391-54202018-03-0116123824510.1515/chem-2018-0024chem-2018-0024Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy TechniquesTomečková Vladimíra0Tóth Štefan1Tóth Tímea2Komanický Vladimír3Krajčíková Kristína4Široká Monika5Glinská Gabriela6Pella Dominik7Mašlanková Jana8Tomečko Marián9Hakim Tamer10Štefanič Peter11Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak Republic1st Department of Internal Medicine, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak Republic1st Department of Internal Medicine, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicInstitute of Physics, Faculty of Science, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicDepartment of Cardiology, East Slovak Institute of Cardiovascular Disease in Košice, Košice, Slovak RepublicDepartment of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicDepartment of Vascular Surgery, East Slovak Institute of Cardiovascular Disease in Košice, Košice, Slovak Republic1st Department of Internal Medicine, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Košice, Slovak RepublicDepartment of Vascular Surgery, East Slovak Institute of Cardiovascular Disease in Košice, Košice, Slovak RepublicDiagnosis of bowel diseases is often difficult and time consuming since it is not always possible to obtain adequate information by the conventional diagnostic methods to set up a diagnosis and exclude nongastrointestinal causes of symptoms. The aim of this study was to investigate the structure of blood serum samples of patients with selected intestinal diseases. The blood serum samples of patients (N=35) with selected diagnoses (mesenteric thrombosis, inflammatory bowel disease, duodenal ulcers, sepsis, enterorrhagia, sigmoid colon resection, small intestine cancer) and of healthy subjects were evaluated by synchronous fluorescence fingerprint and atomic force microscopy. Autofluorescence of blood serum studied at λex = 280 nm showed significant decrease of fluorescence intensity in patients with all types of diseases affecting bowels in comparison with the healthy control patients. The blood serum surface of ill patients showed significant differences in comparison with control group samples after atomic force microscopy evaluation as well. Irregularly placed small globular units of irregular shape in small amounts are possible to observe in patients with intestine ischemia. Fluorescence analysis and atomic force microscopy showed the ability to rapidly reflect qualitative and quantitative changes of proteins in blood serum samples of patients. These sensitive methods could be beneficial for monitoring the progression of both acute or chronic bowel diseases.https://doi.org/10.1515/chem-2018-0024bowel diseasesanalysisproteinssynchronous fluorescence fingerprintatomic force microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomečková Vladimíra Tóth Štefan Tóth Tímea Komanický Vladimír Krajčíková Kristína Široká Monika Glinská Gabriela Pella Dominik Mašlanková Jana Tomečko Marián Hakim Tamer Štefanič Peter |
spellingShingle |
Tomečková Vladimíra Tóth Štefan Tóth Tímea Komanický Vladimír Krajčíková Kristína Široká Monika Glinská Gabriela Pella Dominik Mašlanková Jana Tomečko Marián Hakim Tamer Štefanič Peter Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques Open Chemistry bowel diseases analysis proteins synchronous fluorescence fingerprint atomic force microscopy |
author_facet |
Tomečková Vladimíra Tóth Štefan Tóth Tímea Komanický Vladimír Krajčíková Kristína Široká Monika Glinská Gabriela Pella Dominik Mašlanková Jana Tomečko Marián Hakim Tamer Štefanič Peter |
author_sort |
Tomečková Vladimíra |
title |
Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques |
title_short |
Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques |
title_full |
Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques |
title_fullStr |
Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques |
title_full_unstemmed |
Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques |
title_sort |
analysis of bowel diseases from blood serum by autofluorescence and atomic force microscopy techniques |
publisher |
De Gruyter |
series |
Open Chemistry |
issn |
2391-5420 |
publishDate |
2018-03-01 |
description |
Diagnosis of bowel diseases is often difficult and time consuming since it is not always possible to obtain adequate information by the conventional diagnostic methods to set up a diagnosis and exclude nongastrointestinal causes of symptoms. The aim of this study was to investigate the structure of blood serum samples of patients with selected intestinal diseases. The blood serum samples of patients (N=35) with selected diagnoses (mesenteric thrombosis, inflammatory bowel disease, duodenal ulcers, sepsis, enterorrhagia, sigmoid colon resection, small intestine cancer) and of healthy subjects were evaluated by synchronous fluorescence fingerprint and atomic force microscopy. Autofluorescence of blood serum studied at λex = 280 nm showed significant decrease of fluorescence intensity in patients with all types of diseases affecting bowels in comparison with the healthy control patients. The blood serum surface of ill patients showed significant differences in comparison with control group samples after atomic force microscopy evaluation as well. Irregularly placed small globular units of irregular shape in small amounts are possible to observe in patients with intestine ischemia. Fluorescence analysis and atomic force microscopy showed the ability to rapidly reflect qualitative and quantitative changes of proteins in blood serum samples of patients. These sensitive methods could be beneficial for monitoring the progression of both acute or chronic bowel diseases. |
topic |
bowel diseases analysis proteins synchronous fluorescence fingerprint atomic force microscopy |
url |
https://doi.org/10.1515/chem-2018-0024 |
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