Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst
While the interactions of the gut microbiome and blood metabolome have been widely studied in polycystic ovary disease in women, follicular cysts of ewes have been scarcely investigated using these methods. In this study, the fecal microbiome and serum metabolome were used to compare between ewes di...
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doaj-5c29f138744a4b4ba56947e6eb977db52021-05-12T05:07:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-05-011210.3389/fmicb.2021.675480675480Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular CystTao Feng0Tao Feng1Tao Feng2Hongxiang Ding3Hongxiang Ding4Hongxiang Ding5Jing Wang6Wei Xu7Yan Liu8Yan Liu9Ákos Kenéz10Institute of Animal Husbandry and Veterinary Medicine (IAHVM), Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, ChinaJoint Laboratory of Animal Science Between IAHVM of BAAFS and Division of Agricultural Science and Natural Resource, Oklahoma State University, Beijing, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaInstitute of Animal Husbandry and Veterinary Medicine (IAHVM), Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, ChinaJoint Laboratory of Animal Science Between IAHVM of BAAFS and Division of Agricultural Science and Natural Resource, Oklahoma State University, Beijing, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaDepartment of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong, ChinaInstitute of Animal Husbandry and Veterinary Medicine (IAHVM), Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, ChinaJoint Laboratory of Animal Science Between IAHVM of BAAFS and Division of Agricultural Science and Natural Resource, Oklahoma State University, Beijing, ChinaDepartment of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong, ChinaWhile the interactions of the gut microbiome and blood metabolome have been widely studied in polycystic ovary disease in women, follicular cysts of ewes have been scarcely investigated using these methods. In this study, the fecal microbiome and serum metabolome were used to compare between ewes diagnosed with ovarian cystic follicles and ewes with normal follicles, to investigate alterations of the fecal bacterial community composition and metabolic parameters in relation to follicular cystogenesis. Ewes from the same feeding and management system were diagnosed with a follicular cyst (n = 6) or confirmed to have normal follicles (n = 6) by using a B-mode ultrasound scanner. Blood serum and fresh fecal samples of all ewes were collected and analyzed. The α-diversity of fecal microbiome did not differ significantly between follicular cyst ewes and normal follicle ewes. Three genera (Bacteroides, Anaerosporobacter, and Angelakisella) were identified and their balance differentiated between follicular cyst and normal follicle ewes. Alterations of several serum metabolite concentrations, belonging to lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, benzenoids, phenylpropanoids and polyketides, and organoheterocyclic compounds, were associated with the presence of a follicular cyst. Correlation analysis between fecal bacterial communities and serum metabolites indicated a positive correlation between Anaerosporobacter and several fatty acids, and a negative correlation between Bacteroides and L-proline. These observations provide new insights for the complex interactions of the gut microbiota and the host serum lipid profiles, and support gut microbiota as a potential strategy to treat and prevent follicular cysts in sheep.https://www.frontiersin.org/articles/10.3389/fmicb.2021.675480/fullsheepfollicular cystsmicrobial diversitymetabolomehost-microbiota interactions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tao Feng Tao Feng Tao Feng Hongxiang Ding Hongxiang Ding Hongxiang Ding Jing Wang Wei Xu Yan Liu Yan Liu Ákos Kenéz |
spellingShingle |
Tao Feng Tao Feng Tao Feng Hongxiang Ding Hongxiang Ding Hongxiang Ding Jing Wang Wei Xu Yan Liu Yan Liu Ákos Kenéz Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst Frontiers in Microbiology sheep follicular cysts microbial diversity metabolome host-microbiota interactions |
author_facet |
Tao Feng Tao Feng Tao Feng Hongxiang Ding Hongxiang Ding Hongxiang Ding Jing Wang Wei Xu Yan Liu Yan Liu Ákos Kenéz |
author_sort |
Tao Feng |
title |
Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst |
title_short |
Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst |
title_full |
Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst |
title_fullStr |
Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst |
title_full_unstemmed |
Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst |
title_sort |
alterations of serum metabolites and fecal microbiota involved in ewe follicular cyst |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-05-01 |
description |
While the interactions of the gut microbiome and blood metabolome have been widely studied in polycystic ovary disease in women, follicular cysts of ewes have been scarcely investigated using these methods. In this study, the fecal microbiome and serum metabolome were used to compare between ewes diagnosed with ovarian cystic follicles and ewes with normal follicles, to investigate alterations of the fecal bacterial community composition and metabolic parameters in relation to follicular cystogenesis. Ewes from the same feeding and management system were diagnosed with a follicular cyst (n = 6) or confirmed to have normal follicles (n = 6) by using a B-mode ultrasound scanner. Blood serum and fresh fecal samples of all ewes were collected and analyzed. The α-diversity of fecal microbiome did not differ significantly between follicular cyst ewes and normal follicle ewes. Three genera (Bacteroides, Anaerosporobacter, and Angelakisella) were identified and their balance differentiated between follicular cyst and normal follicle ewes. Alterations of several serum metabolite concentrations, belonging to lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, benzenoids, phenylpropanoids and polyketides, and organoheterocyclic compounds, were associated with the presence of a follicular cyst. Correlation analysis between fecal bacterial communities and serum metabolites indicated a positive correlation between Anaerosporobacter and several fatty acids, and a negative correlation between Bacteroides and L-proline. These observations provide new insights for the complex interactions of the gut microbiota and the host serum lipid profiles, and support gut microbiota as a potential strategy to treat and prevent follicular cysts in sheep. |
topic |
sheep follicular cysts microbial diversity metabolome host-microbiota interactions |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.675480/full |
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