Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1
In presence of oleate and taurocholate, differentiated CaCo-2 cell monolayers on membranes were able to assemble and secrete chylomicrons. Under these conditions, both cellular uptake and secretion into chylomicrons of β-carotene (β-C) were curvilinear, time-dependent (2–16 h), saturable, and concen...
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doaj-5c2f83462d2b4630bcc76b7ee78f77552021-04-27T04:39:46ZengElsevierJournal of Lipid Research0022-22752002-07-0143710861095Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1Alexandrine During0M. Mahmood Hussain1Diane W. Morel2Earl H. Harrison3To whom correspondence should be addressed; Human Nutrition Research Center, United States Department of Agriculture, Beltsville, Maryland 20705Departments of Anatomy & Cell Biology and Pediatrics, SUNY Downstate Medical Center, Brooklyn, New York 11203Department of Pharmacology, University of the Sciences in Philadelphia, Philadelphia, Pennsylvania 19404Human Nutrition Research Center, United States Department of Agriculture, Beltsville, Maryland 20705In presence of oleate and taurocholate, differentiated CaCo-2 cell monolayers on membranes were able to assemble and secrete chylomicrons. Under these conditions, both cellular uptake and secretion into chylomicrons of β-carotene (β-C) were curvilinear, time-dependent (2–16 h), saturable, and concentration-dependent (apparent Km of 7–10 μM) processes. Under linear concentration conditions at 16 h incubation, the extent of absorption of all-trans β-C was 11% (80% in chylomicrons), while those of 9-cis- and 13-cis-β-C were significantly lower (2–3%). The preferential uptake of the all-trans isomer was also shown in hepatic stellate HSC-T6 cells and in a cell-free system from rat liver (microsomes), but not in endothelial EAHY cells or U937 monocyte-macrophages. Moreover, extents of absorption of α-carotene (α-C), lutein (LUT), and lycopene (LYC) in CaCo-2 cells were 10%, 7%, and 2.5%, respectively. Marked carotenoid interactions were observed between LYC/β-C and β-C/α-C.The present results indicate that β-C conformation plays a major role in its intestinal absorption and that cis isomer discrimination is at the levels of cellular uptake and incorporation into chylomicrons. Moreover, the kinetics of cellular uptake and secretion of β-C, the inhibition of the intestinal absorption of one carotenoid by another, and the cellular specificity of isomer discrimination all suggest that carotenoid uptake by intestinal cells is a facilitated process.http://www.sciencedirect.com/science/article/pii/S0022227520314711cis isomersα-carotenelycopeneluteinchylomicronshuman intestinal model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandrine During M. Mahmood Hussain Diane W. Morel Earl H. Harrison |
spellingShingle |
Alexandrine During M. Mahmood Hussain Diane W. Morel Earl H. Harrison Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1 Journal of Lipid Research cis isomers α-carotene lycopene lutein chylomicrons human intestinal model |
author_facet |
Alexandrine During M. Mahmood Hussain Diane W. Morel Earl H. Harrison |
author_sort |
Alexandrine During |
title |
Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1 |
title_short |
Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1 |
title_full |
Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1 |
title_fullStr |
Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1 |
title_full_unstemmed |
Carotenoid uptake and secretion by CaCo-2 cells: β-carotene isomer selectivity and carotenoid interactions1 |
title_sort |
carotenoid uptake and secretion by caco-2 cells: β-carotene isomer selectivity and carotenoid interactions1 |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2002-07-01 |
description |
In presence of oleate and taurocholate, differentiated CaCo-2 cell monolayers on membranes were able to assemble and secrete chylomicrons. Under these conditions, both cellular uptake and secretion into chylomicrons of β-carotene (β-C) were curvilinear, time-dependent (2–16 h), saturable, and concentration-dependent (apparent Km of 7–10 μM) processes. Under linear concentration conditions at 16 h incubation, the extent of absorption of all-trans β-C was 11% (80% in chylomicrons), while those of 9-cis- and 13-cis-β-C were significantly lower (2–3%). The preferential uptake of the all-trans isomer was also shown in hepatic stellate HSC-T6 cells and in a cell-free system from rat liver (microsomes), but not in endothelial EAHY cells or U937 monocyte-macrophages. Moreover, extents of absorption of α-carotene (α-C), lutein (LUT), and lycopene (LYC) in CaCo-2 cells were 10%, 7%, and 2.5%, respectively. Marked carotenoid interactions were observed between LYC/β-C and β-C/α-C.The present results indicate that β-C conformation plays a major role in its intestinal absorption and that cis isomer discrimination is at the levels of cellular uptake and incorporation into chylomicrons. Moreover, the kinetics of cellular uptake and secretion of β-C, the inhibition of the intestinal absorption of one carotenoid by another, and the cellular specificity of isomer discrimination all suggest that carotenoid uptake by intestinal cells is a facilitated process. |
topic |
cis isomers α-carotene lycopene lutein chylomicrons human intestinal model |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520314711 |
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