Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation
Patients with metabolic syndrome are at an increased risk of developing type 2 diabetes and cardiovascular diseases. The principal risk factor for development of metabolic syndrome is obesity, defined as a state of pathological hyperplasia or/and hypertrophy of adipose tissue. The number of mature a...
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doaj-5c334e668ab541b7a98b84959dd2d7632020-11-25T01:44:28ZengMDPI AGNutrients2072-66432019-09-011110230710.3390/nu11102307nu11102307Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte DifferentiationLiang-Yi Wu0Chien-Wei Chen1Luen-Kui Chen2Hsiang-Yun Chou3Chih-Ling Chang4Chi-Chang Juan5Department of Bioscience Technology, College of Science, Chung-Yuan Christian University, Chung Li 32023, TaiwanDepartment of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, TaiwanDepartment of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, TaiwanDepartment of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, TaiwanDepartment of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, TaiwanDepartment of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, TaiwanPatients with metabolic syndrome are at an increased risk of developing type 2 diabetes and cardiovascular diseases. The principal risk factor for development of metabolic syndrome is obesity, defined as a state of pathological hyperplasia or/and hypertrophy of adipose tissue. The number of mature adipocytes is determined by adipocyte differentiation from preadipocytes. The purpose of the present study is to investigate the effects of curcumin on adipogenesis and the underlying mechanism. To examine cell toxicity of curcumin, 3T3-L1 preadipocytes were treated with 0−50 µM curcumin for 24, 48, or 72 h, then cell viability was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The effect of curcumin on the cell cycle was determined by flow cytometry. Curcumin-induced cell apoptosis was determined by the TUNEL assay and curcumin-induced caspase activation was measured by immunoblotting. The effect of curcumin on adipocyte differentiation was determined by measuring mitotic clonal expansion (MCE), expression of adipogenic transcription factors, and lipid accumulation. Results showed the viability of preadipocytes was significantly decreased by treatment with 30 µM curcumin, a concentration that caused apoptosis in preadipocytes, as assessed by the TUNEL assay, and caused activation of caspases 8, 9, and 3. A non-cytotoxic dose of curcumin (15 µM) inhibited MCE, downregulated the expression of PPARγ and C/EBPα, prevented differentiation medium-induced β-catenin downregulation, and decreased the lipid accumulation in 3T3-L1 adipocytes. In conclusion, our data show that curcumin can induce preadipocyte apoptosis and inhibit adipocyte differentiation, leading to suppression of adipogenesis.https://www.mdpi.com/2072-6643/11/10/2307curcuminadipocytesadipogenesisapoptosismitotic clonal expansiondifferentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liang-Yi Wu Chien-Wei Chen Luen-Kui Chen Hsiang-Yun Chou Chih-Ling Chang Chi-Chang Juan |
spellingShingle |
Liang-Yi Wu Chien-Wei Chen Luen-Kui Chen Hsiang-Yun Chou Chih-Ling Chang Chi-Chang Juan Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation Nutrients curcumin adipocytes adipogenesis apoptosis mitotic clonal expansion differentiation |
author_facet |
Liang-Yi Wu Chien-Wei Chen Luen-Kui Chen Hsiang-Yun Chou Chih-Ling Chang Chi-Chang Juan |
author_sort |
Liang-Yi Wu |
title |
Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation |
title_short |
Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation |
title_full |
Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation |
title_fullStr |
Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation |
title_full_unstemmed |
Curcumin Attenuates Adipogenesis by Inducing Preadipocyte Apoptosis and Inhibiting Adipocyte Differentiation |
title_sort |
curcumin attenuates adipogenesis by inducing preadipocyte apoptosis and inhibiting adipocyte differentiation |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-09-01 |
description |
Patients with metabolic syndrome are at an increased risk of developing type 2 diabetes and cardiovascular diseases. The principal risk factor for development of metabolic syndrome is obesity, defined as a state of pathological hyperplasia or/and hypertrophy of adipose tissue. The number of mature adipocytes is determined by adipocyte differentiation from preadipocytes. The purpose of the present study is to investigate the effects of curcumin on adipogenesis and the underlying mechanism. To examine cell toxicity of curcumin, 3T3-L1 preadipocytes were treated with 0−50 µM curcumin for 24, 48, or 72 h, then cell viability was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The effect of curcumin on the cell cycle was determined by flow cytometry. Curcumin-induced cell apoptosis was determined by the TUNEL assay and curcumin-induced caspase activation was measured by immunoblotting. The effect of curcumin on adipocyte differentiation was determined by measuring mitotic clonal expansion (MCE), expression of adipogenic transcription factors, and lipid accumulation. Results showed the viability of preadipocytes was significantly decreased by treatment with 30 µM curcumin, a concentration that caused apoptosis in preadipocytes, as assessed by the TUNEL assay, and caused activation of caspases 8, 9, and 3. A non-cytotoxic dose of curcumin (15 µM) inhibited MCE, downregulated the expression of PPARγ and C/EBPα, prevented differentiation medium-induced β-catenin downregulation, and decreased the lipid accumulation in 3T3-L1 adipocytes. In conclusion, our data show that curcumin can induce preadipocyte apoptosis and inhibit adipocyte differentiation, leading to suppression of adipogenesis. |
topic |
curcumin adipocytes adipogenesis apoptosis mitotic clonal expansion differentiation |
url |
https://www.mdpi.com/2072-6643/11/10/2307 |
work_keys_str_mv |
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