Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines
This study evaluated the protective effect of proanthocyanidins (PCs) on reducing apoptosis in the mouse intestinal epithelial cell model MODE-K exposed to zearalenone (ZEA) through inhibition of the endoplasmic reticulum stress (ERS)-induced apoptosis pathway. Our results showed that PCs could redu...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-06-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/23/7/1508 |
id |
doaj-5c35c05294c54a5d94fe31297ee91447 |
---|---|
record_format |
Article |
spelling |
doaj-5c35c05294c54a5d94fe31297ee914472020-11-24T21:21:30ZengMDPI AGMolecules1420-30492018-06-01237150810.3390/molecules23071508molecules23071508Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small IntestinesMiao Long0Xinliang Chen1Nan Wang2Mingyang Wang3Jiawen Pan4Jingjing Tong5Peng Li6Shuhua Yang7Jianbin He8Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaThis study evaluated the protective effect of proanthocyanidins (PCs) on reducing apoptosis in the mouse intestinal epithelial cell model MODE-K exposed to zearalenone (ZEA) through inhibition of the endoplasmic reticulum stress (ERS)-induced apoptosis pathway. Our results showed that PCs could reduce the rate of apoptosis in MODE-K cells exposed to ZEA (p < 0.01). PCs significantly increased the ZEA-induced antioxidant protective effects on the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and on the content of GSH. PCs also significantly decreased the ZEA-induced increase in the content of malondialdehyde (MDA). The analysis indicated that ZEA increased both mRNA and protein expression levels of C/EBP homologous protein (CHOP), GRP78, c-Jun N-terminal kinase (JNK), and cysteinyl aspartate specific proteinase 12 (caspase-12) (p < 0.05), which are related to the ERS-induced apoptosis pathway. ZEA decreased levels of the pro-apoptotic related protein Bcl-2 (p < 0.05) and increased the anti-apoptotic related protein Bax (p < 0.05). Co-treatment with PCs was also shown to significantly reverse the expression levels of these proteins in MODE-K cells. The results demonstrated that PCs could protect MODE-K cells from oxidative stress and apoptosis induced by ZEA. The underlying mechanism may be that PCs can alleviate apoptosis in mouse intestinal epithelial cells by inhibition of the ERS-induced apoptosis pathway.http://www.mdpi.com/1420-3049/23/7/1508proanthocyanidinszearalenoneoxidative damageendoplasmic reticulum stressintestinal epithelial cellsmiceapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miao Long Xinliang Chen Nan Wang Mingyang Wang Jiawen Pan Jingjing Tong Peng Li Shuhua Yang Jianbin He |
spellingShingle |
Miao Long Xinliang Chen Nan Wang Mingyang Wang Jiawen Pan Jingjing Tong Peng Li Shuhua Yang Jianbin He Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines Molecules proanthocyanidins zearalenone oxidative damage endoplasmic reticulum stress intestinal epithelial cells mice apoptosis |
author_facet |
Miao Long Xinliang Chen Nan Wang Mingyang Wang Jiawen Pan Jingjing Tong Peng Li Shuhua Yang Jianbin He |
author_sort |
Miao Long |
title |
Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines |
title_short |
Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines |
title_full |
Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines |
title_fullStr |
Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines |
title_full_unstemmed |
Proanthocyanidins Protect Epithelial Cells from Zearalenone-Induced Apoptosis via Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis Pathways in Mouse Small Intestines |
title_sort |
proanthocyanidins protect epithelial cells from zearalenone-induced apoptosis via inhibition of endoplasmic reticulum stress-induced apoptosis pathways in mouse small intestines |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-06-01 |
description |
This study evaluated the protective effect of proanthocyanidins (PCs) on reducing apoptosis in the mouse intestinal epithelial cell model MODE-K exposed to zearalenone (ZEA) through inhibition of the endoplasmic reticulum stress (ERS)-induced apoptosis pathway. Our results showed that PCs could reduce the rate of apoptosis in MODE-K cells exposed to ZEA (p < 0.01). PCs significantly increased the ZEA-induced antioxidant protective effects on the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and on the content of GSH. PCs also significantly decreased the ZEA-induced increase in the content of malondialdehyde (MDA). The analysis indicated that ZEA increased both mRNA and protein expression levels of C/EBP homologous protein (CHOP), GRP78, c-Jun N-terminal kinase (JNK), and cysteinyl aspartate specific proteinase 12 (caspase-12) (p < 0.05), which are related to the ERS-induced apoptosis pathway. ZEA decreased levels of the pro-apoptotic related protein Bcl-2 (p < 0.05) and increased the anti-apoptotic related protein Bax (p < 0.05). Co-treatment with PCs was also shown to significantly reverse the expression levels of these proteins in MODE-K cells. The results demonstrated that PCs could protect MODE-K cells from oxidative stress and apoptosis induced by ZEA. The underlying mechanism may be that PCs can alleviate apoptosis in mouse intestinal epithelial cells by inhibition of the ERS-induced apoptosis pathway. |
topic |
proanthocyanidins zearalenone oxidative damage endoplasmic reticulum stress intestinal epithelial cells mice apoptosis |
url |
http://www.mdpi.com/1420-3049/23/7/1508 |
work_keys_str_mv |
AT miaolong proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT xinliangchen proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT nanwang proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT mingyangwang proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT jiawenpan proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT jingjingtong proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT pengli proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT shuhuayang proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines AT jianbinhe proanthocyanidinsprotectepithelialcellsfromzearalenoneinducedapoptosisviainhibitionofendoplasmicreticulumstressinducedapoptosispathwaysinmousesmallintestines |
_version_ |
1725999616210501632 |