Sox9a, not sox9b is required for normal cartilage development in zebrafish
Sox9 is a multifunctional gene and plays crucial roles in vertebrate development including chondrogensis. In teleost, due to the genome duplication event, there are two co-orthologs sox9a and sox9b. In this study, CRISPR/Cas9 technology was performed to disrupt the function of either sox9a or sox9b....
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doaj-5c3b04fc4d0c4827ad0827220606211a2021-04-28T06:09:40ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2021-05-0163254259Sox9a, not sox9b is required for normal cartilage development in zebrafishQiaohong Lin0Yan He1Jian-Fang Gui2Jie Mei3College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, 430070, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Corresponding author. College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.Sox9 is a multifunctional gene and plays crucial roles in vertebrate development including chondrogensis. In teleost, due to the genome duplication event, there are two co-orthologs sox9a and sox9b. In this study, CRISPR/Cas9 technology was performed to disrupt the function of either sox9a or sox9b. All sox9a mutants (sox9aΔ10 and sox9aΔ67) and sox9b mutants (sox9bΔ11 and sox9bΔ20) lost HMG domain and Q/S domain, however, only sox9a mutant larvae had mis-shaped pectoral fins and lacked the scapulocoracoid cartilage. sox9b mutant larvae showed normal cartilages similar to wild type larvae. The results suggested that sox9a, not sox9b was required for cartilage development in zebrafish, which was different from the sox9b-mutant phenotype induced by N-ethyl-N-nitrosourea (ENU) treatment, gamma radiation treatment or morpholino injection. This study confirmed that ancestral sox9 gene functions partitioned between the two paralogs in zebrafish.http://www.sciencedirect.com/science/article/pii/S2468550X19302175sox9asox9bCartilage developmentZebrafish |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiaohong Lin Yan He Jian-Fang Gui Jie Mei |
spellingShingle |
Qiaohong Lin Yan He Jian-Fang Gui Jie Mei Sox9a, not sox9b is required for normal cartilage development in zebrafish Aquaculture and Fisheries sox9a sox9b Cartilage development Zebrafish |
author_facet |
Qiaohong Lin Yan He Jian-Fang Gui Jie Mei |
author_sort |
Qiaohong Lin |
title |
Sox9a, not sox9b is required for normal cartilage development in zebrafish |
title_short |
Sox9a, not sox9b is required for normal cartilage development in zebrafish |
title_full |
Sox9a, not sox9b is required for normal cartilage development in zebrafish |
title_fullStr |
Sox9a, not sox9b is required for normal cartilage development in zebrafish |
title_full_unstemmed |
Sox9a, not sox9b is required for normal cartilage development in zebrafish |
title_sort |
sox9a, not sox9b is required for normal cartilage development in zebrafish |
publisher |
KeAi Communications Co., Ltd. |
series |
Aquaculture and Fisheries |
issn |
2468-550X |
publishDate |
2021-05-01 |
description |
Sox9 is a multifunctional gene and plays crucial roles in vertebrate development including chondrogensis. In teleost, due to the genome duplication event, there are two co-orthologs sox9a and sox9b. In this study, CRISPR/Cas9 technology was performed to disrupt the function of either sox9a or sox9b. All sox9a mutants (sox9aΔ10 and sox9aΔ67) and sox9b mutants (sox9bΔ11 and sox9bΔ20) lost HMG domain and Q/S domain, however, only sox9a mutant larvae had mis-shaped pectoral fins and lacked the scapulocoracoid cartilage. sox9b mutant larvae showed normal cartilages similar to wild type larvae. The results suggested that sox9a, not sox9b was required for cartilage development in zebrafish, which was different from the sox9b-mutant phenotype induced by N-ethyl-N-nitrosourea (ENU) treatment, gamma radiation treatment or morpholino injection. This study confirmed that ancestral sox9 gene functions partitioned between the two paralogs in zebrafish. |
topic |
sox9a sox9b Cartilage development Zebrafish |
url |
http://www.sciencedirect.com/science/article/pii/S2468550X19302175 |
work_keys_str_mv |
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