The effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation

The effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation

Liberase HI has many advantages over traditional collagenases. Liberase performs the rapid dissociation of human pancreata and allows for the preservation of the morphological and functional integrity of isolated islets. The aim of this study was to investigate the effect of different concentrations...

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Bibliographic Details
Main Authors: Nikolić D., Đorđević P.B., Dimitrijević-Srecković Vesna, Paunović I., Kalezić Nevena, Popović S., Stefanović D., Džingalašević Marina
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2010-01-01
Series:Archives of Biological Sciences
Subjects:
Liberase HI
yield
insulin secretion
human adult pancreatic islets
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2010/0354-46641003833N.pdf
Description
Summary:Liberase HI has many advantages over traditional collagenases. Liberase performs the rapid dissociation of human pancreata and allows for the preservation of the morphological and functional integrity of isolated islets. The aim of this study was to investigate the effect of different concentrations of Liberase HI (1.5, 2.5 and 3.5 mg/ml) on the yield, viability and function of islets isolated by a non-automated method. Based on our results, the Liberase HI concentration 1.5 mg/ml is recommended for this method of isolation. .
ISSN:0354-4664

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