Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

• Main Authors: Naoko Imagawa, Kazuya Inoue, Keisuke Matsumoto, Ayako Ochi, Michi Omori, Kayoko Yamamoto, Yoichiro Nakajima, Nahoko Kato-Kogoe, Hiroyuki Nakano, Tomiharu Matsushita, Seiji Yamaguchi, Phuc Thi Minh Le, Shinpei Maruyama, Takaaki Ueno
• Format: Article
• Language: English
• Published: MDPI AG 2020-11-01
• Series: Materials
• Subjects: titanium; bone bonding; selective laser melting; mixed-acid and heat treatment; additive manufacturing; custom-made plate
• Online Access: https://www.mdpi.com/1996-1944/13/22/5104

The additive manufacturing (AM) technique has attracted attention as one of the fully customizable medical material technologies. In addition, the development of new surface treatments has been investigated to improve the osteogenic ability of the AM titanium (Ti) plate. The purpose of this study was to evaluate the osteogenic activity of the AM Ti with mixed-acid and heat (MAH) treatment. Fully customized AM Ti plates were created with a curvature suitable for rat calvarial bone, and they were examined in a group implanted with the MAH-treated Ti in comparison with the untreated (UN) group. The AM Ti plates were fixed to the surface of rat calvarial bone, followed by extraction of the calvarial bone 1, 4, 8, and 12 weeks after implantation. The bonding between the bone and Ti was evaluated mechanically. In addition, AM Ti plates removed from the bone were examined histologically by electron microscopy and Villanueva–Goldner stain. The mechanical evaluation showed significantly stronger bone-bonding in the MAH group than in the UN group. In addition, active bone formation was seen histologically in the MAH group. Therefore, these findings indicate that MAH resulted in rapid and strong bonding between cortical bone and Ti.