Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

• Main Authors: Long-Biao Guo, Guan-Lin Xie, Guo-Chang Sun, Muhammad Ibrahim, Li-Rong Han, Fei Xu, Yang-Li Wang, Yuan Fang, Chang-Lin Shan, Qing Zhou, Bin Li
• Format: Article
• Language: English
• Published: MDPI AG 2013-05-01
• Series: Marine Drugs
• Subjects: antibacterial activity; characterization; chitosan; cross-link; glutaraldehyde
• Online Access: http://www.mdpi.com/1660-3397/11/5/1534

This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR) band at 1559 cm−1, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.