Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats
<p>Abstract</p> <p>Background</p> <p>It has been reported that the P2Y<sub>12 </sub>receptor (P2Y<sub>12</sub>R) is involved in satellite glial cells (SGCs) activation, indicating that P2Y<sub>12</sub>R expressed in SGCs may play func...
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doaj-5c93b277091049a08ffc9eae715bb07b2020-11-25T03:11:21ZengSAGE PublishingMolecular Pain1744-80692012-03-01812310.1186/1744-8069-8-23Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in ratsKatagiri AyanoShinoda MasamichiHonda KuniyaToyofuku AkiraSessle Barry JIwata Koichi<p>Abstract</p> <p>Background</p> <p>It has been reported that the P2Y<sub>12 </sub>receptor (P2Y<sub>12</sub>R) is involved in satellite glial cells (SGCs) activation, indicating that P2Y<sub>12</sub>R expressed in SGCs may play functional roles in orofacial neuropathic pain mechanisms. However, the involvement of P2Y<sub>12</sub>R in orofacial neuropathic pain mechanisms is still unknown. We therefore studied the reflex to noxious mechanical or heat stimulation of the tongue, P2Y<sub>12</sub>R and glial fibrillary acidic protein (GFAP) immunohistochemistries in the trigeminal ganglion (TG) in a rat model of unilateral lingual nerve crush (LNC) to evaluate role of P2Y<sub>12</sub>R in SGC in lingual neuropathic pain.</p> <p>Results</p> <p>The head-withdrawal reflex thresholds to mechanical and heat stimulation of the lateral tongue were significantly decreased in LNC-rats compared to sham-rats. These nocifensive effects were apparent on day 1 after LNC and lasted for 17 days. On days 3, 9, 15 and 21 after LNC, the mean relative number of TG neurons encircled with GFAP-immunoreactive (IR) cells significantly increased in the ophthalmic, maxillary and mandibular branch regions of TG. On day 3 after LNC, P2Y<sub>12</sub>R expression occurred in GFAP-IR cells but not neuronal nuclei (NeuN)-IR cells (i.e. neurons) in TG. After 3 days of successive administration of the P2Y<sub>12</sub>R antagonist MRS2395 into TG in LNC-rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly decreased coincident with a significant reversal of the lowered head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue compared to vehicle-injected rats. Furthermore, after 3 days of successive administration of the P2YR agonist 2-MeSADP into the TG in naïve rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly increased and head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue were significantly decreased in a dose-dependent manner compared to vehicle-injected rats.</p> <p>Conclusions</p> <p>The present findings provide the first evidence that the activation of P2Y<sub>12</sub>R in SGCs of TG following lingual nerve injury is involved in the enhancement of TG neuron activity and nocifensive reflex behavior, resulting in neuropathic pain in the tongue.</p> http://www.molecularpain.com/content/8/1/23Neuron-Glia interactionsLingual nerve injuryMechanical allodyniaHeat hyperalgesiaPurinergic receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katagiri Ayano Shinoda Masamichi Honda Kuniya Toyofuku Akira Sessle Barry J Iwata Koichi |
spellingShingle |
Katagiri Ayano Shinoda Masamichi Honda Kuniya Toyofuku Akira Sessle Barry J Iwata Koichi Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats Molecular Pain Neuron-Glia interactions Lingual nerve injury Mechanical allodynia Heat hyperalgesia Purinergic receptor |
author_facet |
Katagiri Ayano Shinoda Masamichi Honda Kuniya Toyofuku Akira Sessle Barry J Iwata Koichi |
author_sort |
Katagiri Ayano |
title |
Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats |
title_short |
Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats |
title_full |
Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats |
title_fullStr |
Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats |
title_full_unstemmed |
Satellite glial cell P2Y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats |
title_sort |
satellite glial cell p2y<sub>12 </sub>receptor in the trigeminal ganglion is involved in lingual neuropathic pain mechanisms in rats |
publisher |
SAGE Publishing |
series |
Molecular Pain |
issn |
1744-8069 |
publishDate |
2012-03-01 |
description |
<p>Abstract</p> <p>Background</p> <p>It has been reported that the P2Y<sub>12 </sub>receptor (P2Y<sub>12</sub>R) is involved in satellite glial cells (SGCs) activation, indicating that P2Y<sub>12</sub>R expressed in SGCs may play functional roles in orofacial neuropathic pain mechanisms. However, the involvement of P2Y<sub>12</sub>R in orofacial neuropathic pain mechanisms is still unknown. We therefore studied the reflex to noxious mechanical or heat stimulation of the tongue, P2Y<sub>12</sub>R and glial fibrillary acidic protein (GFAP) immunohistochemistries in the trigeminal ganglion (TG) in a rat model of unilateral lingual nerve crush (LNC) to evaluate role of P2Y<sub>12</sub>R in SGC in lingual neuropathic pain.</p> <p>Results</p> <p>The head-withdrawal reflex thresholds to mechanical and heat stimulation of the lateral tongue were significantly decreased in LNC-rats compared to sham-rats. These nocifensive effects were apparent on day 1 after LNC and lasted for 17 days. On days 3, 9, 15 and 21 after LNC, the mean relative number of TG neurons encircled with GFAP-immunoreactive (IR) cells significantly increased in the ophthalmic, maxillary and mandibular branch regions of TG. On day 3 after LNC, P2Y<sub>12</sub>R expression occurred in GFAP-IR cells but not neuronal nuclei (NeuN)-IR cells (i.e. neurons) in TG. After 3 days of successive administration of the P2Y<sub>12</sub>R antagonist MRS2395 into TG in LNC-rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly decreased coincident with a significant reversal of the lowered head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue compared to vehicle-injected rats. Furthermore, after 3 days of successive administration of the P2YR agonist 2-MeSADP into the TG in naïve rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly increased and head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue were significantly decreased in a dose-dependent manner compared to vehicle-injected rats.</p> <p>Conclusions</p> <p>The present findings provide the first evidence that the activation of P2Y<sub>12</sub>R in SGCs of TG following lingual nerve injury is involved in the enhancement of TG neuron activity and nocifensive reflex behavior, resulting in neuropathic pain in the tongue.</p> |
topic |
Neuron-Glia interactions Lingual nerve injury Mechanical allodynia Heat hyperalgesia Purinergic receptor |
url |
http://www.molecularpain.com/content/8/1/23 |
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