Proteolytic activity of the proteasome is required for female insect reproduction
Non-ATPase regulatory subunits (Rpns) are components of the 26S proteasome involved in polyubiquitinated substrate recognition and deubiquitination in eukaryotes. Here, we identified 15 homologues sequences of Rpn and associated genes by searching the genome and transcriptome databases of the brown...
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The Royal Society
2021-02-01
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Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200251 |
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doaj-5ccc86af7d8f47cab124059b287e2afa2021-03-15T16:10:34ZengThe Royal SocietyOpen Biology2046-24412021-02-0111210.1098/rsob.200251200251Proteolytic activity of the proteasome is required for female insect reproductionWei WangRui-Rui YangLu-Yao PengLu ZhangYue-Lin YaoYan-Yuan BaoNon-ATPase regulatory subunits (Rpns) are components of the 26S proteasome involved in polyubiquitinated substrate recognition and deubiquitination in eukaryotes. Here, we identified 15 homologues sequences of Rpn and associated genes by searching the genome and transcriptome databases of the brown planthopper, Nilaparvata lugens, a hemipteran rice pest. Temporospatial analysis showed that NlRpn genes were significantly highly expressed in eggs and ovaries but were less-highly expressed in males. RNA interference-mediated depletion of NlRpn genes decreased the proteolytic activity of proteasome and impeded the transcription of lipase and vitellogenin genes in the fat bodies and ovaries in adult females, and reduced the triglyceride content in the ovaries. Decrease of the proteolytic activity of the proteasome via knockdown of NlRpns also inhibited the transcription of halloween genes, including NlCYP307A2, NlCYP306A2 and NlCYP314A1, in the 20-hydroxyecdysone (20E) biosynthetic pathway in the ovaries, reduced 20E production in adult females, and impaired ovarian development and oocyte maturation, resulting in reduced fecundity. These novel findings indicate that the proteolytic activity of the proteasome is required for female reproductive processes in N. lugens, thus furthering our understanding of the reproductive and developmental strategies in insects.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200251non-atpase regulatory subunitproteolytic activityreproductionoocyte maturationovulation and hatchingnilaparvata lugens |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Wang Rui-Rui Yang Lu-Yao Peng Lu Zhang Yue-Lin Yao Yan-Yuan Bao |
spellingShingle |
Wei Wang Rui-Rui Yang Lu-Yao Peng Lu Zhang Yue-Lin Yao Yan-Yuan Bao Proteolytic activity of the proteasome is required for female insect reproduction Open Biology non-atpase regulatory subunit proteolytic activity reproduction oocyte maturation ovulation and hatching nilaparvata lugens |
author_facet |
Wei Wang Rui-Rui Yang Lu-Yao Peng Lu Zhang Yue-Lin Yao Yan-Yuan Bao |
author_sort |
Wei Wang |
title |
Proteolytic activity of the proteasome is required for female insect reproduction |
title_short |
Proteolytic activity of the proteasome is required for female insect reproduction |
title_full |
Proteolytic activity of the proteasome is required for female insect reproduction |
title_fullStr |
Proteolytic activity of the proteasome is required for female insect reproduction |
title_full_unstemmed |
Proteolytic activity of the proteasome is required for female insect reproduction |
title_sort |
proteolytic activity of the proteasome is required for female insect reproduction |
publisher |
The Royal Society |
series |
Open Biology |
issn |
2046-2441 |
publishDate |
2021-02-01 |
description |
Non-ATPase regulatory subunits (Rpns) are components of the 26S proteasome involved in polyubiquitinated substrate recognition and deubiquitination in eukaryotes. Here, we identified 15 homologues sequences of Rpn and associated genes by searching the genome and transcriptome databases of the brown planthopper, Nilaparvata lugens, a hemipteran rice pest. Temporospatial analysis showed that NlRpn genes were significantly highly expressed in eggs and ovaries but were less-highly expressed in males. RNA interference-mediated depletion of NlRpn genes decreased the proteolytic activity of proteasome and impeded the transcription of lipase and vitellogenin genes in the fat bodies and ovaries in adult females, and reduced the triglyceride content in the ovaries. Decrease of the proteolytic activity of the proteasome via knockdown of NlRpns also inhibited the transcription of halloween genes, including NlCYP307A2, NlCYP306A2 and NlCYP314A1, in the 20-hydroxyecdysone (20E) biosynthetic pathway in the ovaries, reduced 20E production in adult females, and impaired ovarian development and oocyte maturation, resulting in reduced fecundity. These novel findings indicate that the proteolytic activity of the proteasome is required for female reproductive processes in N. lugens, thus furthering our understanding of the reproductive and developmental strategies in insects. |
topic |
non-atpase regulatory subunit proteolytic activity reproduction oocyte maturation ovulation and hatching nilaparvata lugens |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200251 |
work_keys_str_mv |
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