Testing Method of Rape Root Exudates by GC-MS Analysis

• Main Authors: ZHANG Hong, GAO Ya-jun, AN Rong
• Format: Article
• Language: zho
• Published: Agro-Environmental Protection Institute, Ministry of Agriculture 2014-06-01
• Series: Journal of Agricultural Resources and Environment
• Subjects: Rape; root exudation; GC-MS; allelopathic effect
• Online Access: http://www.aed.org.cn/nyzyyhjxb/html/2014/3/20140314.htm

In order to establish a method for the detection of rape root exudates, the hydroponic experiment was conducted using CH2 Cl2 extract to detect, isolate and identify root exudates of rape seedlings by GC-MS. The results showed that the detected peaks had more densely distributed and relatively good separation of peaks according to the composition of the testing substance by using a suitable solvent delay stages and refining temperature program. From the results, rape seedling root exudates included hydrocarbons, alcohols, esters and acids, while compounds of hydrocarbons and alcohols had relatively high levels. Most of these substances had allelopathic effects which had a certain impact on the environment in adversity plant growth and development.