The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin
Natural coagulants are of organic nature and can increase the organic load of treated water and thus, they require purification in order to remove compounds that do not have coagulation activity. In this work, natural coagulant was extracted from 50 g L-1 of ground common bean with 0.5 mol L-1 NaCl....
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Serbian Chemical Society
2020-01-01
|
Series: | Journal of the Serbian Chemical Society |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51392000031P.pdf |
id |
doaj-5cd47f20d3cb49e39c246704fab662ca |
---|---|
record_format |
Article |
spelling |
doaj-5cd47f20d3cb49e39c246704fab662ca2021-02-05T08:15:02ZengSerbian Chemical Society Journal of the Serbian Chemical Society0352-51391820-74212020-01-0185121643165510.2298/JSC200311031P0352-51392000031PThe purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resinProdanović Jelena M.0Šćiban Marina B.1Kukić Dragana V.2Vasić Vesna M.3Blagojev Nevena T.4Antov Mirjana G.5Faculty of Technology Novi Sad, University of Novi Sad, Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, Novi Sad, SerbiaNatural coagulants are of organic nature and can increase the organic load of treated water and thus, they require purification in order to remove compounds that do not have coagulation activity. In this work, natural coagulant was extracted from 50 g L-1 of ground common bean with 0.5 mol L-1 NaCl. Proteins from this crude extract were precipitated by addition of ammonium sulphate. After the precipitation, separation and resolution of proteins, further purification was performed using the anion-exchange resin Amberlite IRA 958 Cl in a batch process. Partially purified coagulant eluted with 2 mol L-1 NaCl solution achieved the highest coagulation activity of 53.3 % at a dose of 1 mL L-1 although it contained the lowest amount of proteins, but a slightly lower coagulation activity of 49.8 % was achieved at more than 5 times lower dosage of the same fraction. The organic load in treated water when the purified fraction was applied as coagulant was almost 4 times lower than in case of the crude extract as coagulant.http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51392000031P.pdfproteinscoagulation activitywater clarificationorganic load |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Prodanović Jelena M. Šćiban Marina B. Kukić Dragana V. Vasić Vesna M. Blagojev Nevena T. Antov Mirjana G. |
spellingShingle |
Prodanović Jelena M. Šćiban Marina B. Kukić Dragana V. Vasić Vesna M. Blagojev Nevena T. Antov Mirjana G. The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin Journal of the Serbian Chemical Society proteins coagulation activity water clarification organic load |
author_facet |
Prodanović Jelena M. Šćiban Marina B. Kukić Dragana V. Vasić Vesna M. Blagojev Nevena T. Antov Mirjana G. |
author_sort |
Prodanović Jelena M. |
title |
The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin |
title_short |
The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin |
title_full |
The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin |
title_fullStr |
The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin |
title_full_unstemmed |
The purification of natural coagulant extracted from common bean on IRA 958 Cl anion exchange resin |
title_sort |
purification of natural coagulant extracted from common bean on ira 958 cl anion exchange resin |
publisher |
Serbian Chemical Society |
series |
Journal of the Serbian Chemical Society |
issn |
0352-5139 1820-7421 |
publishDate |
2020-01-01 |
description |
Natural coagulants are of organic nature and can increase the organic load of treated water and thus, they require purification in order to remove compounds that do not have coagulation activity. In this work, natural coagulant was extracted from 50 g L-1 of ground common bean with 0.5 mol L-1 NaCl. Proteins from this crude extract were precipitated by addition of ammonium sulphate. After the precipitation, separation and resolution of proteins, further purification was performed using the anion-exchange resin Amberlite IRA 958 Cl in a batch process. Partially purified coagulant eluted with 2 mol L-1 NaCl solution achieved the highest coagulation activity of 53.3 % at a dose of 1 mL L-1 although it contained the lowest amount of proteins, but a slightly lower coagulation activity of 49.8 % was achieved at more than 5 times lower dosage of the same fraction. The organic load in treated water when the purified fraction was applied as coagulant was almost 4 times lower than in case of the crude extract as coagulant. |
topic |
proteins coagulation activity water clarification organic load |
url |
http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51392000031P.pdf |
work_keys_str_mv |
AT prodanovicjelenam thepurificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT scibanmarinab thepurificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT kukicdraganav thepurificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT vasicvesnam thepurificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT blagojevnevenat thepurificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT antovmirjanag thepurificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT prodanovicjelenam purificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT scibanmarinab purificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT kukicdraganav purificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT vasicvesnam purificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT blagojevnevenat purificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin AT antovmirjanag purificationofnaturalcoagulantextractedfromcommonbeanonira958clanionexchangeresin |
_version_ |
1724283999592906752 |