Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis

BackgroundFrankliniella occidentalis (Thysanoptera: Thripidae) is a highly rasping-sucking pest of numerous crops. The entomogenous fungi of Lecanicillium spp. are important pathogens of insect pests, and some species have been developed as commercial biopesticides. To explore Lecanicillium spp. res...

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Main Authors: Yeming Zhou, Xiao Zou, Junrui Zhi, Jiqin Xie, Tao Jiang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.561381/full
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spelling doaj-5cdc4c045b674aa9bb31b8b697a87d752020-11-25T03:59:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-10-011110.3389/fmicb.2020.561381561381Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalisYeming Zhou0Xiao Zou1Junrui Zhi2Jiqin Xie3Tao Jiang4Institute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang, ChinaInstitute of Fungus Resources, Guizhou University, Guiyang, ChinaInstitute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang, ChinaInstitute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang, ChinaInstitute of Entomology, Guizhou University, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guiyang, ChinaBackgroundFrankliniella occidentalis (Thysanoptera: Thripidae) is a highly rasping-sucking pest of numerous crops. The entomogenous fungi of Lecanicillium spp. are important pathogens of insect pests, and some species have been developed as commercial biopesticides. To explore Lecanicillium spp. resources in the development of more effective F. occidentalis controls, efficient barcode combinations for strain identification were screened from internal transcribed spacers (ITS), SSU, LSU, TEF, RPB1, and RPB2 genes.ResultsSix genes were used to reconstruct Lecanicillium genus phylogeny. The results showed that ITS, TEF, RPB1, and RPB2 could be used to identify the strains. All phylogenetic trees reconstructed by free combination of these four genes exhibited almost the same topology. Bioassay studies of a purified conidial suspension further confirmed the infection of second-instar nymphs and adult female F. occidentalis by seven Lecanicillium strains. L. attenuatum strain GZUIFR-lun1405 was the most virulent, killing approximately 91.67% F. occidentalis adults and 76.67% nymphs after a 7-day exposure. L. attenuatum strain GZUIFR-lun1405 and L. cauligalbarum strain GZUIFR-ZHJ01 were selected to compare the fungal effects on the number of eggs laid by F. occidentalis. The number of F. occidentalis nymphs significantly decreased when F. occidentalis adults were treated with L. cauligalbarum strain GZUIFR-ZHJ01.ConclusionsThe combination of ITS and RPB1 could be used for fast recognition of Lecanicillium spp. This is the first report of the pathogenicity of L. attenuatum, L. cauligalbarum, L araneogenum, and L. aphanocladii against F. occidentalis. Additionally, L. cauligalbarum strain GZUIFR-ZHJ01 caused high F. occidentalis mortality and inhibited the fecundity of the pest.https://www.frontiersin.org/articles/10.3389/fmicb.2020.561381/fullentomopathogenic fungifast recognitionFrankliniella occidentalisLecanicilliumpathogenicity
collection DOAJ
language English
format Article
sources DOAJ
author Yeming Zhou
Xiao Zou
Junrui Zhi
Jiqin Xie
Tao Jiang
spellingShingle Yeming Zhou
Xiao Zou
Junrui Zhi
Jiqin Xie
Tao Jiang
Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis
Frontiers in Microbiology
entomopathogenic fungi
fast recognition
Frankliniella occidentalis
Lecanicillium
pathogenicity
author_facet Yeming Zhou
Xiao Zou
Junrui Zhi
Jiqin Xie
Tao Jiang
author_sort Yeming Zhou
title Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis
title_short Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis
title_full Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis
title_fullStr Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis
title_full_unstemmed Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis
title_sort fast recognition of lecanicillium spp., and its virulence against frankliniella occidentalis
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2020-10-01
description BackgroundFrankliniella occidentalis (Thysanoptera: Thripidae) is a highly rasping-sucking pest of numerous crops. The entomogenous fungi of Lecanicillium spp. are important pathogens of insect pests, and some species have been developed as commercial biopesticides. To explore Lecanicillium spp. resources in the development of more effective F. occidentalis controls, efficient barcode combinations for strain identification were screened from internal transcribed spacers (ITS), SSU, LSU, TEF, RPB1, and RPB2 genes.ResultsSix genes were used to reconstruct Lecanicillium genus phylogeny. The results showed that ITS, TEF, RPB1, and RPB2 could be used to identify the strains. All phylogenetic trees reconstructed by free combination of these four genes exhibited almost the same topology. Bioassay studies of a purified conidial suspension further confirmed the infection of second-instar nymphs and adult female F. occidentalis by seven Lecanicillium strains. L. attenuatum strain GZUIFR-lun1405 was the most virulent, killing approximately 91.67% F. occidentalis adults and 76.67% nymphs after a 7-day exposure. L. attenuatum strain GZUIFR-lun1405 and L. cauligalbarum strain GZUIFR-ZHJ01 were selected to compare the fungal effects on the number of eggs laid by F. occidentalis. The number of F. occidentalis nymphs significantly decreased when F. occidentalis adults were treated with L. cauligalbarum strain GZUIFR-ZHJ01.ConclusionsThe combination of ITS and RPB1 could be used for fast recognition of Lecanicillium spp. This is the first report of the pathogenicity of L. attenuatum, L. cauligalbarum, L araneogenum, and L. aphanocladii against F. occidentalis. Additionally, L. cauligalbarum strain GZUIFR-ZHJ01 caused high F. occidentalis mortality and inhibited the fecundity of the pest.
topic entomopathogenic fungi
fast recognition
Frankliniella occidentalis
Lecanicillium
pathogenicity
url https://www.frontiersin.org/articles/10.3389/fmicb.2020.561381/full
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