Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model
Conobea scoparioides (Cham. & Schltdl.) Benth. (syn. Sphaerotheca scoparioides Cham. & Schldtl.) (Plantaginaceae), popularly known as ''pataqueira'', ''vassourinha-do-brejo'' and/or “hierba-de-sapo”, is a popular medicinal plant used to treat l...
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Elsevier
2020-09-01
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Series: | Biomedicine & Pharmacotherapy |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332220305953 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emilly J.S.P. de Lima Sheila S. Fontes Mateus L. Nogueira Valdenizia R. Silva Luciano de S. Santos Gigliola M.A. D’Elia Rosane B. Dias Caroline B.S. Sales Clarissa A. Gurgel Rocha Marcos A. Vannier-Santos Milena B.P. Soares Emmanoel V. Costa Felipe M.A. da Silva Hector H.F. Koolen Daniel P. Bezerra |
spellingShingle |
Emilly J.S.P. de Lima Sheila S. Fontes Mateus L. Nogueira Valdenizia R. Silva Luciano de S. Santos Gigliola M.A. D’Elia Rosane B. Dias Caroline B.S. Sales Clarissa A. Gurgel Rocha Marcos A. Vannier-Santos Milena B.P. Soares Emmanoel V. Costa Felipe M.A. da Silva Hector H.F. Koolen Daniel P. Bezerra Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model Biomedicine & Pharmacotherapy Conobea scoparioides Plantaginaceae Anti-liver cancer Apoptosis HepG2 |
author_facet |
Emilly J.S.P. de Lima Sheila S. Fontes Mateus L. Nogueira Valdenizia R. Silva Luciano de S. Santos Gigliola M.A. D’Elia Rosane B. Dias Caroline B.S. Sales Clarissa A. Gurgel Rocha Marcos A. Vannier-Santos Milena B.P. Soares Emmanoel V. Costa Felipe M.A. da Silva Hector H.F. Koolen Daniel P. Bezerra |
author_sort |
Emilly J.S.P. de Lima |
title |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
title_short |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
title_full |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
title_fullStr |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
title_full_unstemmed |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
title_sort |
essential oil from leaves of conobea scoparioides (cham. & schltdl.) benth. (plantaginaceae) causes cell death in hepg2 cells and inhibits tumor development in a xenograft model |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-09-01 |
description |
Conobea scoparioides (Cham. & Schltdl.) Benth. (syn. Sphaerotheca scoparioides Cham. & Schldtl.) (Plantaginaceae), popularly known as ''pataqueira'', ''vassourinha-do-brejo'' and/or “hierba-de-sapo”, is a popular medicinal plant used to treat leishmaniasis, pain and beriberi. In addition, inhibition of cell adhesion, antioxidant, cytotoxic and leishmanicidal activities of compounds or fractions of C. scoparioides have been reported. In the present work, chemical constituents and in vitro and in vivo anti-liver cancer potential of essential oil (EO) from leaves of C. scoparioides were investigated using human hepatocellular carcinoma HepG2 cells as a cell model. EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized by GC–MS and GC–FID. The in vitro cytotoxic effect was evaluated on three human cancer cell lines (MCF-7, HepG2 and HCT116) and one human non-cancerous cell line (MRC-5) using the Alamar blue assay. Phosphatidylserine externalization and cell cycle distribution were quantified in HepG2 cells by flow cytometry after 48 h incubation. The effectiveness of EO in anti-liver cancer model was studied with HepG2 cells grafted on C.B. 17 SCID mice. The main constituents of EO were thymol methyl ether (62 %), thymol (16 %) and α-phellandrene (14 %). EO displayed an in vitro cytotoxic effect against all human cancer cell lines and caused externalization of phosphatidylserine and DNA fragmentation in HepG2 cells, suggesting induction of apoptotic-like cell death. In vivo tumor mass inhibition of 36.7 and 55.8 % was observed for treatment with EO at doses of 40 and 80 mg/kg, respectively. These results indicate in vitro and in vivo anti-liver cancer potential of EO from leaves of C. scoparioides. |
topic |
Conobea scoparioides Plantaginaceae Anti-liver cancer Apoptosis HepG2 |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220305953 |
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doaj-5cf6d412793d4ffbb12d99a66a22c6ec2021-05-20T07:42:39ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-09-01129110402Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft modelEmilly J.S.P. de Lima0Sheila S. Fontes1Mateus L. Nogueira2Valdenizia R. Silva3Luciano de S. Santos4Gigliola M.A. D’Elia5Rosane B. Dias6Caroline B.S. Sales7Clarissa A. Gurgel Rocha8Marcos A. Vannier-Santos9Milena B.P. Soares10Emmanoel V. Costa11Felipe M.A. da Silva12Hector H.F. Koolen13Daniel P. Bezerra14Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus, Amazonas, 690065-130, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, BrazilMetabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus, Amazonas, 690065-130, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, Brazil; Department of Clinical Propaedeutics and Integrated Clinical, Faculty of Dentistry, Federal University of Bahia, Salvador, Bahia, 40301-155, BrazilDepartment of Biomorphology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, 40110-902, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, Brazil; Department of Clinical Propaedeutics and Integrated Clinical, Faculty of Dentistry, Federal University of Bahia, Salvador, Bahia, 40301-155, BrazilOswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, 21040-360, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, BrazilDepartment of Chemistry, Federal University of Amazonas (UFAM), Manaus, Amazonas, 69080-900, BrazilDepartment of Chemistry, Federal University of Amazonas (UFAM), Manaus, Amazonas, 69080-900, BrazilMetabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), Manaus, Amazonas, 690065-130, Brazil; Corresponding authors.Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, Bahia, 40296-710, Brazil; Corresponding authors.Conobea scoparioides (Cham. & Schltdl.) Benth. (syn. Sphaerotheca scoparioides Cham. & Schldtl.) (Plantaginaceae), popularly known as ''pataqueira'', ''vassourinha-do-brejo'' and/or “hierba-de-sapo”, is a popular medicinal plant used to treat leishmaniasis, pain and beriberi. In addition, inhibition of cell adhesion, antioxidant, cytotoxic and leishmanicidal activities of compounds or fractions of C. scoparioides have been reported. In the present work, chemical constituents and in vitro and in vivo anti-liver cancer potential of essential oil (EO) from leaves of C. scoparioides were investigated using human hepatocellular carcinoma HepG2 cells as a cell model. EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized by GC–MS and GC–FID. The in vitro cytotoxic effect was evaluated on three human cancer cell lines (MCF-7, HepG2 and HCT116) and one human non-cancerous cell line (MRC-5) using the Alamar blue assay. Phosphatidylserine externalization and cell cycle distribution were quantified in HepG2 cells by flow cytometry after 48 h incubation. The effectiveness of EO in anti-liver cancer model was studied with HepG2 cells grafted on C.B. 17 SCID mice. The main constituents of EO were thymol methyl ether (62 %), thymol (16 %) and α-phellandrene (14 %). EO displayed an in vitro cytotoxic effect against all human cancer cell lines and caused externalization of phosphatidylserine and DNA fragmentation in HepG2 cells, suggesting induction of apoptotic-like cell death. In vivo tumor mass inhibition of 36.7 and 55.8 % was observed for treatment with EO at doses of 40 and 80 mg/kg, respectively. These results indicate in vitro and in vivo anti-liver cancer potential of EO from leaves of C. scoparioides.http://www.sciencedirect.com/science/article/pii/S0753332220305953Conobea scoparioidesPlantaginaceaeAnti-liver cancerApoptosisHepG2 |