A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I
This paper presents a lateral flow assay (LFA) for the quantitative, fluorescence-based detection of the cardiac biomarker troponin I (cTnI) that features an analytical strip made of cellulose filter paper. The results show that the wicking and test time are comparable to those obtained with convent...
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doaj-5cf93755ba67476bba65a501b6da4a922021-02-15T00:02:06ZengMDPI AGBiosensors2079-63742021-02-0111494910.3390/bios11020049A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin ISatheesh Natarajan0Joseph Jayaraj1Duarte Miguel F. Prazeres2Healthcare Technology Innovation Centre, Indian Institute of Technology, Madras, Chennai, Tamil Nadu 600113, IndiaHealthcare Technology Innovation Centre, Indian Institute of Technology, Madras, Chennai, Tamil Nadu 600113, IndiaIBB—Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, PortugalThis paper presents a lateral flow assay (LFA) for the quantitative, fluorescence-based detection of the cardiac biomarker troponin I (cTnI) that features an analytical strip made of cellulose filter paper. The results show that the wicking and test time are comparable to those obtained with conventional nitrocellulose (NC)-based LFAs. Further, the cellulose paper provides an excellent background with no auto-fluorescence that is very adequate in detecting fluorescent lines. While fluorescence that was generated with cellulose strips was lower when compared to that generated in NC strips, signals could be improved by layering carbon nanofibers (CNF) on the cellulose. A nonlinear behavior of the concentration–response relationship was observed for the LFA architectures with NC, cellulose, and cellulose-CNF in the 0 to 200 ng/mL cTnI concentration range. The measurements were consistent and characterized by coefficients of variation lower than 2.5%. Detection and quantitation limits that were in the range 1.28–1.40 ng/mL and 2.10–2.75 ng/mL were obtained for LFA with cellulose and cellulose CNF strips that are equivalent to the limits obtained with the standard NC LFA. Overall, we showed that commercially available filter paper can be used in the analytical strip of LFA.https://www.mdpi.com/2079-6374/11/2/49biomarkercarbon nanofibercellulosediagnosticsimmunoassaylateral flow assays |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Satheesh Natarajan Joseph Jayaraj Duarte Miguel F. Prazeres |
spellingShingle |
Satheesh Natarajan Joseph Jayaraj Duarte Miguel F. Prazeres A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I Biosensors biomarker carbon nanofiber cellulose diagnostics immunoassay lateral flow assays |
author_facet |
Satheesh Natarajan Joseph Jayaraj Duarte Miguel F. Prazeres |
author_sort |
Satheesh Natarajan |
title |
A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I |
title_short |
A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I |
title_full |
A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I |
title_fullStr |
A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I |
title_full_unstemmed |
A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I |
title_sort |
cellulose paper-based fluorescent lateral flow immunoassay for the quantitative detection of cardiac troponin i |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2021-02-01 |
description |
This paper presents a lateral flow assay (LFA) for the quantitative, fluorescence-based detection of the cardiac biomarker troponin I (cTnI) that features an analytical strip made of cellulose filter paper. The results show that the wicking and test time are comparable to those obtained with conventional nitrocellulose (NC)-based LFAs. Further, the cellulose paper provides an excellent background with no auto-fluorescence that is very adequate in detecting fluorescent lines. While fluorescence that was generated with cellulose strips was lower when compared to that generated in NC strips, signals could be improved by layering carbon nanofibers (CNF) on the cellulose. A nonlinear behavior of the concentration–response relationship was observed for the LFA architectures with NC, cellulose, and cellulose-CNF in the 0 to 200 ng/mL cTnI concentration range. The measurements were consistent and characterized by coefficients of variation lower than 2.5%. Detection and quantitation limits that were in the range 1.28–1.40 ng/mL and 2.10–2.75 ng/mL were obtained for LFA with cellulose and cellulose CNF strips that are equivalent to the limits obtained with the standard NC LFA. Overall, we showed that commercially available filter paper can be used in the analytical strip of LFA. |
topic |
biomarker carbon nanofiber cellulose diagnostics immunoassay lateral flow assays |
url |
https://www.mdpi.com/2079-6374/11/2/49 |
work_keys_str_mv |
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