Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming
Rationale: Little is known about effects of paternal tobacco smoke (PTS) on the offspring’s asthma and its prenatal epigenetic programming.Objective: To investigate whether PTS exposure was associated with the offspring’s asthma and correlated to epigenetic CG methylation of potential tobacco-relate...
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Frontiers Media S.A.
2019-05-01
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Series: | Frontiers in Genetics |
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Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00471/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
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DOAJ |
author |
Chih-Chiang Wu Chih-Chiang Wu Te-Yao Hsu Jen-Chieh Chang Chia-Yu Ou Ho-Chang Kuo Chieh-An Liu Chih-Lu Wang Hau Chuang Chie-Pein Chen Kuender D. Yang Kuender D. Yang Kuender D. Yang Kuender D. Yang |
spellingShingle |
Chih-Chiang Wu Chih-Chiang Wu Te-Yao Hsu Jen-Chieh Chang Chia-Yu Ou Ho-Chang Kuo Chieh-An Liu Chih-Lu Wang Hau Chuang Chie-Pein Chen Kuender D. Yang Kuender D. Yang Kuender D. Yang Kuender D. Yang Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming Frontiers in Genetics paternal tobacco smoke prenatal tobacco smoke exposure asthma development CG methylation LMO2 IL-10 |
author_facet |
Chih-Chiang Wu Chih-Chiang Wu Te-Yao Hsu Jen-Chieh Chang Chia-Yu Ou Ho-Chang Kuo Chieh-An Liu Chih-Lu Wang Hau Chuang Chie-Pein Chen Kuender D. Yang Kuender D. Yang Kuender D. Yang Kuender D. Yang |
author_sort |
Chih-Chiang Wu |
title |
Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming |
title_short |
Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming |
title_full |
Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming |
title_fullStr |
Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming |
title_full_unstemmed |
Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming |
title_sort |
paternal tobacco smoke correlated to offspring asthma and prenatal epigenetic programming |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2019-05-01 |
description |
Rationale: Little is known about effects of paternal tobacco smoke (PTS) on the offspring’s asthma and its prenatal epigenetic programming.Objective: To investigate whether PTS exposure was associated with the offspring’s asthma and correlated to epigenetic CG methylation of potential tobacco-related immune genes: LMO2, GSTM1 or/and IL-10 genes.Measurements and Main Results: In a birth cohort of 1,629 newborns, we measured exposure rates of PTS (23%) and maternal tobacco smoke (MTS, 0.2%), cord blood DNA methylation, infant respiratory tract infection, childhood DNA methylation, and childhood allergic diseases. Infants with prenatal PTS exposure had a significantly higher risk of asthma by the age of 6 than those without (p = 0.026). The PTS exposure doses at 0, <20, and ≧20 cigarettes per day were significantly associated with the trend of childhood asthma and the increase of LMO2-E148 (p = 0.006), and IL10_P325 (p = 0.008) CG methylation. The combination of higher CG methylation levels of LMO2_E148, IL10_P325, and GSTM1_P266 corresponded to the highest risk of asthma by 43.48%, compared to other combinations (16.67–23.08%) in the 3-way multi-factor dimensionality reduction (MDR) analysis. The LMO2_P794 and GSTM1_P266 CG methylation levels at age 0 were significantly correlated to those at age of 6.Conclusions: Prenatal PTS exposure increases CG methylation contents of immune genes, such as LMO2 and IL-10, which significantly retained from newborn stage to 6 years of age and correlated to development of childhood asthma. Modulation of the LMO2 and IL-10 CG methylation and/or their gene expression may provide a regimen for early prevention of PTS-associated childhood asthma.Descriptor number: 1.10 Asthma Mediators.Scientific Knowledge on the Subject: It has been better known that maternal tobacco smoke (MTS) has an impact on the offspring’s asthma via epigenetic modification. Little is known about effects of paternal tobacco smoke (PTS) on the offspring’s asthma and its prenatal epigenetic programming.What This Study Adds to the Field: Prenatal tobacco smoke (PTS) can program epigenetic modifications in certain genes, such as LMO2 and IL-10, and that these modifications are correlated to childhood asthma development. The higher the PTS exposure dose the higher the CG methylation levels are found. The combination of higher CG methylation levels of LMO2_E148, IL10_P325 and GSTM1_P266 corresponded to the highest risk of asthma. Measuring the DNA methylation levels of certain genes might help to predict high-risk populations for childhood asthma and provide a potential target to prevent the development of childhood asthma. |
topic |
paternal tobacco smoke prenatal tobacco smoke exposure asthma development CG methylation LMO2 IL-10 |
url |
https://www.frontiersin.org/article/10.3389/fgene.2019.00471/full |
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doaj-5cfcd5b9c97345cc923b5b6f78edc3922020-11-25T01:27:08ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-05-011010.3389/fgene.2019.00471453403Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic ProgrammingChih-Chiang Wu0Chih-Chiang Wu1Te-Yao Hsu2Jen-Chieh Chang3Chia-Yu Ou4Ho-Chang Kuo5Chieh-An Liu6Chih-Lu Wang7Hau Chuang8Chie-Pein Chen9Kuender D. Yang10Kuender D. Yang11Kuender D. Yang12Kuender D. Yang13Department of Pediatrics, Po-Zen Hospital, Kaohsiung, TaiwanInstitute of Clinical Medicine, National Yang-Ming University, Taipei, TaiwanDepartment of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanGenomic and Proteomic Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanDepartment of Obstetrics, Po-Zen Hospital, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Po-Zen Hospital, Kaohsiung, TaiwanDepartment of Pediatrics, Po-Zen Hospital, Kaohsiung, TaiwanGenomic and Proteomic Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanDepartment of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, TaiwanInstitute of Clinical Medicine, National Yang-Ming University, Taipei, TaiwanDepartment of Pediatrics, Mackay Memorial Hospital, Taipei, TaiwanInstitute of Biomedical Sciences, Mackay Medical College, New Taipei City, Taiwan0Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, TaiwanRationale: Little is known about effects of paternal tobacco smoke (PTS) on the offspring’s asthma and its prenatal epigenetic programming.Objective: To investigate whether PTS exposure was associated with the offspring’s asthma and correlated to epigenetic CG methylation of potential tobacco-related immune genes: LMO2, GSTM1 or/and IL-10 genes.Measurements and Main Results: In a birth cohort of 1,629 newborns, we measured exposure rates of PTS (23%) and maternal tobacco smoke (MTS, 0.2%), cord blood DNA methylation, infant respiratory tract infection, childhood DNA methylation, and childhood allergic diseases. Infants with prenatal PTS exposure had a significantly higher risk of asthma by the age of 6 than those without (p = 0.026). The PTS exposure doses at 0, <20, and ≧20 cigarettes per day were significantly associated with the trend of childhood asthma and the increase of LMO2-E148 (p = 0.006), and IL10_P325 (p = 0.008) CG methylation. The combination of higher CG methylation levels of LMO2_E148, IL10_P325, and GSTM1_P266 corresponded to the highest risk of asthma by 43.48%, compared to other combinations (16.67–23.08%) in the 3-way multi-factor dimensionality reduction (MDR) analysis. The LMO2_P794 and GSTM1_P266 CG methylation levels at age 0 were significantly correlated to those at age of 6.Conclusions: Prenatal PTS exposure increases CG methylation contents of immune genes, such as LMO2 and IL-10, which significantly retained from newborn stage to 6 years of age and correlated to development of childhood asthma. Modulation of the LMO2 and IL-10 CG methylation and/or their gene expression may provide a regimen for early prevention of PTS-associated childhood asthma.Descriptor number: 1.10 Asthma Mediators.Scientific Knowledge on the Subject: It has been better known that maternal tobacco smoke (MTS) has an impact on the offspring’s asthma via epigenetic modification. Little is known about effects of paternal tobacco smoke (PTS) on the offspring’s asthma and its prenatal epigenetic programming.What This Study Adds to the Field: Prenatal tobacco smoke (PTS) can program epigenetic modifications in certain genes, such as LMO2 and IL-10, and that these modifications are correlated to childhood asthma development. The higher the PTS exposure dose the higher the CG methylation levels are found. The combination of higher CG methylation levels of LMO2_E148, IL10_P325 and GSTM1_P266 corresponded to the highest risk of asthma. Measuring the DNA methylation levels of certain genes might help to predict high-risk populations for childhood asthma and provide a potential target to prevent the development of childhood asthma.https://www.frontiersin.org/article/10.3389/fgene.2019.00471/fullpaternal tobacco smokeprenatal tobacco smoke exposureasthma developmentCG methylationLMO2IL-10 |