Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence
Abstract Background The predominant genotype of Toxoplasma in China is the Chinese 1 (ToxoDB#9) lineage. TgCtwh3 and TgCtwh6 are two representative strains of Chinese 1, exhibiting high and low virulence to mice, respectively. Little is known regarding the virulence mechanism of this non-classical g...
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doaj-5d07f26d27394e11b0dcaa6621ca9a742021-05-16T11:09:16ZengBMCParasites & Vectors1756-33052021-05-011411910.1186/s13071-021-04752-zDifferential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulenceYang Wang0Chengjian Han1Rongsheng zhou2Jinjin Zhu3Famin Zhang4Jingyang Li5Qingli Luo6Jian Du7Deyong Chu8Yihong Cai9Jilong Shen10Li Yu11Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityThe Clinical Laboratory of the Third People’s Hospital of HeifeiDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityDepartment of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, School of Basic Medical Sciences, Anhui Medical UniversityAbstract Background The predominant genotype of Toxoplasma in China is the Chinese 1 (ToxoDB#9) lineage. TgCtwh3 and TgCtwh6 are two representative strains of Chinese 1, exhibiting high and low virulence to mice, respectively. Little is known regarding the virulence mechanism of this non-classical genotype. Our previous RNA sequencing data revealed differential mRNA levels of TgMIC1 in TgCtwh3 and TgCtwh6. We aim to further confirm the differential expression of TgMIC1 and its significance in this atypical genotype. Methods Quantitative real-time PCR was used to verify the RNA sequencing data; then, polyclonal antibodies against TgMIC1 were prepared and identified. Moreover, the invasion and proliferation of the parasite in HFF cells were observed after treatment with TgMIC1 polyclonal antibody or not. Results The data showed that the protein level of TgMIC1 was significantly higher in high-virulence strain TgCtwh3 than in low-virulence strain TgCtwh6 and that the invasion and proliferation of TgCtwh3 were inhibited by TgMIC1 polyclonal antibody. Conclusion Differential expression of TgMIC1 in TgCtwh3 and TgCtwh6 may explain, at least partly, the virulence mechanism of this atypical genotype.https://doi.org/10.1186/s13071-021-04752-zToxoplasma gondiiInvasionMicroneme protein 1Polyclonal antibody |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Wang Chengjian Han Rongsheng zhou Jinjin Zhu Famin Zhang Jingyang Li Qingli Luo Jian Du Deyong Chu Yihong Cai Jilong Shen Li Yu |
spellingShingle |
Yang Wang Chengjian Han Rongsheng zhou Jinjin Zhu Famin Zhang Jingyang Li Qingli Luo Jian Du Deyong Chu Yihong Cai Jilong Shen Li Yu Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence Parasites & Vectors Toxoplasma gondii Invasion Microneme protein 1 Polyclonal antibody |
author_facet |
Yang Wang Chengjian Han Rongsheng zhou Jinjin Zhu Famin Zhang Jingyang Li Qingli Luo Jian Du Deyong Chu Yihong Cai Jilong Shen Li Yu |
author_sort |
Yang Wang |
title |
Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence |
title_short |
Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence |
title_full |
Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence |
title_fullStr |
Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence |
title_full_unstemmed |
Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence |
title_sort |
differential expression of tgmic1 in isolates of chinese 1 toxoplasma with different virulence |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2021-05-01 |
description |
Abstract Background The predominant genotype of Toxoplasma in China is the Chinese 1 (ToxoDB#9) lineage. TgCtwh3 and TgCtwh6 are two representative strains of Chinese 1, exhibiting high and low virulence to mice, respectively. Little is known regarding the virulence mechanism of this non-classical genotype. Our previous RNA sequencing data revealed differential mRNA levels of TgMIC1 in TgCtwh3 and TgCtwh6. We aim to further confirm the differential expression of TgMIC1 and its significance in this atypical genotype. Methods Quantitative real-time PCR was used to verify the RNA sequencing data; then, polyclonal antibodies against TgMIC1 were prepared and identified. Moreover, the invasion and proliferation of the parasite in HFF cells were observed after treatment with TgMIC1 polyclonal antibody or not. Results The data showed that the protein level of TgMIC1 was significantly higher in high-virulence strain TgCtwh3 than in low-virulence strain TgCtwh6 and that the invasion and proliferation of TgCtwh3 were inhibited by TgMIC1 polyclonal antibody. Conclusion Differential expression of TgMIC1 in TgCtwh3 and TgCtwh6 may explain, at least partly, the virulence mechanism of this atypical genotype. |
topic |
Toxoplasma gondii Invasion Microneme protein 1 Polyclonal antibody |
url |
https://doi.org/10.1186/s13071-021-04752-z |
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