Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis
Abstract Background When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal eff...
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doaj-5d201bfed66b44d5ac99655d87359dc32020-11-25T01:43:57ZengBMCBMC Infectious Diseases1471-23342017-07-0117111210.1186/s12879-017-2621-4Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitisChih-Hsiang Fang0Pei-I Tsai1Shu-Wei Huang2Jui-Sheng Sun3Jenny Zwei-Chieng Chang4Hsin-Hsin Shen5San-Yuan Chen6Feng Huei Lin7Lih-Tao Hsu8Yen-Chun Chen9Institute of Biomedical Engineering, National Taiwan UniversityDepartment of Materials Science and Engineering, National Chiao-Tung UniversityInstitute of Biomedical Engineering, National Taiwan UniversityDepartment of Orthopedic Surgery, College of Medicine, National Taiwan UniversitySchool of Dentistry, College of Medicine, National Taiwan UniversityTissue Regeneration Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research InstituteDepartment of Materials Science and Engineering, National Chiao-Tung UniversityInstitute of Biomedical Engineering, National Taiwan UniversityIndustrial Technology Research InstituteTissue Regeneration Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research InstituteAbstract Background When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis. Methods Sixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed. Results The pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 °C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control. Conclusions For the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced.http://link.springer.com/article/10.1186/s12879-017-2621-4Peri-implant osteomyelitisMagnetic nanoparticleHyperthermiaBiofilm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chih-Hsiang Fang Pei-I Tsai Shu-Wei Huang Jui-Sheng Sun Jenny Zwei-Chieng Chang Hsin-Hsin Shen San-Yuan Chen Feng Huei Lin Lih-Tao Hsu Yen-Chun Chen |
spellingShingle |
Chih-Hsiang Fang Pei-I Tsai Shu-Wei Huang Jui-Sheng Sun Jenny Zwei-Chieng Chang Hsin-Hsin Shen San-Yuan Chen Feng Huei Lin Lih-Tao Hsu Yen-Chun Chen Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis BMC Infectious Diseases Peri-implant osteomyelitis Magnetic nanoparticle Hyperthermia Biofilm |
author_facet |
Chih-Hsiang Fang Pei-I Tsai Shu-Wei Huang Jui-Sheng Sun Jenny Zwei-Chieng Chang Hsin-Hsin Shen San-Yuan Chen Feng Huei Lin Lih-Tao Hsu Yen-Chun Chen |
author_sort |
Chih-Hsiang Fang |
title |
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
title_short |
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
title_full |
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
title_fullStr |
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
title_full_unstemmed |
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
title_sort |
magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2017-07-01 |
description |
Abstract Background When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis. Methods Sixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed. Results The pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 °C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control. Conclusions For the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced. |
topic |
Peri-implant osteomyelitis Magnetic nanoparticle Hyperthermia Biofilm |
url |
http://link.springer.com/article/10.1186/s12879-017-2621-4 |
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