Genospecies and virulence factors of Aeromonas species in different sources in a North African country
Introduction: Aeromonads of medical importance have been reported from numerous clinical, food, and water sources, but identification of genospecies and virulence factors of Aeromonas species from countries in North Africa and the Middle East are few. Methods: In total 99 Aeromonas species isolates...
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doaj-5d2de2f80bf944248d27b87414595be32020-11-24T23:06:24ZengTaylor & Francis GroupLibyan Journal of Medicine1819-63572014-09-01901510.3402/ljm.v9.2549725497Genospecies and virulence factors of Aeromonas species in different sources in a North African countryKhalifa Sifaw Ghenghesh0Salwa F. Ahmed1Piero Cappuccinelli2John D. Klena3 El-Nakheel Compound, Cairo, Egypt United States Naval Medical Research Unit-3, Cairo, Egypt Department of Biomedical Sciences, Sassari University, Sassari, Italy United States Naval Medical Research Unit-3, Cairo, EgyptIntroduction: Aeromonads of medical importance have been reported from numerous clinical, food, and water sources, but identification of genospecies and virulence factors of Aeromonas species from countries in North Africa and the Middle East are few. Methods: In total 99 Aeromonas species isolates from different sources (diarrheal children [n=23], non-diarrheal children [n=16], untreated drinking water from wells [n=32], and chicken carcasses [n=28]) in Tripoli, Libya, were included in the present investigation. Genus identification was confirmed by biochemical analysis, and genospecies were determined using a combination of 16S rDNA variable region and gyrB sequence analysis. Polymerase chain reaction (PCR) was used to detect genes encoding toxins from 52 of the isolates. Results: We identified 44 isolates (44%) as A. hydrophila (3 [3.0%] subspecies anaerogenes, 23 [23%] subspecies dhakensis, and 18 [18%] subspecies ranae); 27 isolates (27%) as A. veronii; 23 isolates (23%) as A. caviae; and 5 isolates (5.0%) as other genospecies. The genes encoding aerolysin (aer), cytolytic enterotoxin (act), and A. hydrophila isolate SSU enterotoxin (ast) were detected in 45 (87%), 4 (7.7%), and 9 (17%) of the 52 isolates tested, respectively. The gene encoding an extracellular lipase (alt) was not detected. Conclusion: The majority of aeromonads from Libya fall within three genospecies (i.e. A. hydrophila, A. veronii, and A. caviae), and genes coding for toxin production are common among them.http://www.libyanjournalofmedicine.net/index.php/ljm/article/download/25497/pdf_1 Aeromonasgenospeciesvirulence factorsLibya |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khalifa Sifaw Ghenghesh Salwa F. Ahmed Piero Cappuccinelli John D. Klena |
spellingShingle |
Khalifa Sifaw Ghenghesh Salwa F. Ahmed Piero Cappuccinelli John D. Klena Genospecies and virulence factors of Aeromonas species in different sources in a North African country Libyan Journal of Medicine Aeromonas genospecies virulence factors Libya |
author_facet |
Khalifa Sifaw Ghenghesh Salwa F. Ahmed Piero Cappuccinelli John D. Klena |
author_sort |
Khalifa Sifaw Ghenghesh |
title |
Genospecies and virulence factors of Aeromonas species in different sources in a North African country |
title_short |
Genospecies and virulence factors of Aeromonas species in different sources in a North African country |
title_full |
Genospecies and virulence factors of Aeromonas species in different sources in a North African country |
title_fullStr |
Genospecies and virulence factors of Aeromonas species in different sources in a North African country |
title_full_unstemmed |
Genospecies and virulence factors of Aeromonas species in different sources in a North African country |
title_sort |
genospecies and virulence factors of aeromonas species in different sources in a north african country |
publisher |
Taylor & Francis Group |
series |
Libyan Journal of Medicine |
issn |
1819-6357 |
publishDate |
2014-09-01 |
description |
Introduction: Aeromonads of medical importance have been reported from numerous clinical, food, and water sources, but identification of genospecies and virulence factors of Aeromonas species from countries in North Africa and the Middle East are few. Methods: In total 99 Aeromonas species isolates from different sources (diarrheal children [n=23], non-diarrheal children [n=16], untreated drinking water from wells [n=32], and chicken carcasses [n=28]) in Tripoli, Libya, were included in the present investigation. Genus identification was confirmed by biochemical analysis, and genospecies were determined using a combination of 16S rDNA variable region and gyrB sequence analysis. Polymerase chain reaction (PCR) was used to detect genes encoding toxins from 52 of the isolates. Results: We identified 44 isolates (44%) as A. hydrophila (3 [3.0%] subspecies anaerogenes, 23 [23%] subspecies dhakensis, and 18 [18%] subspecies ranae); 27 isolates (27%) as A. veronii; 23 isolates (23%) as A. caviae; and 5 isolates (5.0%) as other genospecies. The genes encoding aerolysin (aer), cytolytic enterotoxin (act), and A. hydrophila isolate SSU enterotoxin (ast) were detected in 45 (87%), 4 (7.7%), and 9 (17%) of the 52 isolates tested, respectively. The gene encoding an extracellular lipase (alt) was not detected. Conclusion: The majority of aeromonads from Libya fall within three genospecies (i.e. A. hydrophila, A. veronii, and A. caviae), and genes coding for toxin production are common among them. |
topic |
Aeromonas genospecies virulence factors Libya |
url |
http://www.libyanjournalofmedicine.net/index.php/ljm/article/download/25497/pdf_1 |
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