An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro
Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to DNA, the origin, type and the passage of transf...
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doaj-5d32c6b5363a42b8ab3e8b8ad45cd77b2021-07-20T15:55:04ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-07-01910.3389/fbioe.2021.701031701031An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitroAgnieszka Fus-Kujawa0Pawel Prus1Pawel Prus2Karolina Bajdak-Rusinek3Paulina Teper4Katarzyna Gawron5Agnieszka Kowalczuk6Aleksander L. Sieron7Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandDepartment of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandStudents’ Scientific Society, Katowice, PolandDepartment of Medical Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze, PolandDepartment of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze, PolandDepartment of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, PolandTransfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to DNA, the origin, type and the passage of transfected cells, and the presence or absence of serum in the cell culture. So far a number of transfection methods that use viruses, non-viral particles or physical factors as the nucleic acids carriers have been developed. Among non-viral carriers, the cationic polymers are proposed as the most attractive ones due to the possibility of their chemical structure modification, low toxicity and immunogenicity. In this review the delivery systems as well as physical, biological and chemical methods used for eukaryotic cells transfection are described and discussed.https://www.frontiersin.org/articles/10.3389/fbioe.2021.701031/fulltransfectioneukaryotic cellspolymersviral carriersendosomal escape |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Agnieszka Fus-Kujawa Pawel Prus Pawel Prus Karolina Bajdak-Rusinek Paulina Teper Katarzyna Gawron Agnieszka Kowalczuk Aleksander L. Sieron |
spellingShingle |
Agnieszka Fus-Kujawa Pawel Prus Pawel Prus Karolina Bajdak-Rusinek Paulina Teper Katarzyna Gawron Agnieszka Kowalczuk Aleksander L. Sieron An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro Frontiers in Bioengineering and Biotechnology transfection eukaryotic cells polymers viral carriers endosomal escape |
author_facet |
Agnieszka Fus-Kujawa Pawel Prus Pawel Prus Karolina Bajdak-Rusinek Paulina Teper Katarzyna Gawron Agnieszka Kowalczuk Aleksander L. Sieron |
author_sort |
Agnieszka Fus-Kujawa |
title |
An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro |
title_short |
An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro |
title_full |
An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro |
title_fullStr |
An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro |
title_full_unstemmed |
An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro |
title_sort |
overview of methods and tools for transfection of eukaryotic cells in vitro |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2021-07-01 |
description |
Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to DNA, the origin, type and the passage of transfected cells, and the presence or absence of serum in the cell culture. So far a number of transfection methods that use viruses, non-viral particles or physical factors as the nucleic acids carriers have been developed. Among non-viral carriers, the cationic polymers are proposed as the most attractive ones due to the possibility of their chemical structure modification, low toxicity and immunogenicity. In this review the delivery systems as well as physical, biological and chemical methods used for eukaryotic cells transfection are described and discussed. |
topic |
transfection eukaryotic cells polymers viral carriers endosomal escape |
url |
https://www.frontiersin.org/articles/10.3389/fbioe.2021.701031/full |
work_keys_str_mv |
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