Two FGFRL-Wnt circuits organize the planarian anteroposterior axis
How positional information instructs adult tissue maintenance is poorly understood. Planarians undergo whole-body regeneration and tissue turnover, providing a model for adult positional information studies. Genes encoding secreted and transmembrane components of multiple developmental pathways are...
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doaj-5d3a17595ed8470ca058878c14d722352021-05-05T00:20:44ZengeLife Sciences Publications LtdeLife2050-084X2016-04-01510.7554/eLife.12845Two FGFRL-Wnt circuits organize the planarian anteroposterior axisM Lucila Scimone0Lauren E Cote1https://orcid.org/0000-0002-1772-7447Travis Rogers2Peter W Reddien3https://orcid.org/0000-0002-5569-333XWhitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United StatesWhitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United StatesWhitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United StatesWhitehead Institute for Biomedical Research, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United StatesHow positional information instructs adult tissue maintenance is poorly understood. Planarians undergo whole-body regeneration and tissue turnover, providing a model for adult positional information studies. Genes encoding secreted and transmembrane components of multiple developmental pathways are predominantly expressed in planarian muscle cells. Several of these genes regulate regional identity, consistent with muscle harboring positional information. Here, single-cell RNA-sequencing of 115 muscle cells from distinct anterior-posterior regions identified 44 regionally expressed genes, including multiple Wnt and ndk/FGF receptor-like (ndl/FGFRL) genes. Two distinct FGFRL-Wnt circuits, involving juxtaposed anterior FGFRL and posterior Wnt expression domains, controlled planarian head and trunk patterning. ndl-3 and wntP-2 inhibition expanded the trunk, forming ectopic mouths and secondary pharynges, which independently extended and ingested food. fz5/8-4 inhibition, like that of ndk and wntA, caused posterior brain expansion and ectopic eye formation. Our results suggest that FGFRL-Wnt circuits operate within a body-wide coordinate system to control adult axial positioning.https://elifesciences.org/articles/12845planarianspatterningWnt signalingFGFRLsingle cellmuscle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M Lucila Scimone Lauren E Cote Travis Rogers Peter W Reddien |
spellingShingle |
M Lucila Scimone Lauren E Cote Travis Rogers Peter W Reddien Two FGFRL-Wnt circuits organize the planarian anteroposterior axis eLife planarians patterning Wnt signaling FGFRL single cell muscle |
author_facet |
M Lucila Scimone Lauren E Cote Travis Rogers Peter W Reddien |
author_sort |
M Lucila Scimone |
title |
Two FGFRL-Wnt circuits organize the planarian anteroposterior axis |
title_short |
Two FGFRL-Wnt circuits organize the planarian anteroposterior axis |
title_full |
Two FGFRL-Wnt circuits organize the planarian anteroposterior axis |
title_fullStr |
Two FGFRL-Wnt circuits organize the planarian anteroposterior axis |
title_full_unstemmed |
Two FGFRL-Wnt circuits organize the planarian anteroposterior axis |
title_sort |
two fgfrl-wnt circuits organize the planarian anteroposterior axis |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2016-04-01 |
description |
How positional information instructs adult tissue maintenance is poorly understood. Planarians undergo whole-body regeneration and tissue turnover, providing a model for adult positional information studies. Genes encoding secreted and transmembrane components of multiple developmental pathways are predominantly expressed in planarian muscle cells. Several of these genes regulate regional identity, consistent with muscle harboring positional information. Here, single-cell RNA-sequencing of 115 muscle cells from distinct anterior-posterior regions identified 44 regionally expressed genes, including multiple Wnt and ndk/FGF receptor-like (ndl/FGFRL) genes. Two distinct FGFRL-Wnt circuits, involving juxtaposed anterior FGFRL and posterior Wnt expression domains, controlled planarian head and trunk patterning. ndl-3 and wntP-2 inhibition expanded the trunk, forming ectopic mouths and secondary pharynges, which independently extended and ingested food. fz5/8-4 inhibition, like that of ndk and wntA, caused posterior brain expansion and ectopic eye formation. Our results suggest that FGFRL-Wnt circuits operate within a body-wide coordinate system to control adult axial positioning. |
topic |
planarians patterning Wnt signaling FGFRL single cell muscle |
url |
https://elifesciences.org/articles/12845 |
work_keys_str_mv |
AT mlucilascimone twofgfrlwntcircuitsorganizetheplanariananteroposterioraxis AT laurenecote twofgfrlwntcircuitsorganizetheplanariananteroposterioraxis AT travisrogers twofgfrlwntcircuitsorganizetheplanariananteroposterioraxis AT peterwreddien twofgfrlwntcircuitsorganizetheplanariananteroposterioraxis |
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1721476382663376896 |