Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2
Background: Sesamin is a lignin present in sesame oil from the bark of <i>Zanthoxylum</i> spp. Sesamin reportedly has anticarcinogenic potential and exerts anti-inflammatory effects on several tumors. Hypothesis/Purpose: However, the effect of sesamin on metastatic progression in human h...
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doaj-5d4280234c7c4fd787ab5be98868df442020-11-25T02:41:49ZengMDPI AGMolecules1420-30492020-05-01252248224810.3390/molecules25092248Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2Jian-Ming Chen0Pei-Yin Chen1Chia-Chieh Lin2Ming-Chang Hsieh3Jen-Tsun Lin4Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, TaiwanDepartment of Recreation and Holistic Wellness, MingDao University, Changhua 523, TaiwanOral Cancer Research Center, Changhua Christian Hospital, Changhua 500, TaiwanSchool of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, TaiwanDivision of Hematology and Oncology, Department of Medicine, Changhua Christian Hospital, Changhua 500, TaiwanBackground: Sesamin is a lignin present in sesame oil from the bark of <i>Zanthoxylum</i> spp. Sesamin reportedly has anticarcinogenic potential and exerts anti-inflammatory effects on several tumors. Hypothesis/Purpose: However, the effect of sesamin on metastatic progression in human head and neck squamous carcinoma (HNSCC) remains unknown in vitro and in vivo; hence, we investigated the effect of sesamin on HNSCC cells in vitro. Methods and Results: Sesamin-treated human oral cancer cell lines FaDu, HSC-3, and Ca9-22 were subjected to a wound-healing assay. Furthermore, Western blotting was performed to assess the effect of sesamin on the expression levels of matrix metalloproteinase (MMP)-2 and proteins of the MAPK signaling pathway, including p-ERK1/2, P-p38, and p-JNK1/2. In addition, we investigated the association between MMP-2 expression and the MAPK pathway in sesamin-treated oral cancer cells. Sesamin inhibited cell migration and invasion in FaDu, Ca9-22, and HSC-3 cells and suppressed MMP-2 at noncytotoxic concentrations (0 to 40 μM). Furthermore, sesamin significantly reduced p38 MAPK and JNK phosphorylation in a dose-dependent manner in FaDu and HSC-3 cells. Conclusions: These results indicate that sesamin suppresses the migration and invasion of HNSCC cells by regulating MMP-2 and is thus a potential antimetastatic agent for treating HNSCC.https://www.mdpi.com/1420-3049/25/9/2248sesaminhuman head and neck squamous carcinomaMMP-2migrationinvasionMAPK |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jian-Ming Chen Pei-Yin Chen Chia-Chieh Lin Ming-Chang Hsieh Jen-Tsun Lin |
spellingShingle |
Jian-Ming Chen Pei-Yin Chen Chia-Chieh Lin Ming-Chang Hsieh Jen-Tsun Lin Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2 Molecules sesamin human head and neck squamous carcinoma MMP-2 migration invasion MAPK |
author_facet |
Jian-Ming Chen Pei-Yin Chen Chia-Chieh Lin Ming-Chang Hsieh Jen-Tsun Lin |
author_sort |
Jian-Ming Chen |
title |
Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2 |
title_short |
Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2 |
title_full |
Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2 |
title_fullStr |
Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2 |
title_full_unstemmed |
Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2 |
title_sort |
antimetastatic effects of sesamin on human head and neck squamous cell carcinoma through regulation of matrix metalloproteinase-2 |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-05-01 |
description |
Background: Sesamin is a lignin present in sesame oil from the bark of <i>Zanthoxylum</i> spp. Sesamin reportedly has anticarcinogenic potential and exerts anti-inflammatory effects on several tumors. Hypothesis/Purpose: However, the effect of sesamin on metastatic progression in human head and neck squamous carcinoma (HNSCC) remains unknown in vitro and in vivo; hence, we investigated the effect of sesamin on HNSCC cells in vitro. Methods and Results: Sesamin-treated human oral cancer cell lines FaDu, HSC-3, and Ca9-22 were subjected to a wound-healing assay. Furthermore, Western blotting was performed to assess the effect of sesamin on the expression levels of matrix metalloproteinase (MMP)-2 and proteins of the MAPK signaling pathway, including p-ERK1/2, P-p38, and p-JNK1/2. In addition, we investigated the association between MMP-2 expression and the MAPK pathway in sesamin-treated oral cancer cells. Sesamin inhibited cell migration and invasion in FaDu, Ca9-22, and HSC-3 cells and suppressed MMP-2 at noncytotoxic concentrations (0 to 40 μM). Furthermore, sesamin significantly reduced p38 MAPK and JNK phosphorylation in a dose-dependent manner in FaDu and HSC-3 cells. Conclusions: These results indicate that sesamin suppresses the migration and invasion of HNSCC cells by regulating MMP-2 and is thus a potential antimetastatic agent for treating HNSCC. |
topic |
sesamin human head and neck squamous carcinoma MMP-2 migration invasion MAPK |
url |
https://www.mdpi.com/1420-3049/25/9/2248 |
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