Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes
Acne is the over growth of the commensal bacteria Propionibacterium acnes (P. acnes) on human skin. Lauric acid (LA) has been investigated as an effective candidate to suppress the activity of P. acnes. Although LA is nearly insoluble in water, dimethyl sulfoxide (DMSO) has been reported to effectiv...
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doaj-5d42a18323f94a53bce2924ce77b6f532020-11-25T00:32:04ZengMDPI AGPolymers2073-43602016-08-018932110.3390/polym8090321polym8090321Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnesThi-Quynh-Mai Tran0Ming-Fa Hsieh1Keng-Lun Chang2Quoc-Hue Pho3Van-Cuong Nguyen4Ching-Yi Cheng5Chun-Ming Huang6International Master Program of Biomedical Material and Technology, Chung Yuan Christian University, 200 Chung-Pei Road, Chung Li District, Taoyuan City 32023, TaiwanInternational Master Program of Biomedical Material and Technology, Chung Yuan Christian University, 200 Chung-Pei Road, Chung Li District, Taoyuan City 32023, TaiwanDepartment of Biomedical Engineering, Chung Yuan Christian University, 200 Chung-Pei Road, Chung Li District, Taoyuan City 32023, TaiwanDepartment of Biomedical Engineering, Chung Yuan Christian University, 200 Chung-Pei Road, Chung Li District, Taoyuan City 32023, TaiwanDepartment of Chemical Engineering, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao St., Go Vap, Ho Chi Minh City 7000, VietnamGraduate Institute of Health Industry Technology and Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanDepartment of Dermatology, School of Medicine, University of California, San Diego, CA 92121, USAAcne is the over growth of the commensal bacteria Propionibacterium acnes (P. acnes) on human skin. Lauric acid (LA) has been investigated as an effective candidate to suppress the activity of P. acnes. Although LA is nearly insoluble in water, dimethyl sulfoxide (DMSO) has been reported to effectively solubilize LA. However, the toxicity of DMSO can limit the use of LA on the skin. In this study, LA-loaded poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) micelles (PCL-PEG-PCL) were developed to improve the bactericidal effect of free LA on P. acnes. The block copolymers mPEG-PCL and PCL-PEG-PCL with different molecular weights were synthesized and characterized using 1H Nuclear Magnetic Resonance spectroscopy (1H NMR), Fourier-transform infrared spectroscopy (FT-IR), Gel Permeation Chromatography (GPC), and Differential Scanning Calorimetry (DSC). In the presence of LA, mPEG-PCL diblock copolymers did not self-assemble into nano-sized micelles. On the contrary, the average particle sizes of the PCL-PEG-PCL micelles ranged from 50–198 nm for blank micelles and 27–89 nm for LA-loaded micelles. The drug loading content increased as the molecular weight of PCL-PEG-PCL polymer increased. Additionally, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of free LA were 20 and 80 μg/mL, respectively. The MICs and MBCs of the micelles decreased to 10 and 40 μg/mL, respectively. This study demonstrated that the LA-loaded micelles are a potential treatment for acne.http://www.mdpi.com/2073-4360/8/9/321poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone)lauric acidmicellesPropionibacterium acnesdrug delivery systemnanoparticles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thi-Quynh-Mai Tran Ming-Fa Hsieh Keng-Lun Chang Quoc-Hue Pho Van-Cuong Nguyen Ching-Yi Cheng Chun-Ming Huang |
spellingShingle |
Thi-Quynh-Mai Tran Ming-Fa Hsieh Keng-Lun Chang Quoc-Hue Pho Van-Cuong Nguyen Ching-Yi Cheng Chun-Ming Huang Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes Polymers poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) lauric acid micelles Propionibacterium acnes drug delivery system nanoparticles |
author_facet |
Thi-Quynh-Mai Tran Ming-Fa Hsieh Keng-Lun Chang Quoc-Hue Pho Van-Cuong Nguyen Ching-Yi Cheng Chun-Ming Huang |
author_sort |
Thi-Quynh-Mai Tran |
title |
Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes |
title_short |
Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes |
title_full |
Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes |
title_fullStr |
Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes |
title_full_unstemmed |
Bactericidal Effect of Lauric Acid-Loaded PCL-PEG-PCL Nano-Sized Micelles on Skin Commensal Propionibacterium acnes |
title_sort |
bactericidal effect of lauric acid-loaded pcl-peg-pcl nano-sized micelles on skin commensal propionibacterium acnes |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2016-08-01 |
description |
Acne is the over growth of the commensal bacteria Propionibacterium acnes (P. acnes) on human skin. Lauric acid (LA) has been investigated as an effective candidate to suppress the activity of P. acnes. Although LA is nearly insoluble in water, dimethyl sulfoxide (DMSO) has been reported to effectively solubilize LA. However, the toxicity of DMSO can limit the use of LA on the skin. In this study, LA-loaded poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) micelles (PCL-PEG-PCL) were developed to improve the bactericidal effect of free LA on P. acnes. The block copolymers mPEG-PCL and PCL-PEG-PCL with different molecular weights were synthesized and characterized using 1H Nuclear Magnetic Resonance spectroscopy (1H NMR), Fourier-transform infrared spectroscopy (FT-IR), Gel Permeation Chromatography (GPC), and Differential Scanning Calorimetry (DSC). In the presence of LA, mPEG-PCL diblock copolymers did not self-assemble into nano-sized micelles. On the contrary, the average particle sizes of the PCL-PEG-PCL micelles ranged from 50–198 nm for blank micelles and 27–89 nm for LA-loaded micelles. The drug loading content increased as the molecular weight of PCL-PEG-PCL polymer increased. Additionally, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of free LA were 20 and 80 μg/mL, respectively. The MICs and MBCs of the micelles decreased to 10 and 40 μg/mL, respectively. This study demonstrated that the LA-loaded micelles are a potential treatment for acne. |
topic |
poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) lauric acid micelles Propionibacterium acnes drug delivery system nanoparticles |
url |
http://www.mdpi.com/2073-4360/8/9/321 |
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