MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell by Regulating Robo1 Expression
Background and objective To explore the function and the potential molecular mechanism of miR-218 in lung cancer cell. Methods The expression of miR-218 mRNA was determined by real-time PCR in lung cancer tissues, adjacent tissues and lung cancer cells. Transwell assay was used to detect the migrati...
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2017-06-01
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Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2017.07.03 |
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doaj-5d4ec9a5be71467cada1f2c7b54862992020-11-24T22:05:24ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872017-06-0120745245810.3779/j.issn.1009-3419.2017.07.03MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell
by Regulating Robo1 ExpressionPing CHEN0Yunlong ZHAO1Yingjie LI2First Affiliated Hospital of PLA General Hospital, Beijing 100048, ChinaFirst Affiliated Hospital of PLA General Hospital, Beijing 100048, ChinaFirst Affiliated Hospital of PLA General Hospital, Beijing 100048, ChinaBackground and objective To explore the function and the potential molecular mechanism of miR-218 in lung cancer cell. Methods The expression of miR-218 mRNA was determined by real-time PCR in lung cancer tissues, adjacent tissues and lung cancer cells. Transwell assay was used to detect the migration and invasion of A549 cell after transfected with Anti-miR-218 or negative control and HC4006 cell after transfected with miR-218 mimics and miR-218 negative control. Targetscan and MiRanda were used to calculate the potential targets of miR-218 and Luciferase reporter assay was performed to identify that the Robo1 was one target genes of miR-218. Transwell assay was used to detect whether miR-218 regulated the invasion of lung cancer cell transfected with anti-miR-218 or negative control via Robo1. Results The expression of miR-218 in the lung cancer tissues was significantly lower than that in the adjacent tissues (P<0.05). Inhibition of miR-218 improved the migration and invasion of A549 cell. Overexpression of miR-218 suppressed the migration and invasion of HCC4006 cell. The co-transfection of anti-miR-218 or miR-218 mimics and the Robo1 3′UTR increased or reduced the luciferase activity of Robo1 compared with the control group (P<0.05). Inhibition of miR-218 and Robo1 recovered the invaded cells of A549. Overexpression of miR-218 and inhibition of Robo1 reduced the number of the invased cells of HCC4006. These results suggested that miR-218 banded Robo1 directly and inhibited lung cancer cell invasion by targeting Robo1. Conclusion MiR-218 inhibited the migration and invasion of lung cancer cells through regulating Robo1 expression.http://dx.doi.org/10.3779/j.issn.1009-3419.2017.07.03Lung neoplasmsmiR-218Robo1MigrationInvasion |
collection |
DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
Ping CHEN Yunlong ZHAO Yingjie LI |
spellingShingle |
Ping CHEN Yunlong ZHAO Yingjie LI MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell by Regulating Robo1 Expression Chinese Journal of Lung Cancer Lung neoplasms miR-218 Robo1 Migration Invasion |
author_facet |
Ping CHEN Yunlong ZHAO Yingjie LI |
author_sort |
Ping CHEN |
title |
MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell
by Regulating Robo1 Expression |
title_short |
MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell
by Regulating Robo1 Expression |
title_full |
MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell
by Regulating Robo1 Expression |
title_fullStr |
MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell
by Regulating Robo1 Expression |
title_full_unstemmed |
MiR-218 Inhibits Migration and Invasion of Lung Cancer Cell
by Regulating Robo1 Expression |
title_sort |
mir-218 inhibits migration and invasion of lung cancer cell
by regulating robo1 expression |
publisher |
Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
series |
Chinese Journal of Lung Cancer |
issn |
1009-3419 1999-6187 |
publishDate |
2017-06-01 |
description |
Background and objective To explore the function and the potential molecular mechanism of miR-218 in lung cancer cell. Methods The expression of miR-218 mRNA was determined by real-time PCR in lung cancer tissues, adjacent tissues and lung cancer cells. Transwell assay was used to detect the migration and invasion of A549 cell after transfected with Anti-miR-218 or negative control and HC4006 cell after transfected with miR-218 mimics and miR-218 negative control. Targetscan and MiRanda were used to calculate the potential targets of miR-218 and Luciferase reporter assay was performed to identify that the Robo1 was one target genes of miR-218. Transwell assay was used to detect whether miR-218 regulated the invasion of lung cancer cell transfected with anti-miR-218 or negative control via Robo1. Results The expression of miR-218 in the lung cancer tissues was significantly lower than that in the adjacent tissues (P<0.05). Inhibition of miR-218 improved the migration and invasion of A549 cell. Overexpression of miR-218 suppressed the migration and invasion of HCC4006 cell. The co-transfection of anti-miR-218 or miR-218 mimics and the Robo1 3′UTR increased or reduced the luciferase activity of Robo1 compared with the control group (P<0.05). Inhibition of miR-218 and Robo1 recovered the invaded cells of A549. Overexpression of miR-218 and inhibition of Robo1 reduced the number of the invased cells of HCC4006. These results suggested that miR-218 banded Robo1 directly and inhibited lung cancer cell invasion by targeting Robo1. Conclusion MiR-218 inhibited the migration and invasion of lung cancer cells through regulating Robo1 expression. |
topic |
Lung neoplasms miR-218 Robo1 Migration Invasion |
url |
http://dx.doi.org/10.3779/j.issn.1009-3419.2017.07.03 |
work_keys_str_mv |
AT pingchen mir218inhibitsmigrationandinvasionoflungcancercellbyregulatingrobo1expression AT yunlongzhao mir218inhibitsmigrationandinvasionoflungcancercellbyregulatingrobo1expression AT yingjieli mir218inhibitsmigrationandinvasionoflungcancercellbyregulatingrobo1expression |
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1725826522605944832 |