Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase)
Extracellular bacterial ribonucleases such as binase from Bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. Moreover, they may induce activation of tumor-derived macrophages either into the M1-phenotype with well-documented functions in the re...
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/2017/4029641 |
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doaj-5d538e4c24d347d1b7f651e4d257fa812020-11-24T20:58:45ZengHindawi LimitedMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/40296414029641Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase)Anna Makeeva0Hector A. Cabrera-Fuentes1Julian Rodriguez-Montesinos2Pavel Zelenikhin3Alexander Nesmelov4Klaus T. Preissner5Olga N. Ilinskaya6Department of Microbiology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, RussiaDepartment of Microbiology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, RussiaInstitute of Biochemistry, Medical School, Justus-Liebig-University, Friedrichstrasse 24, 35390 Giessen, GermanyDepartment of Microbiology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, RussiaDepartment of Microbiology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, RussiaInstitute of Biochemistry, Medical School, Justus-Liebig-University, Friedrichstrasse 24, 35390 Giessen, GermanyDepartment of Microbiology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, RussiaExtracellular bacterial ribonucleases such as binase from Bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. Moreover, they may induce activation of tumor-derived macrophages either into the M1-phenotype with well-documented functions in the regulation of the antitumor immune response or into M2-macrophages that may stimulate tumor growth, metastasis, and angiogenesis. In this study, binase or endogenous RNase1 (but not RNA or short oligonucleotides) stimulated the expression of activated NF-κB p65 subunit in macrophages. Since no changes in MyD88 and TRIF adaptor protein expression were observed, toll-like receptors may not be involved in RNase-related NF-κB pathway activation. In addition, short exposure (0.5 hr) to binase induced the release of cytokines such as IL-6, МСР-1, or TNF-α (but not IL-4 and IL-10), indicative for the polarization into antitumor M1-macrophages. Thus, we revealed increased expression of activated NF-κB p65 subunit in macrophages upon stimulation by binase and RNase1, but not RNA or short oligonucleotides.http://dx.doi.org/10.1155/2017/4029641 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Makeeva Hector A. Cabrera-Fuentes Julian Rodriguez-Montesinos Pavel Zelenikhin Alexander Nesmelov Klaus T. Preissner Olga N. Ilinskaya |
spellingShingle |
Anna Makeeva Hector A. Cabrera-Fuentes Julian Rodriguez-Montesinos Pavel Zelenikhin Alexander Nesmelov Klaus T. Preissner Olga N. Ilinskaya Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase) Mediators of Inflammation |
author_facet |
Anna Makeeva Hector A. Cabrera-Fuentes Julian Rodriguez-Montesinos Pavel Zelenikhin Alexander Nesmelov Klaus T. Preissner Olga N. Ilinskaya |
author_sort |
Anna Makeeva |
title |
Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase) |
title_short |
Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase) |
title_full |
Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase) |
title_fullStr |
Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase) |
title_full_unstemmed |
Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase) |
title_sort |
antitumor macrophage response to bacillus pumilus ribonuclease (binase) |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2017-01-01 |
description |
Extracellular bacterial ribonucleases such as binase from Bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. Moreover, they may induce activation of tumor-derived macrophages either into the M1-phenotype with well-documented functions in the regulation of the antitumor immune response or into M2-macrophages that may stimulate tumor growth, metastasis, and angiogenesis. In this study, binase or endogenous RNase1 (but not RNA or short oligonucleotides) stimulated the expression of activated NF-κB p65 subunit in macrophages. Since no changes in MyD88 and TRIF adaptor protein expression were observed, toll-like receptors may not be involved in RNase-related NF-κB pathway activation. In addition, short exposure (0.5 hr) to binase induced the release of cytokines such as IL-6, МСР-1, or TNF-α (but not IL-4 and IL-10), indicative for the polarization into antitumor M1-macrophages. Thus, we revealed increased expression of activated NF-κB p65 subunit in macrophages upon stimulation by binase and RNase1, but not RNA or short oligonucleotides. |
url |
http://dx.doi.org/10.1155/2017/4029641 |
work_keys_str_mv |
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