CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Abstract Background Circular RNA nuclear factor I X (circNFIX) has been reported to play an important role in glioma progression. However, the mechanism by which circNFIX participates in glioma progression remains poorly understood. Methods GERIA online were used to analyze the abnormally expressed...

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Main Authors: Chenyu Ding, Zanyi Wu, Honghai You, Hongliang Ge, Shufa Zheng, Yuanxiang Lin, Xiyue Wu, Zhangya Lin, Dezhi Kang
Format: Article
Language:English
Published: BMC 2019-12-01
Series:Journal of Experimental & Clinical Cancer Research
Subjects:
Online Access:https://doi.org/10.1186/s13046-019-1483-6
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spelling doaj-5d59167a42024ee9a8839ce8e14ea47c2021-01-03T12:02:56ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662019-12-0138111210.1186/s13046-019-1483-6CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axisChenyu Ding0Zanyi Wu1Honghai You2Hongliang Ge3Shufa Zheng4Yuanxiang Lin5Xiyue Wu6Zhangya Lin7Dezhi Kang8Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityDepartment of Neurosurgery, The First Affiliated Hospital of Fujian Medical UniversityAbstract Background Circular RNA nuclear factor I X (circNFIX) has been reported to play an important role in glioma progression. However, the mechanism by which circNFIX participates in glioma progression remains poorly understood. Methods GERIA online were used to analyze the abnormally expressed genes in glioma tissues. The expression levels of circNFIX, microRNA (miR)-378e and Ribophorin-II (RPN2) were measured by quantitative real-time polymerase chain reaction or western blot. Cell cycle distribution, apoptosis, glycolysis, migration and invasion were determined by flow cytometry, special kit and trans-well assays, respectively. The target association between miR-378e and circNFIX or RPN2 was confirmed by luciferase reporter assay, RNA immunoprecipitation and pull-down. Xenograft model was established to investigate the role of circNFIX in vivo. Results The expression of circNFIX was enhanced in glioma tissues and cells compared with matched controls and high expression of circNFIX indicated poor outcomes of patients. Knockdown of circNFIX led to arrest of cell cycle, inhibition of glycolysis, migration and invasion and promotion of apoptosis in glioma cells. circNFIX was a sponge of miR-378e. miR-378e overexpression suppressed cell cycle process, glycolysis, migration and invasion but promoted apoptosis. miR-378e silence abated the suppressive role of circNFIX knockdown in glioma progression. RPN2 as a target of miR-378e was positively regulated via circNFIX by competitively sponging miR-378e. Silencing circNFIX decreased glioma xenograft tumor growth by regulating miR-378e/RPN2 axis. Conclusion Knockdown of circNFIX inhibits progression of glioma in vitro and in vivo by increasing miR-378e and decreasing RPN2, providing a novel mechanism for understanding the pathogenesis of glioma.https://doi.org/10.1186/s13046-019-1483-6GliomacircNFIXmiR-378eRPN2
collection DOAJ
language English
format Article
sources DOAJ
author Chenyu Ding
Zanyi Wu
Honghai You
Hongliang Ge
Shufa Zheng
Yuanxiang Lin
Xiyue Wu
Zhangya Lin
Dezhi Kang
spellingShingle Chenyu Ding
Zanyi Wu
Honghai You
Hongliang Ge
Shufa Zheng
Yuanxiang Lin
Xiyue Wu
Zhangya Lin
Dezhi Kang
CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis
Journal of Experimental & Clinical Cancer Research
Glioma
circNFIX
miR-378e
RPN2
author_facet Chenyu Ding
Zanyi Wu
Honghai You
Hongliang Ge
Shufa Zheng
Yuanxiang Lin
Xiyue Wu
Zhangya Lin
Dezhi Kang
author_sort Chenyu Ding
title CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis
title_short CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis
title_full CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis
title_fullStr CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis
title_full_unstemmed CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis
title_sort circnfix promotes progression of glioma through regulating mir-378e/rpn2 axis
publisher BMC
series Journal of Experimental & Clinical Cancer Research
issn 1756-9966
publishDate 2019-12-01
description Abstract Background Circular RNA nuclear factor I X (circNFIX) has been reported to play an important role in glioma progression. However, the mechanism by which circNFIX participates in glioma progression remains poorly understood. Methods GERIA online were used to analyze the abnormally expressed genes in glioma tissues. The expression levels of circNFIX, microRNA (miR)-378e and Ribophorin-II (RPN2) were measured by quantitative real-time polymerase chain reaction or western blot. Cell cycle distribution, apoptosis, glycolysis, migration and invasion were determined by flow cytometry, special kit and trans-well assays, respectively. The target association between miR-378e and circNFIX or RPN2 was confirmed by luciferase reporter assay, RNA immunoprecipitation and pull-down. Xenograft model was established to investigate the role of circNFIX in vivo. Results The expression of circNFIX was enhanced in glioma tissues and cells compared with matched controls and high expression of circNFIX indicated poor outcomes of patients. Knockdown of circNFIX led to arrest of cell cycle, inhibition of glycolysis, migration and invasion and promotion of apoptosis in glioma cells. circNFIX was a sponge of miR-378e. miR-378e overexpression suppressed cell cycle process, glycolysis, migration and invasion but promoted apoptosis. miR-378e silence abated the suppressive role of circNFIX knockdown in glioma progression. RPN2 as a target of miR-378e was positively regulated via circNFIX by competitively sponging miR-378e. Silencing circNFIX decreased glioma xenograft tumor growth by regulating miR-378e/RPN2 axis. Conclusion Knockdown of circNFIX inhibits progression of glioma in vitro and in vivo by increasing miR-378e and decreasing RPN2, providing a novel mechanism for understanding the pathogenesis of glioma.
topic Glioma
circNFIX
miR-378e
RPN2
url https://doi.org/10.1186/s13046-019-1483-6
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