Optimization studies on biodegradation of atrazine by Bacillus badius ABP6 strain using response surface methodology

• Main Authors: Hina Khatoon, J.P.N. Rai
• Format: Article
• Language: English
• Published: Elsevier 2020-06-01
• Series: Biotechnology Reports
• Subjects: Atrazine; Bacillus badius ABP6 strain; Biodegradation; Optimization; Response-surface-methodology (RSM)
• Online Access: http://www.sciencedirect.com/science/article/pii/S2215017X19305776

In this study, the optimization of distinctive environmental factors such as pH, temperature, agitation-speed and atrazine-concentration on atrazine degradation by utilizing Bacillus badius ABP6 strain, has been done through response-surface-methodology (RSM). The optimum-conditions after analysis for the maximum atrazine degradation were: pH 7.05, temperature 30.4 °C, agitation-speed 145.7 rpm, and atrazine-concentration 200.9 ppm. The prescribed model was approved for high F-value (95.92), very low P-value (<0.01) and non- significant lack of fit (0.1627). It was observed that under the optimized-conditions, the R2 value of regression models for all the response variables was 0.9897 and the maximum atrazine degradation i.e. 89.7 % was found. Finally for graphical representation, the validated optimum-conditions of variables and responses were simulated using three dimensional plots (3D). The confirmation of the model is successful to suggest the optimization parameters of atrazine degradation under in-situ condition by bacterial isolate employing response-surface-methodology optimization tool of Design expert software (new version 10.0.1).