CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer
<p>Abstract</p> <p>Background</p> <p>Gene silencing associated with aberrant methylation of promoter region CpG islands is an acquired epigenetic alteration that serves as an alternative to genetic defects in the inactivation of tumor suppressor and other genes in human...
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doaj-5d6d596b5ac945489dd87dd658fab4772020-11-24T21:23:49ZengBMCEuropean Journal of Medical Research2047-783X2009-02-011427110.1186/2047-783X-14-2-71CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancerTamandani DMSobti RCShekari MHuria A<p>Abstract</p> <p>Background</p> <p>Gene silencing associated with aberrant methylation of promoter region CpG islands is an acquired epigenetic alteration that serves as an alternative to genetic defects in the inactivation of tumor suppressor and other genes in human cancers.</p> <p>Aims</p> <p>This study describes the methylation status of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer. We also examined the prevalence of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes methylation in cervical cancer tissue and none - neo plastic samples in an effort to correlate with smoking habit and clinicopathological features.</p> <p>Method</p> <p>Target DNA was modified by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequently amplified by Methylation Specific (MS) PCR with primers specific for methylated versus unmethylated DNA. The PCR product was detected by gel electrophoresis and combined with the clinical records of patients.</p> <p>Results</p> <p>The methylation pattern of the <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in specimens of cervical cancer and adjacent normal tissues were detected [5/80 (6.2%), 3/80 (3.75%)-2/80 (2.5%), 1/80 (1.2%) respectively]. No statistical differences were seen in the extent of differentiation, invasion, pathological type and smoking habit between the methylated and unmethylated tissues (<it>P </it>> 0.05).</p> <p>Conclusion</p> <p>The present study conclude that the frequency of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes methylation in cervical cancer are rare (< 6%), and have no any critical role in development of cervical cancer.</p> http://www.eurjmedres.com/content/14/2/71Methylation<it>TMS1</it>/<it>ASC</it><it>CASP8</it>cervical cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tamandani DM Sobti RC Shekari M Huria A |
spellingShingle |
Tamandani DM Sobti RC Shekari M Huria A CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer European Journal of Medical Research Methylation <it>TMS1</it>/<it>ASC</it> <it>CASP8</it> cervical cancer |
author_facet |
Tamandani DM Sobti RC Shekari M Huria A |
author_sort |
Tamandani DM |
title |
CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer |
title_short |
CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer |
title_full |
CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer |
title_fullStr |
CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer |
title_full_unstemmed |
CpG island methylation of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer |
title_sort |
cpg island methylation of <it>tms1</it>/<it>asc </it>and <it>casp8 </it>genes in cervical cancer |
publisher |
BMC |
series |
European Journal of Medical Research |
issn |
2047-783X |
publishDate |
2009-02-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Gene silencing associated with aberrant methylation of promoter region CpG islands is an acquired epigenetic alteration that serves as an alternative to genetic defects in the inactivation of tumor suppressor and other genes in human cancers.</p> <p>Aims</p> <p>This study describes the methylation status of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in cervical cancer. We also examined the prevalence of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes methylation in cervical cancer tissue and none - neo plastic samples in an effort to correlate with smoking habit and clinicopathological features.</p> <p>Method</p> <p>Target DNA was modified by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequently amplified by Methylation Specific (MS) PCR with primers specific for methylated versus unmethylated DNA. The PCR product was detected by gel electrophoresis and combined with the clinical records of patients.</p> <p>Results</p> <p>The methylation pattern of the <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes in specimens of cervical cancer and adjacent normal tissues were detected [5/80 (6.2%), 3/80 (3.75%)-2/80 (2.5%), 1/80 (1.2%) respectively]. No statistical differences were seen in the extent of differentiation, invasion, pathological type and smoking habit between the methylated and unmethylated tissues (<it>P </it>> 0.05).</p> <p>Conclusion</p> <p>The present study conclude that the frequency of <it>TMS1</it>/<it>ASC </it>and <it>CASP8 </it>genes methylation in cervical cancer are rare (< 6%), and have no any critical role in development of cervical cancer.</p> |
topic |
Methylation <it>TMS1</it>/<it>ASC</it> <it>CASP8</it> cervical cancer |
url |
http://www.eurjmedres.com/content/14/2/71 |
work_keys_str_mv |
AT tamandanidm cpgislandmethylationofittms1ititascitanditcasp8itgenesincervicalcancer AT sobtirc cpgislandmethylationofittms1ititascitanditcasp8itgenesincervicalcancer AT shekarim cpgislandmethylationofittms1ititascitanditcasp8itgenesincervicalcancer AT huriaa cpgislandmethylationofittms1ititascitanditcasp8itgenesincervicalcancer |
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