miR-424-3p Contributes to the Malignant Progression and Chemoresistance of Gastric Cancer

• Main Authors: Li Y, Liu H, Cui Y, Chen H, Cui X, Shao J, Su F, He X
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-11-01
• Series: OncoTargets and Therapy
• Subjects: gastric cancer; mir-424-3p; chemoresistance; malignant progression
• Online Access: https://www.dovepress.com/mir-424-3p-contributes-to-the-malignant-progression-and-chemoresistanc-peer-reviewed-article-OTT

Yongyuan Li,1,2,* Hongjie Liu,3,* Yu Cui,1 Hekai Chen,1 Xuejun Cui,1 Jianping Shao,1 Feng Su,1 Xianghui He2 1Department of General Surgery, The Fifth Central Hospital, Tianjin 300450, People’s Republic of China; 2Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, People’s Republic of China; 3Department of Radiology, The Fifth Central Hospital, Tianjin 300450, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xianghui HeDepartment of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, People’s Republic of ChinaEmail [e-mail]: Gastric cancer (GC) is one of the most common and lethal malignancies worldwide. Therefore, a better understanding of the mechanism of its malignant progression and chemoresistance will be helpful for the treatment of patients with GC.Methods: The gene expression profiles downloaded from GEO database and the TargetScan Human were used to identify the key regulation model based on miRNA by bioinformatics analyses. The regulation of miRNA to target was clarified by luciferase assay, qPCR, and Western blotting. Then, the in vitro and in vivo experiments were further conducted by overexpression or knockdown of miRNA and/or target to examine the regulation effects and clarify the mechanism.Results: In the present study, miR-424-3p was identified to be differentially expressed among normal gastric, GC, and chemoresistant GC tissues. Target analysis results indicated that ABCC2, a chemoresistance-related gene, was a regulated target of miR-424-3p. The in vitro and in vivo experiment results further demonstrated that miR-424-3p relied on ABCC2-induced chemoresistance to promote GC proliferation and metastasis.Conclusion: Overall, this study revealed that miR-424-3p contributed to the malignant progression and chemoresistance of GC. Thus, miR-424-3p could be a potential target for the treatment of GC.Keywords: gastric cancer, miR-424-3p, chemoresistance, malignant progression