GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally c...
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doaj-5d7600d7b4f04b6482212f9e3e8bd2532020-11-25T00:54:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-06-01910.3389/fpls.2018.00809317415GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic ArabidopsisZhaolong Xu0Qasim Raza1Qasim Raza2Ling Xu3Xiaolan He4Yihong Huang5Jinxin Yi6Dayong Zhang7Hong-Bo Shao8Hong-Bo Shao9Hongxiang Ma10Zulfiqar Ali11Salt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Plant Breeding and Genetics, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanRice Research Institute, Kala Shah Kaku, PakistanSalt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSalt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSalt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSalt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSalt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSalt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaJiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University, Yancheng, ChinaInstitute of Grain Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaDepartment of Plant Breeding and Genetics, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanPlant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C2H2-type zinc-finger structure. The normalized expression (log2 ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement.https://www.frontiersin.org/article/10.3389/fpls.2018.00809/fullGlycine maxGmWRKY49overexpressionsalinity toleranceWRKY TFs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhaolong Xu Qasim Raza Qasim Raza Ling Xu Xiaolan He Yihong Huang Jinxin Yi Dayong Zhang Hong-Bo Shao Hong-Bo Shao Hongxiang Ma Zulfiqar Ali |
spellingShingle |
Zhaolong Xu Qasim Raza Qasim Raza Ling Xu Xiaolan He Yihong Huang Jinxin Yi Dayong Zhang Hong-Bo Shao Hong-Bo Shao Hongxiang Ma Zulfiqar Ali GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis Frontiers in Plant Science Glycine max GmWRKY49 overexpression salinity tolerance WRKY TFs |
author_facet |
Zhaolong Xu Qasim Raza Qasim Raza Ling Xu Xiaolan He Yihong Huang Jinxin Yi Dayong Zhang Hong-Bo Shao Hong-Bo Shao Hongxiang Ma Zulfiqar Ali |
author_sort |
Zhaolong Xu |
title |
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis |
title_short |
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis |
title_full |
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis |
title_fullStr |
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis |
title_full_unstemmed |
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis |
title_sort |
gmwrky49, a salt-responsive nuclear protein, improved root length and governed better salinity tolerance in transgenic arabidopsis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2018-06-01 |
description |
Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C2H2-type zinc-finger structure. The normalized expression (log2 ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. |
topic |
Glycine max GmWRKY49 overexpression salinity tolerance WRKY TFs |
url |
https://www.frontiersin.org/article/10.3389/fpls.2018.00809/full |
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