Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China
Abstract Background Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes...
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BMC
2021-07-01
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Series: | Parasites & Vectors |
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Online Access: | https://doi.org/10.1186/s13071-021-04809-z |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaowa Wulantuya Kozue Sato Dan Liu Yunhong Cui Xuhong Yin Lihua Zhang Hong Li Tingfu Wang Rongxin Liu Lijing Wu Saixia Lu Ting Gao Zitong Zhang Minzhi Cao Guodong Wang Chunpu Li Dacheng Yan Norio Ohashi Shuji Ando Hiroki Kawabata |
spellingShingle |
Gaowa Wulantuya Kozue Sato Dan Liu Yunhong Cui Xuhong Yin Lihua Zhang Hong Li Tingfu Wang Rongxin Liu Lijing Wu Saixia Lu Ting Gao Zitong Zhang Minzhi Cao Guodong Wang Chunpu Li Dacheng Yan Norio Ohashi Shuji Ando Hiroki Kawabata Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China Parasites & Vectors Ixodes persulcatus Borrelia miyamotoi MLSA Inner Mongolia |
author_facet |
Gaowa Wulantuya Kozue Sato Dan Liu Yunhong Cui Xuhong Yin Lihua Zhang Hong Li Tingfu Wang Rongxin Liu Lijing Wu Saixia Lu Ting Gao Zitong Zhang Minzhi Cao Guodong Wang Chunpu Li Dacheng Yan Norio Ohashi Shuji Ando Hiroki Kawabata |
author_sort |
Gaowa |
title |
Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China |
title_short |
Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China |
title_full |
Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China |
title_fullStr |
Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China |
title_full_unstemmed |
Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China |
title_sort |
surveillance of borrelia miyamotoi-carrying ticks and genomic analysis of isolates in inner mongolia, china |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2021-07-01 |
description |
Abstract Background Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China. Methods From May to June each year during the period 2016–2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA). Results A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia. Conclusion We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia. |
topic |
Ixodes persulcatus Borrelia miyamotoi MLSA Inner Mongolia |
url |
https://doi.org/10.1186/s13071-021-04809-z |
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doaj-5dc2158d4fba4331b5ec2bdeebf0685d2021-07-18T11:29:45ZengBMCParasites & Vectors1756-33052021-07-011411810.1186/s13071-021-04809-zSurveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, ChinaGaowa0Wulantuya1Kozue Sato2Dan Liu3Yunhong Cui4Xuhong Yin5Lihua Zhang6Hong Li7Tingfu Wang8Rongxin Liu9Lijing Wu10Saixia Lu11Ting Gao12Zitong Zhang13Minzhi Cao14Guodong Wang15Chunpu Li16Dacheng Yan17Norio Ohashi18Shuji Ando19Hiroki Kawabata20Inner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoDepartment of Bacteriology-I, National Institute of Infectious DiseasesInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoInner Mongolia Key Laboratory of Tick-Borne Zoonotic Infectious Disease, Department of Medicine, College of HetaoBayan Nur Centers for Disease Control and PreventionHulunbuir Centers for Disease Control and PreventionHulunbuir Centers for Disease Control and PreventionHulunbuir Centers for Disease Control and PreventionLaboratory of Microbiology, Department of Food and Nutritional Sciences, University of ShizuokaDepartment of Virology, National Institute of Infectious DiseasesDepartment of Bacteriology-I, National Institute of Infectious DiseasesAbstract Background Borrelia miyamotoi is a newly described relapsing fever spirochete transmitted by ixodid tick species. Little is known about the prevalence of B. miyamotoi infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of B. miyamotoi in Ixodes persulcatus ticks, and we aimed to isolateB. miyamotoi from I. persulcatus from four regions of Greater Khingan, Inner Mongolia, China. Methods From May to June each year during the period 2016–2019, host-seeking adult I. persulcatus ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate B. miyamotoi in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect Borrelia DNA in the ticks and to calculate the prevalence of B. miyamotoi and infections with other borreliae. For characterization of the isolated B. miyamotoi, we performed draft genome sequencing and multilocus sequencing analysis (MLSA). Results A total of 2656 adult I. persulcatus ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (glpQ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as B. miyamotoi. Furthermore, the B. miyamotoi strain Hetao-1 was isolated from I. persulcatus, and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1 kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia. Conclusion We detected B. miyamotoi from I. persulcatus ticks collected in Inner Mongolia, and successfully isolated a B. miyamotoi strain. To our knowledge, this is the first study to culture a B. miyamotoi isolate from China. The data on the prevalence of B. miyamotoi and other borreliae in I. persulcatus ticks will be fundamental for future epidemiological studies of B. miyamotoi disease in Inner Mongolia.https://doi.org/10.1186/s13071-021-04809-zIxodes persulcatusBorrelia miyamotoiMLSAInner Mongolia |