PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin

PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin

Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be deve...

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Main Authors: Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K. Kaneko, Yukinari Kato
Format: Article
Language:English
Published: Elsevier 2019-07-01
Series:Biochemistry and Biophysics Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580818302681
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spelling doaj-5de703a97af24660a1b1fd7587fd4e0a2020-11-25T01:57:46ZengElsevierBiochemistry and Biophysics Reports2405-58082019-07-0118PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplaninYoshikazu Furusawa0Shinji Yamada1Shunsuke Itai2Takuro Nakamura3Miyuki Yanaka4Masato Sano5Hiroyuki Harada6Masato Fukui7Mika K. Kaneko8Yukinari Kato9Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; New Industry Creation Hatchery Center, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; ZENOAQ RESOURCE CO., LTD, 1-1 Tairanoue, Sasagawa, Asaka-machi, Koriyama, Fukushima, 963-0196, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, JapanZENOAQ RESOURCE CO., LTD, 1-1 Tairanoue, Sasagawa, Asaka-machi, Koriyama, Fukushima, 963-0196, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; New Industry Creation Hatchery Center, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; Corresponding author.New Industry Creation Hatchery Center, Tohoku University, 2-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan.Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG2a, kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting. Furthermore, PMab-219 strongly stained CHO/horPDPN via immunohistochemistry. These findings suggest that PMab-219 is useful for investigating the function of horPDPN. Keywords: Horse podoplanin, PDPN, PMab-219http://www.sciencedirect.com/science/article/pii/S2405580818302681
collection DOAJ
language English
format Article
sources DOAJ
author Yoshikazu Furusawa
Shinji Yamada
Shunsuke Itai
Takuro Nakamura
Miyuki Yanaka
Masato Sano
Hiroyuki Harada
Masato Fukui
Mika K. Kaneko
Yukinari Kato
spellingShingle Yoshikazu Furusawa
Shinji Yamada
Shunsuke Itai
Takuro Nakamura
Miyuki Yanaka
Masato Sano
Hiroyuki Harada
Masato Fukui
Mika K. Kaneko
Yukinari Kato
PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
Biochemistry and Biophysics Reports
author_facet Yoshikazu Furusawa
Shinji Yamada
Shunsuke Itai
Takuro Nakamura
Miyuki Yanaka
Masato Sano
Hiroyuki Harada
Masato Fukui
Mika K. Kaneko
Yukinari Kato
author_sort Yoshikazu Furusawa
title PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_short PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_full PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_fullStr PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_full_unstemmed PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin
title_sort pmab-219: a monoclonal antibody for the immunohistochemical analysis of horse podoplanin
publisher Elsevier
series Biochemistry and Biophysics Reports
issn 2405-5808
publishDate 2019-07-01
description Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG2a, kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting. Furthermore, PMab-219 strongly stained CHO/horPDPN via immunohistochemistry. These findings suggest that PMab-219 is useful for investigating the function of horPDPN. Keywords: Horse podoplanin, PDPN, PMab-219
url http://www.sciencedirect.com/science/article/pii/S2405580818302681
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