Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis
The origin of crescent forming cells in human glomerulonephritis (GN) remains unknown. Some animal studies demonstrated that parietal epithelial cells of Bowman’s capsule (PECs) were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescent...
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Series: | International Journal of Nephrology |
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doaj-5dfa8448cf2e41fca1176334b69f42d92020-11-24T22:09:34ZengHindawi LimitedInternational Journal of Nephrology2090-214X2090-21582014-01-01201410.1155/2014/598670598670Expression of Tight Junction Protein Claudin-1 in Human Crescentic GlomerulonephritisRyo Koda0Atsunori Yoshino1Yuji Imanishi2Shinya Kawamoto3Yoshihiko Ueda4Eishin Yaoita5Junichiro James Kazama6Ichiei Narita7Tetsuro Takeda8Department of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, JapanDepartment of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, JapanDepartment of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, JapanDepartment of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, JapanDepartment of Pathology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, JapanDepartment of Structural Pathology, Institute of Nephrology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Niigata 951-8510, JapanDivision of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Niigata 951-8510, JapanDivision of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Niigata 951-8510, JapanDepartment of Nephrology, Dokkyo Medical University Koshigaya Hospital, No. 1-50, 2-Chome, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, JapanThe origin of crescent forming cells in human glomerulonephritis (GN) remains unknown. Some animal studies demonstrated that parietal epithelial cells of Bowman’s capsule (PECs) were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescentic lesions. We investigated the expression of claudin-1 in human GN. Immunohistochemistry for claudin-1 was performed on 17 kidney biopsy samples with crescent formation. Colocalization of claudin-1 with intracellular tight junction protein ZO-1 was also evaluated by immunofluorescence double staining. Claudin-1 is expressed mainly at the cell to cell contact site of proliferating cells in cellular crescentic lesions in patients with these forms of human GN. Small numbers of crescent forming cells showed extrajunctional localization of claudin-1. Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells. In control samples, staining of claudin-1 was positive in PECs, but not in podocytes. Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1. Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman’s space.http://dx.doi.org/10.1155/2014/598670 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryo Koda Atsunori Yoshino Yuji Imanishi Shinya Kawamoto Yoshihiko Ueda Eishin Yaoita Junichiro James Kazama Ichiei Narita Tetsuro Takeda |
spellingShingle |
Ryo Koda Atsunori Yoshino Yuji Imanishi Shinya Kawamoto Yoshihiko Ueda Eishin Yaoita Junichiro James Kazama Ichiei Narita Tetsuro Takeda Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis International Journal of Nephrology |
author_facet |
Ryo Koda Atsunori Yoshino Yuji Imanishi Shinya Kawamoto Yoshihiko Ueda Eishin Yaoita Junichiro James Kazama Ichiei Narita Tetsuro Takeda |
author_sort |
Ryo Koda |
title |
Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis |
title_short |
Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis |
title_full |
Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis |
title_fullStr |
Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis |
title_full_unstemmed |
Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis |
title_sort |
expression of tight junction protein claudin-1 in human crescentic glomerulonephritis |
publisher |
Hindawi Limited |
series |
International Journal of Nephrology |
issn |
2090-214X 2090-2158 |
publishDate |
2014-01-01 |
description |
The origin of crescent forming cells in human glomerulonephritis (GN) remains unknown. Some animal studies demonstrated that parietal epithelial cells of Bowman’s capsule (PECs) were the main component of proliferating cells and PEC-specific tight junction protein claudin-1 was expressed in crescentic lesions. We investigated the expression of claudin-1 in human GN. Immunohistochemistry for claudin-1 was performed on 17 kidney biopsy samples with crescent formation. Colocalization of claudin-1 with intracellular tight junction protein ZO-1 was also evaluated by immunofluorescence double staining. Claudin-1 is expressed mainly at the cell to cell contact site of proliferating cells in cellular crescentic lesions in patients with these forms of human GN. Small numbers of crescent forming cells showed extrajunctional localization of claudin-1. Colocalization of claudin-1 with ZO-1 was found at cell to cell contact sites of adjacent proliferating cells. In control samples, staining of claudin-1 was positive in PECs, but not in podocytes. Our findings suggest that claudin-1 contributes to crescent formation as a component of the tight junction protein complex that includes ZO-1. Co-localization of claudin-1 with ZO-1 implies the formation of functional tight junction complexes in crescentic lesions to prevent the interstitial damage caused by penetration of filtered molecules from Bowman’s space. |
url |
http://dx.doi.org/10.1155/2014/598670 |
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