Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection

Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection

Proteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue s...

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Main Authors: Dinusha N. Udukala, Hongwang Wang, Sebastian O. Wendel, Aruni P. Malalasekera, Thilani N. Samarakoon, Asanka S. Yapa, Gayani Abayaweera, Matthew T. Basel, Pamela Maynez, Raquel Ortega, Yubisela Toledo, Leonie Bossmann, Colette Robinson, Katharine E. Janik, Olga B. Koper, Ping Li, Massoud Motamedi, Daniel A. Higgins, Gary Gadbury, Gaohong Zhu, Deryl L. Troyer, Stefan H. Bossmann
Format: Article
Language:English
Published: Beilstein-Institut 2016-03-01
Series:Beilstein Journal of Nanotechnology
Subjects:
biophotonics
breast cancer
iron/iron oxide nanoparticle
liquid biopsy
nanodiagnostics detection
nanomedicine
sub-femtomolar limit of detection
Online Access:https://doi.org/10.3762/bjnano.7.33
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spelling doaj-5dfb1aeb2f4f489e9b23ff2bd73239e22020-11-25T01:00:11ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862016-03-017136437310.3762/bjnano.7.332190-4286-7-33Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detectionDinusha N. Udukala0Hongwang Wang1Sebastian O. Wendel2Aruni P. Malalasekera3Thilani N. Samarakoon4Asanka S. Yapa5Gayani Abayaweera6Matthew T. Basel7Pamela Maynez8Raquel Ortega9Yubisela Toledo10Leonie Bossmann11Colette Robinson12Katharine E. Janik13Olga B. Koper14Ping Li15Massoud Motamedi16Daniel A. Higgins17Gary Gadbury18Gaohong Zhu19Deryl L. Troyer20Stefan H. Bossmann21Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Anatomy & Physiology, 228 Coles Hall, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAThe University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Statistics, 101 Dickens Hall, Manhattan, KS, USAThe First Affiliated Hospital of Kunming Medical University, Department of Nuclear Medicine, 295 Xichang Road, Kunming, Yunnan, PR ChinaKansas State University, Department of Anatomy & Physiology, 228 Coles Hall, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAProteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue samples and liquid biopsies is a vital strategy for early cancer detection. Water-dispersable Fe/Fe3O4-core/shell based nanoplatforms for protease detection are capable of detecting protease activity down to sub-femtomolar limits of detection. They feature one dye (tetrakis(carboxyphenyl)porphyrin (TCPP)) that is tethered to the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis.https://doi.org/10.3762/bjnano.7.33biophotonicsbreast canceriron/iron oxide nanoparticleliquid biopsynanodiagnostics detectionnanomedicinesub-femtomolar limit of detection
collection DOAJ
language English
format Article
sources DOAJ
author Dinusha N. Udukala
Hongwang Wang
Sebastian O. Wendel
Aruni P. Malalasekera
Thilani N. Samarakoon
Asanka S. Yapa
Gayani Abayaweera
Matthew T. Basel
Pamela Maynez
Raquel Ortega
Yubisela Toledo
Leonie Bossmann
Colette Robinson
Katharine E. Janik
Olga B. Koper
Ping Li
Massoud Motamedi
Daniel A. Higgins
Gary Gadbury
Gaohong Zhu
Deryl L. Troyer
Stefan H. Bossmann
spellingShingle Dinusha N. Udukala
Hongwang Wang
Sebastian O. Wendel
Aruni P. Malalasekera
Thilani N. Samarakoon
Asanka S. Yapa
Gayani Abayaweera
Matthew T. Basel
Pamela Maynez
Raquel Ortega
Yubisela Toledo
Leonie Bossmann
Colette Robinson
Katharine E. Janik
Olga B. Koper
Ping Li
Massoud Motamedi
Daniel A. Higgins
Gary Gadbury
Gaohong Zhu
Deryl L. Troyer
Stefan H. Bossmann
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
Beilstein Journal of Nanotechnology
biophotonics
breast cancer
iron/iron oxide nanoparticle
liquid biopsy
nanodiagnostics detection
nanomedicine
sub-femtomolar limit of detection
author_facet Dinusha N. Udukala
Hongwang Wang
Sebastian O. Wendel
Aruni P. Malalasekera
Thilani N. Samarakoon
Asanka S. Yapa
Gayani Abayaweera
Matthew T. Basel
Pamela Maynez
Raquel Ortega
Yubisela Toledo
Leonie Bossmann
Colette Robinson
Katharine E. Janik
Olga B. Koper
Ping Li
Massoud Motamedi
Daniel A. Higgins
Gary Gadbury
Gaohong Zhu
Deryl L. Troyer
Stefan H. Bossmann
author_sort Dinusha N. Udukala
title Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
title_short Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
title_full Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
title_fullStr Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
title_full_unstemmed Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
title_sort early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
publisher Beilstein-Institut
series Beilstein Journal of Nanotechnology
issn 2190-4286
publishDate 2016-03-01
description Proteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue samples and liquid biopsies is a vital strategy for early cancer detection. Water-dispersable Fe/Fe3O4-core/shell based nanoplatforms for protease detection are capable of detecting protease activity down to sub-femtomolar limits of detection. They feature one dye (tetrakis(carboxyphenyl)porphyrin (TCPP)) that is tethered to the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis.
topic biophotonics
breast cancer
iron/iron oxide nanoparticle
liquid biopsy
nanodiagnostics detection
nanomedicine
sub-femtomolar limit of detection
url https://doi.org/10.3762/bjnano.7.33
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