Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
Proteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue s...
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doaj-5dfb1aeb2f4f489e9b23ff2bd73239e22020-11-25T01:00:11ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862016-03-017136437310.3762/bjnano.7.332190-4286-7-33Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detectionDinusha N. Udukala0Hongwang Wang1Sebastian O. Wendel2Aruni P. Malalasekera3Thilani N. Samarakoon4Asanka S. Yapa5Gayani Abayaweera6Matthew T. Basel7Pamela Maynez8Raquel Ortega9Yubisela Toledo10Leonie Bossmann11Colette Robinson12Katharine E. Janik13Olga B. Koper14Ping Li15Massoud Motamedi16Daniel A. Higgins17Gary Gadbury18Gaohong Zhu19Deryl L. Troyer20Stefan H. Bossmann21Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Anatomy & Physiology, 228 Coles Hall, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAThe University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAKansas State University, Department of Statistics, 101 Dickens Hall, Manhattan, KS, USAThe First Affiliated Hospital of Kunming Medical University, Department of Nuclear Medicine, 295 Xichang Road, Kunming, Yunnan, PR ChinaKansas State University, Department of Anatomy & Physiology, 228 Coles Hall, Manhattan, KS, USAKansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USAProteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue samples and liquid biopsies is a vital strategy for early cancer detection. Water-dispersable Fe/Fe3O4-core/shell based nanoplatforms for protease detection are capable of detecting protease activity down to sub-femtomolar limits of detection. They feature one dye (tetrakis(carboxyphenyl)porphyrin (TCPP)) that is tethered to the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis.https://doi.org/10.3762/bjnano.7.33biophotonicsbreast canceriron/iron oxide nanoparticleliquid biopsynanodiagnostics detectionnanomedicinesub-femtomolar limit of detection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dinusha N. Udukala Hongwang Wang Sebastian O. Wendel Aruni P. Malalasekera Thilani N. Samarakoon Asanka S. Yapa Gayani Abayaweera Matthew T. Basel Pamela Maynez Raquel Ortega Yubisela Toledo Leonie Bossmann Colette Robinson Katharine E. Janik Olga B. Koper Ping Li Massoud Motamedi Daniel A. Higgins Gary Gadbury Gaohong Zhu Deryl L. Troyer Stefan H. Bossmann |
spellingShingle |
Dinusha N. Udukala Hongwang Wang Sebastian O. Wendel Aruni P. Malalasekera Thilani N. Samarakoon Asanka S. Yapa Gayani Abayaweera Matthew T. Basel Pamela Maynez Raquel Ortega Yubisela Toledo Leonie Bossmann Colette Robinson Katharine E. Janik Olga B. Koper Ping Li Massoud Motamedi Daniel A. Higgins Gary Gadbury Gaohong Zhu Deryl L. Troyer Stefan H. Bossmann Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection Beilstein Journal of Nanotechnology biophotonics breast cancer iron/iron oxide nanoparticle liquid biopsy nanodiagnostics detection nanomedicine sub-femtomolar limit of detection |
author_facet |
Dinusha N. Udukala Hongwang Wang Sebastian O. Wendel Aruni P. Malalasekera Thilani N. Samarakoon Asanka S. Yapa Gayani Abayaweera Matthew T. Basel Pamela Maynez Raquel Ortega Yubisela Toledo Leonie Bossmann Colette Robinson Katharine E. Janik Olga B. Koper Ping Li Massoud Motamedi Daniel A. Higgins Gary Gadbury Gaohong Zhu Deryl L. Troyer Stefan H. Bossmann |
author_sort |
Dinusha N. Udukala |
title |
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection |
title_short |
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection |
title_full |
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection |
title_fullStr |
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection |
title_full_unstemmed |
Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection |
title_sort |
early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection |
publisher |
Beilstein-Institut |
series |
Beilstein Journal of Nanotechnology |
issn |
2190-4286 |
publishDate |
2016-03-01 |
description |
Proteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue samples and liquid biopsies is a vital strategy for early cancer detection. Water-dispersable Fe/Fe3O4-core/shell based nanoplatforms for protease detection are capable of detecting protease activity down to sub-femtomolar limits of detection. They feature one dye (tetrakis(carboxyphenyl)porphyrin (TCPP)) that is tethered to the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis. |
topic |
biophotonics breast cancer iron/iron oxide nanoparticle liquid biopsy nanodiagnostics detection nanomedicine sub-femtomolar limit of detection |
url |
https://doi.org/10.3762/bjnano.7.33 |
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