Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil
Abstract: INTRODUCTION Natural and artificial ecotope infestation by the kissing bug triatomines and their colonization and infection by Trypanosoma cruzi , the Chagas disease agent, were evaluated in nine municipalities of the State of Rio Grande do Norte, Brazil. METHODS Following identification...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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doaj-5e12590379b347769a1661e36dc4eb822020-11-24T22:46:47ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492016-02-01491576710.1590/0037-8682-0300-2015S0037-86822016000100057Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, BrazilAndressa Noronha Barbosa-SilvaAntonia Cláudia Jácome da CâmaraKiev MartinsDaniela Ferreira NunesPedro Igor Câmara de OliveiraPaulo Roberto Medeiros de AzevedoEgler ChiariLúcia Maria da Cunha GalvãoAbstract: INTRODUCTION Natural and artificial ecotope infestation by the kissing bug triatomines and their colonization and infection by Trypanosoma cruzi , the Chagas disease agent, were evaluated in nine municipalities of the State of Rio Grande do Norte, Brazil. METHODS Following identification, triatomine intestinal contents were analyzed by direct microscopic examination, xenoculture, and polymerase chain reaction (PCR) for parasite detection. Trypanosoma cruzi isolates were genotyped using three different markers. RESULTS Of 842 triatomines captured, 65% were Triatoma brasiliensis , 17.8% Triatoma pseudomaculata , 12.5% Panstrongylus lutzi , and 4.7% Rhodnius nasutus . Triatoma brasiliensis and P. lutzi adults were found in the intradomicile. T. brasiliensis, T. pseudomaculata , and R. nasutus nymphs and adults were found in the peridomicile and wild environment. Intradomiciliary and peridomiciliary infestation indexes were 5.6% and 33.7%, respectively. In the peridomicile, chicken coops were the most infested ecotope. The T. cruzi triatomine infection rate was 30.2%, of which PCR detected 29%. P . lutzi (78.1%), T . brasiliensis (24.5%), and T . pseudomaculata (22.7%) were the most infected species. TcII and III genotypes were detected in T. brasiliensis and TcIII in P. lutzi . CONCLUSIONS T. brasiliensis was found in all environments and most ecotopes with high T. cruzi infection rates. High infection rates were also detected in T . pseudomaculata and P. lutzi , suggesting their role in the interchange between the wild and peridomestic transmission cycles. The combination of PCR, microscopic examination, and xenoculture contributed to improving T. cruzi infection evaluation in triatomine bugs. The TcII and TcIII genotypes were predominant in the study area.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000100057&lng=en&tlng=enTrypanosoma cruziTriatominae natural infectionPCRXenoculture. Direct microscopic examination. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andressa Noronha Barbosa-Silva Antonia Cláudia Jácome da Câmara Kiev Martins Daniela Ferreira Nunes Pedro Igor Câmara de Oliveira Paulo Roberto Medeiros de Azevedo Egler Chiari Lúcia Maria da Cunha Galvão |
spellingShingle |
Andressa Noronha Barbosa-Silva Antonia Cláudia Jácome da Câmara Kiev Martins Daniela Ferreira Nunes Pedro Igor Câmara de Oliveira Paulo Roberto Medeiros de Azevedo Egler Chiari Lúcia Maria da Cunha Galvão Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil Revista da Sociedade Brasileira de Medicina Tropical Trypanosoma cruzi Triatominae natural infection PCR Xenoculture. Direct microscopic examination. |
author_facet |
Andressa Noronha Barbosa-Silva Antonia Cláudia Jácome da Câmara Kiev Martins Daniela Ferreira Nunes Pedro Igor Câmara de Oliveira Paulo Roberto Medeiros de Azevedo Egler Chiari Lúcia Maria da Cunha Galvão |
author_sort |
Andressa Noronha Barbosa-Silva |
title |
Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil |
title_short |
Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil |
title_full |
Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil |
title_fullStr |
Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil |
title_full_unstemmed |
Characteristics of Triatomine infestation and natural Trypanosoma cruzi infection in the State of Rio Grande do Norte, Brazil |
title_sort |
characteristics of triatomine infestation and natural trypanosoma cruzi infection in the state of rio grande do norte, brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
series |
Revista da Sociedade Brasileira de Medicina Tropical |
issn |
1678-9849 |
publishDate |
2016-02-01 |
description |
Abstract: INTRODUCTION Natural and artificial ecotope infestation by the kissing bug triatomines and their colonization and infection by Trypanosoma cruzi , the Chagas disease agent, were evaluated in nine municipalities of the State of Rio Grande do Norte, Brazil. METHODS Following identification, triatomine intestinal contents were analyzed by direct microscopic examination, xenoculture, and polymerase chain reaction (PCR) for parasite detection. Trypanosoma cruzi isolates were genotyped using three different markers. RESULTS Of 842 triatomines captured, 65% were Triatoma brasiliensis , 17.8% Triatoma pseudomaculata , 12.5% Panstrongylus lutzi , and 4.7% Rhodnius nasutus . Triatoma brasiliensis and P. lutzi adults were found in the intradomicile. T. brasiliensis, T. pseudomaculata , and R. nasutus nymphs and adults were found in the peridomicile and wild environment. Intradomiciliary and peridomiciliary infestation indexes were 5.6% and 33.7%, respectively. In the peridomicile, chicken coops were the most infested ecotope. The T. cruzi triatomine infection rate was 30.2%, of which PCR detected 29%. P . lutzi (78.1%), T . brasiliensis (24.5%), and T . pseudomaculata (22.7%) were the most infected species. TcII and III genotypes were detected in T. brasiliensis and TcIII in P. lutzi . CONCLUSIONS T. brasiliensis was found in all environments and most ecotopes with high T. cruzi infection rates. High infection rates were also detected in T . pseudomaculata and P. lutzi , suggesting their role in the interchange between the wild and peridomestic transmission cycles. The combination of PCR, microscopic examination, and xenoculture contributed to improving T. cruzi infection evaluation in triatomine bugs. The TcII and TcIII genotypes were predominant in the study area. |
topic |
Trypanosoma cruzi Triatominae natural infection PCR Xenoculture. Direct microscopic examination. |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000100057&lng=en&tlng=en |
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