Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection
Gold nanoparticles (AuNPs) have been used to increase the power of electrochemical biosensors. However, densities and pattern distributions of AuNPs colloids have not been determined yet. In this report, we attempt identifying coverable densities of AuNPs on interdigitated electrodes (IDEs) and incr...
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doaj-5e1a8d3d4bda49cb8b04b51599a929d62020-11-25T01:23:36ZengElsevierSensing and Bio-Sensing Research2214-18042020-02-0127Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detectionThanh T. Vu0Sojin Song1Hien D.N. Lai2Ngoc Lan Mai3Thuat T. Trinh4Ha T. Do5Dai Phu- Huynh6Anh H. Nguyen7Faculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu Tho Str., Tan Phong Ward, District 7, Ho Chi Minh City, Viet NamDepartment of Chemical and Biological Engineering, Korea University, Seoul 136-713, South KoreaElectronic Engineering Department, Kyunghee University, Yongin 17104, South KoreaFaculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu Tho Str., Tan Phong Ward, District 7, Ho Chi Minh City, Viet NamFaculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu Tho Str., Tan Phong Ward, District 7, Ho Chi Minh City, Viet Nam; Department of Civil- and Environmental Engineering, Norwegian University of Science and Technology, 7491 Trondheim, NorwayFaculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu Tho Str., Tan Phong Ward, District 7, Ho Chi Minh City, Viet NamFaculty of Materials Technology, Ho Chi Minh City University of Technology, VNU – HCM, Viet Nam.; Polymer research Center, Ho Chi Minh City University of Technology, VNU – HCM, Viet Nam.Faculty of Applied Sciences, Ton Duc Thang University, 19 Nguyen Huu Tho Str., Tan Phong Ward, District 7, Ho Chi Minh City, Viet Nam; Corresponding author.Gold nanoparticles (AuNPs) have been used to increase the power of electrochemical biosensors. However, densities and pattern distributions of AuNPs colloids have not been determined yet. In this report, we attempt identifying coverable densities of AuNPs on interdigitated electrodes (IDEs) and increasing the sensing signal by using cyclic antigen (CCP)-coated-AuNPs in impedance biosensors. IDEs were covalently functionalized with CCP-coated AuNPs and used for detection of anti-CCP antibody (ACPA), a remarkable biomarker for rheumatoid arthritis. 36% and 82% of ACPA in human commercial serum were captured by a low CCP (300 CCP per 40-nm nanoparticles) and high CCP (17 × 105 CCP per 40-nm nanoparticles) densities of colloids, respectively. Coverage of the colloids on gaps between two digits of IDEs were observed about at 2.7 × 106 AuNPs (30 nm) per 1 μm2 for low signal-to-noise ratio (SNR). The enzyme-catalyzed deposition of silver nanocrystal increased the SNR of the impedance signal up to 7.8 folds of magnitude higher than un-deposited counterparts. Sensitivity and limit of detection were determined at 1.3 ng/mL and 0.12 ng/mL, respectively, at a frequency 900 kHz in the range of 200–2000 kHz. Finally, Principal Component Analysis (PCA) showed the ability of the biosensor to follow variation over time of the number of CCP and therefore also the quantify of ACPA in the samples. Keywords: Impedance biosensor, Rheumatoid arthritis, Cyclic citrullinated peptide, Label-free detectionhttp://www.sciencedirect.com/science/article/pii/S2214180419301540 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thanh T. Vu Sojin Song Hien D.N. Lai Ngoc Lan Mai Thuat T. Trinh Ha T. Do Dai Phu- Huynh Anh H. Nguyen |
spellingShingle |
Thanh T. Vu Sojin Song Hien D.N. Lai Ngoc Lan Mai Thuat T. Trinh Ha T. Do Dai Phu- Huynh Anh H. Nguyen Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection Sensing and Bio-Sensing Research |
author_facet |
Thanh T. Vu Sojin Song Hien D.N. Lai Ngoc Lan Mai Thuat T. Trinh Ha T. Do Dai Phu- Huynh Anh H. Nguyen |
author_sort |
Thanh T. Vu |
title |
Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection |
title_short |
Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection |
title_full |
Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection |
title_fullStr |
Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection |
title_full_unstemmed |
Coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection |
title_sort |
coverage degrees of colloids on electrochemical electrodes and signal amplification for anti-citrullinated peptide antibody detection |
publisher |
Elsevier |
series |
Sensing and Bio-Sensing Research |
issn |
2214-1804 |
publishDate |
2020-02-01 |
description |
Gold nanoparticles (AuNPs) have been used to increase the power of electrochemical biosensors. However, densities and pattern distributions of AuNPs colloids have not been determined yet. In this report, we attempt identifying coverable densities of AuNPs on interdigitated electrodes (IDEs) and increasing the sensing signal by using cyclic antigen (CCP)-coated-AuNPs in impedance biosensors. IDEs were covalently functionalized with CCP-coated AuNPs and used for detection of anti-CCP antibody (ACPA), a remarkable biomarker for rheumatoid arthritis. 36% and 82% of ACPA in human commercial serum were captured by a low CCP (300 CCP per 40-nm nanoparticles) and high CCP (17 × 105 CCP per 40-nm nanoparticles) densities of colloids, respectively. Coverage of the colloids on gaps between two digits of IDEs were observed about at 2.7 × 106 AuNPs (30 nm) per 1 μm2 for low signal-to-noise ratio (SNR). The enzyme-catalyzed deposition of silver nanocrystal increased the SNR of the impedance signal up to 7.8 folds of magnitude higher than un-deposited counterparts. Sensitivity and limit of detection were determined at 1.3 ng/mL and 0.12 ng/mL, respectively, at a frequency 900 kHz in the range of 200–2000 kHz. Finally, Principal Component Analysis (PCA) showed the ability of the biosensor to follow variation over time of the number of CCP and therefore also the quantify of ACPA in the samples. Keywords: Impedance biosensor, Rheumatoid arthritis, Cyclic citrullinated peptide, Label-free detection |
url |
http://www.sciencedirect.com/science/article/pii/S2214180419301540 |
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