Retinoic acid inhibition impairs planarian eye regeneration.
Retinoic acid is a known morphogen in regulating animal growth and development. Planaria are a key model system for regeneration and their eyes are a morphological marker of anterior differentiation. We explored the requirement for retinoic acid signaling in the regeneration of body parts in the pla...
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Appalachian State University Honors College
2019-10-01
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Online Access: | https://impulse.appstate.edu/articles/2019/retinoic-acid-inhibition-impairs-planarian-eye-regeneration |
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doaj-5e26f4deff3242f087f1a8c855fe13b62020-11-25T01:51:49ZengAppalachian State University Honors CollegeImpulse: The Premier Undergraduate Neuroscience Journal1934-33611934-33612019-10-01Retinoic acid inhibition impairs planarian eye regeneration.Gabrielle Bennetti0Aaron Corbin-Leftwich1Linda M. Boland2Isaac Skromne3Department of Biology and Neuroscience Program, University of Richmond, Richmond, VA 23173Department of Biology and Neuroscience Program, University of Richmond, Richmond, VA 23173Department of Biology and Neuroscience Program, University of Richmond, Richmond, VA 23173Department of Biology and Neuroscience Program, University of Richmond, Richmond, VA 23173Retinoic acid is a known morphogen in regulating animal growth and development. Planaria are a key model system for regeneration and their eyes are a morphological marker of anterior differentiation. We explored the requirement for retinoic acid signaling in the regeneration of body parts in the planaria S. mediterranea using an inhibitor of retinoic acid synthesis, diethylaminobenzaldehyde (DEAB). Whole planaria, soaked in DEAB for three days prior to and five days following amputation, produced trunk and tail fragments with defective anterior regeneration. Following regeneration, up to 80% of posterior fragments developed abnormal eyes. The abnormalities included animals without eyes, with only a single eye, with one enlarged eye, or two eyes of different sizes. Eyes were considered to be functional because animals responded to blue laser light with turning behavior. No abnormalities in eye regeneration were observed in side by side vehicle controls. These results suggest that retinoic acid is necessary for normal eye regeneration following injury and supports a previously undocumented signaling role in planaria eye development. https://impulse.appstate.edu/articles/2019/retinoic-acid-inhibition-impairs-planarian-eye-regenerationAmputationEyespotRegenerationPlanariaStem CellsRetinoic AcidMorphogen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabrielle Bennetti Aaron Corbin-Leftwich Linda M. Boland Isaac Skromne |
spellingShingle |
Gabrielle Bennetti Aaron Corbin-Leftwich Linda M. Boland Isaac Skromne Retinoic acid inhibition impairs planarian eye regeneration. Impulse: The Premier Undergraduate Neuroscience Journal Amputation Eyespot Regeneration Planaria Stem Cells Retinoic Acid Morphogen |
author_facet |
Gabrielle Bennetti Aaron Corbin-Leftwich Linda M. Boland Isaac Skromne |
author_sort |
Gabrielle Bennetti |
title |
Retinoic acid inhibition impairs planarian eye regeneration. |
title_short |
Retinoic acid inhibition impairs planarian eye regeneration. |
title_full |
Retinoic acid inhibition impairs planarian eye regeneration. |
title_fullStr |
Retinoic acid inhibition impairs planarian eye regeneration. |
title_full_unstemmed |
Retinoic acid inhibition impairs planarian eye regeneration. |
title_sort |
retinoic acid inhibition impairs planarian eye regeneration. |
publisher |
Appalachian State University Honors College |
series |
Impulse: The Premier Undergraduate Neuroscience Journal |
issn |
1934-3361 1934-3361 |
publishDate |
2019-10-01 |
description |
Retinoic acid is a known morphogen in regulating animal growth and development. Planaria are a key model system for regeneration and their eyes are a morphological marker of anterior differentiation. We explored the requirement for retinoic acid signaling in the regeneration of body parts in the planaria S. mediterranea using an inhibitor of retinoic acid synthesis, diethylaminobenzaldehyde (DEAB). Whole planaria, soaked in DEAB for three days prior to and five days following amputation, produced trunk and tail fragments with defective anterior regeneration. Following regeneration, up to 80% of posterior fragments developed abnormal eyes. The abnormalities included animals without eyes, with only a single eye, with one enlarged eye, or two eyes of different sizes. Eyes were considered to be functional because animals responded to blue laser light with turning behavior. No abnormalities in eye regeneration were observed in side by side vehicle controls. These results suggest that retinoic acid is necessary for normal eye regeneration following injury and supports a previously undocumented signaling role in planaria eye development.
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topic |
Amputation Eyespot Regeneration Planaria Stem Cells Retinoic Acid Morphogen |
url |
https://impulse.appstate.edu/articles/2019/retinoic-acid-inhibition-impairs-planarian-eye-regeneration |
work_keys_str_mv |
AT gabriellebennetti retinoicacidinhibitionimpairsplanarianeyeregeneration AT aaroncorbinleftwich retinoicacidinhibitionimpairsplanarianeyeregeneration AT lindamboland retinoicacidinhibitionimpairsplanarianeyeregeneration AT isaacskromne retinoicacidinhibitionimpairsplanarianeyeregeneration |
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