OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis
Ovate Family Protein1 (OFP1) is a regulator, and it is suspected to be involved in plant growth and development. Meanwhile, Arabidopsis Thaliana Homeobox (ATH1), a BEL1-like homeodomain (HD) transcription factor, is known to be involved in regulating stem growth, flowering time and flower basal boun...
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doaj-5e2a8a4d8cf54d52b3490227c61ced282020-11-24T23:13:09ZengMDPI AGGenes2073-44252018-08-019839910.3390/genes9080399genes9080399OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in ArabidopsisLiguo Zhang0Lili Sun1Xiaofei Zhang2Shuquan Zhang3Dongwei Xie4Chunbo Liang5Wengong Huang6Lijuan Fan7Yuyan Fang8Ying Chang9Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaCollege of Mathematics and Information Sciences, Guangxi University, Nanning 530004, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaCollege of Life Science, Northeast Agricultural University, Harbin 150030, ChinaOvate Family Protein1 (OFP1) is a regulator, and it is suspected to be involved in plant growth and development. Meanwhile, Arabidopsis Thaliana Homeobox (ATH1), a BEL1-like homeodomain (HD) transcription factor, is known to be involved in regulating stem growth, flowering time and flower basal boundary development in Arabidopsis. Previous large-scale yeast two-hybrid studies suggest that ATH1 possibly interact with OFP1, but this interaction is yet unverified. In our study, the interaction of OFP1 with ATH1 was verified using a directional yeast two-hybrid system and bimolecular fluorescence complementation (BiFC). Our results also demonstrated that the OFP1-ATH1 interaction is mainly controlled by the HD domain of ATH1. Meanwhile, we found that ATH1 plays the role of transcriptional repressor to regulate plant development and that OFP1 can enhance ATH1 repression function. Regardless of the mechanism, a putative functional role of ATH1-OFP1 may be to regulate the expression of the both the GA20ox1 gene, which is involved in gibberellin (GA) biosynthesis and control of stem elongation, and the Flowering Locus C (FLC) gene, which inhibits transition to flowering. Ultimately, the regulatory functional mechanism of OFP1-ATH1 may be complicated and diverse according to our results, and this work lays groundwork for further understanding of a unique and important protein–protein interaction that influences flowering time, stem development, and flower basal boundary development in plants.http://www.mdpi.com/2073-4425/9/8/399OFP1ATH1interactionstem elongationfloweringboundary |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liguo Zhang Lili Sun Xiaofei Zhang Shuquan Zhang Dongwei Xie Chunbo Liang Wengong Huang Lijuan Fan Yuyan Fang Ying Chang |
spellingShingle |
Liguo Zhang Lili Sun Xiaofei Zhang Shuquan Zhang Dongwei Xie Chunbo Liang Wengong Huang Lijuan Fan Yuyan Fang Ying Chang OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis Genes OFP1 ATH1 interaction stem elongation flowering boundary |
author_facet |
Liguo Zhang Lili Sun Xiaofei Zhang Shuquan Zhang Dongwei Xie Chunbo Liang Wengong Huang Lijuan Fan Yuyan Fang Ying Chang |
author_sort |
Liguo Zhang |
title |
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis |
title_short |
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis |
title_full |
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis |
title_fullStr |
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis |
title_full_unstemmed |
OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis |
title_sort |
ofp1 interaction with ath1 regulates stem growth, flowering time and flower basal boundary formation in arabidopsis |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2018-08-01 |
description |
Ovate Family Protein1 (OFP1) is a regulator, and it is suspected to be involved in plant growth and development. Meanwhile, Arabidopsis Thaliana Homeobox (ATH1), a BEL1-like homeodomain (HD) transcription factor, is known to be involved in regulating stem growth, flowering time and flower basal boundary development in Arabidopsis. Previous large-scale yeast two-hybrid studies suggest that ATH1 possibly interact with OFP1, but this interaction is yet unverified. In our study, the interaction of OFP1 with ATH1 was verified using a directional yeast two-hybrid system and bimolecular fluorescence complementation (BiFC). Our results also demonstrated that the OFP1-ATH1 interaction is mainly controlled by the HD domain of ATH1. Meanwhile, we found that ATH1 plays the role of transcriptional repressor to regulate plant development and that OFP1 can enhance ATH1 repression function. Regardless of the mechanism, a putative functional role of ATH1-OFP1 may be to regulate the expression of the both the GA20ox1 gene, which is involved in gibberellin (GA) biosynthesis and control of stem elongation, and the Flowering Locus C (FLC) gene, which inhibits transition to flowering. Ultimately, the regulatory functional mechanism of OFP1-ATH1 may be complicated and diverse according to our results, and this work lays groundwork for further understanding of a unique and important protein–protein interaction that influences flowering time, stem development, and flower basal boundary development in plants. |
topic |
OFP1 ATH1 interaction stem elongation flowering boundary |
url |
http://www.mdpi.com/2073-4425/9/8/399 |
work_keys_str_mv |
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