Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p
Background/Aims: This study focused on the oncogenic role of Diphthamide biosynthesis 1 (DPH1) in colorectal cancer (CRC) cells. Methods: The expression of DPH1 was determined by quantitative RT-PCR analysis and western blotting in CRC tissues. The role of DPH1 in CRC cells was investigated via cell...
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Cell Physiol Biochem Press GmbH & Co KG
2017-11-01
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doaj-5e2baa1ba0cd49f1a1d4f3d96548fd402020-11-24T22:09:34ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-0144250551410.1159/000485087485087Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5pMinghui LiuKai YinXu GuoHuijin FengMin YuanYanqing LiuJianguo ZhangBaoliang GuoChen WangGuangxin ZhouZhen ZhouChen-Yu ZhangXi ChenBackground/Aims: This study focused on the oncogenic role of Diphthamide biosynthesis 1 (DPH1) in colorectal cancer (CRC) cells. Methods: The expression of DPH1 was determined by quantitative RT-PCR analysis and western blotting in CRC tissues. The role of DPH1 in CRC cells was investigated via cell viability and invasion assays under the condition of DPH1 silencing or overexpression. Bioinformatics analysis and luciferase reporter analysis were used to identify the upstream microRNA which might regulate DPH1.The inverse correlation between the microRNA and DPH1 was also detected in CRC cells. Results: We identified an unexpected role for DPH1 as an oncogene in CRC cells. The tumour-suppressive miR-218-5p regulates DPH1 directly and negatively. Loss of miR-218-5p drives the oncogenic role of DPH1 in CRC cells. Conclusion: The modulation of DPH1 by miR-218-5p may be an important regulatory axis during CRCtumourigenesis.https://www.karger.com/Article/FullText/485087TumourigenesisCRCDph1MiR-218-5p |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Minghui Liu Kai Yin Xu Guo Huijin Feng Min Yuan Yanqing Liu Jianguo Zhang Baoliang Guo Chen Wang Guangxin Zhou Zhen Zhou Chen-Yu Zhang Xi Chen |
spellingShingle |
Minghui Liu Kai Yin Xu Guo Huijin Feng Min Yuan Yanqing Liu Jianguo Zhang Baoliang Guo Chen Wang Guangxin Zhou Zhen Zhou Chen-Yu Zhang Xi Chen Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p Cellular Physiology and Biochemistry Tumourigenesis CRC Dph1 MiR-218-5p |
author_facet |
Minghui Liu Kai Yin Xu Guo Huijin Feng Min Yuan Yanqing Liu Jianguo Zhang Baoliang Guo Chen Wang Guangxin Zhou Zhen Zhou Chen-Yu Zhang Xi Chen |
author_sort |
Minghui Liu |
title |
Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p |
title_short |
Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p |
title_full |
Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p |
title_fullStr |
Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p |
title_full_unstemmed |
Diphthamide Biosynthesis 1 is a Novel Oncogene in Colorectal Cancer Cells and is Regulated by MiR-218-5p |
title_sort |
diphthamide biosynthesis 1 is a novel oncogene in colorectal cancer cells and is regulated by mir-218-5p |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2017-11-01 |
description |
Background/Aims: This study focused on the oncogenic role of Diphthamide biosynthesis 1 (DPH1) in colorectal cancer (CRC) cells. Methods: The expression of DPH1 was determined by quantitative RT-PCR analysis and western blotting in CRC tissues. The role of DPH1 in CRC cells was investigated via cell viability and invasion assays under the condition of DPH1 silencing or overexpression. Bioinformatics analysis and luciferase reporter analysis were used to identify the upstream microRNA which might regulate DPH1.The inverse correlation between the microRNA and DPH1 was also detected in CRC cells. Results: We identified an unexpected role for DPH1 as an oncogene in CRC cells. The tumour-suppressive miR-218-5p regulates DPH1 directly and negatively. Loss of miR-218-5p drives the oncogenic role of DPH1 in CRC cells. Conclusion: The modulation of DPH1 by miR-218-5p may be an important regulatory axis during CRCtumourigenesis. |
topic |
Tumourigenesis CRC Dph1 MiR-218-5p |
url |
https://www.karger.com/Article/FullText/485087 |
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