Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms
The purpose of this research was to characterize the antibiotic resistance patterns of <i>Campylobacter</i> spp. isolated from commercial farrow to finish farms in Greece, and analyze the relevant molecular resistance mechanisms among the resistant <i>Campylobacter</i> isolat...
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doaj-5e383db970a047778fe5f1e7f9d692172021-08-26T13:27:26ZengMDPI AGAnimals2076-26152021-08-01112394239410.3390/ani11082394Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig FarmsDimitrios Papadopoulos0Evanthia Petridou1Konstantinos Papageorgiou2Ioannis A. Giantsis3Georgios Delis4Vangelis Economou5Ilias Frydas6Georgios Papadopoulos7Maria Hatzistylianou8Spyridon K. Kritas9Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceFaculty of Agricultural Sciences, University of Western Macedonia, 53100 Florina, GreeceLaboratory of Pharmacology, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Hygiene of Food of Animal Origin-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Science, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceClinic of Pediatrics-Immunology and Infectious Diseases, Faculty of Health Sciences, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceThe purpose of this research was to characterize the antibiotic resistance patterns of <i>Campylobacter</i> spp. isolated from commercial farrow to finish farms in Greece, and analyze the relevant molecular resistance mechanisms among the resistant <i>Campylobacter</i> isolates. Susceptibility testing to five different classes of antibiotics was performed in 100 <i>C. coli</i> and 100 <i>C. jejuni</i>, previously isolated and identified. All isolates were found susceptible to meropenem. Very high rates of resistance were recorded for tetracyclines (84.5%), medium rates of resistance were recorded regarding quinolones (23%), and low and very low rates of resistance were identified for macrolides such as erythromycin and aminoglycosides (12% and 4%, respectively). Only 12.5% of the <i>Campylobacter</i> isolates displayed MDR. Regarding the molecular mechanisms of resistance, all ciprofloxacin resistant isolates hosted the mutant type <i>Thr-86-Ile</i> region of the quinolone resistance-determining region (QRDR) of the <i>gyrA</i> gene. In all erythromycin resistant isolates, the transitional mutations A2075G and A2074C in the <i>23S rRNA</i> gene were only amplified. Molecular screening of tetracycline resistance genes indicated that the vast majority of <i>Campylobacter</i> isolates (92.3%) were positive for the <i>tet(O)</i> gene. In summary, these findings and especially the very high and medium rates of resistance for tetracyclines and fluroquinolones, respectively recommend that a continuous monitoring of <i>Campylobacter</i> isolates susceptibility in combination with the proper use of antimicrobials in livestock production is of great importance for public health.https://www.mdpi.com/2076-2615/11/8/2394<i>Campylobacter</i>pigsantimicrobial resistance |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dimitrios Papadopoulos Evanthia Petridou Konstantinos Papageorgiou Ioannis A. Giantsis Georgios Delis Vangelis Economou Ilias Frydas Georgios Papadopoulos Maria Hatzistylianou Spyridon K. Kritas |
spellingShingle |
Dimitrios Papadopoulos Evanthia Petridou Konstantinos Papageorgiou Ioannis A. Giantsis Georgios Delis Vangelis Economou Ilias Frydas Georgios Papadopoulos Maria Hatzistylianou Spyridon K. Kritas Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms Animals <i>Campylobacter</i> pigs antimicrobial resistance |
author_facet |
Dimitrios Papadopoulos Evanthia Petridou Konstantinos Papageorgiou Ioannis A. Giantsis Georgios Delis Vangelis Economou Ilias Frydas Georgios Papadopoulos Maria Hatzistylianou Spyridon K. Kritas |
author_sort |
Dimitrios Papadopoulos |
title |
Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms |
title_short |
Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms |
title_full |
Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms |
title_fullStr |
Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms |
title_full_unstemmed |
Phenotypic and Molecular Patterns of Resistance among <i>Campylobacter coli</i> and <i>Campylobacter jejuni</i> Isolates, from Pig Farms |
title_sort |
phenotypic and molecular patterns of resistance among <i>campylobacter coli</i> and <i>campylobacter jejuni</i> isolates, from pig farms |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-08-01 |
description |
The purpose of this research was to characterize the antibiotic resistance patterns of <i>Campylobacter</i> spp. isolated from commercial farrow to finish farms in Greece, and analyze the relevant molecular resistance mechanisms among the resistant <i>Campylobacter</i> isolates. Susceptibility testing to five different classes of antibiotics was performed in 100 <i>C. coli</i> and 100 <i>C. jejuni</i>, previously isolated and identified. All isolates were found susceptible to meropenem. Very high rates of resistance were recorded for tetracyclines (84.5%), medium rates of resistance were recorded regarding quinolones (23%), and low and very low rates of resistance were identified for macrolides such as erythromycin and aminoglycosides (12% and 4%, respectively). Only 12.5% of the <i>Campylobacter</i> isolates displayed MDR. Regarding the molecular mechanisms of resistance, all ciprofloxacin resistant isolates hosted the mutant type <i>Thr-86-Ile</i> region of the quinolone resistance-determining region (QRDR) of the <i>gyrA</i> gene. In all erythromycin resistant isolates, the transitional mutations A2075G and A2074C in the <i>23S rRNA</i> gene were only amplified. Molecular screening of tetracycline resistance genes indicated that the vast majority of <i>Campylobacter</i> isolates (92.3%) were positive for the <i>tet(O)</i> gene. In summary, these findings and especially the very high and medium rates of resistance for tetracyclines and fluroquinolones, respectively recommend that a continuous monitoring of <i>Campylobacter</i> isolates susceptibility in combination with the proper use of antimicrobials in livestock production is of great importance for public health. |
topic |
<i>Campylobacter</i> pigs antimicrobial resistance |
url |
https://www.mdpi.com/2076-2615/11/8/2394 |
work_keys_str_mv |
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