HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer.
<h4>Objective</h4>The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).<h4>Methods</h4>Firstly, HHIP gene expression data of NSCL...
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doaj-5e3f3e0c29f4493ea3a24f2ad70dfda02021-03-04T10:22:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011411e022575510.1371/journal.pone.0225755HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer.Jian-Guo ZhaoJian-Fang WangJiang-Feng FengXue-Ying JinWan-Li Ye<h4>Objective</h4>The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).<h4>Methods</h4>Firstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP.<h4>Results</h4>HHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (P<0.05). This result was confirmed in NSCLC cell lines by qRT-PCR analysis, its expression in normal NSCLC cell line BEAS-2B was significantly higher than that in NSCLC cells. Chi-square test results showed that the low expression of HHIP was correlated with gender, cancer type, TNM stage and tumor size. Functional experimental results showed that over-expressing HHIP significantly decreased the ability of cell proliferation, migration and invasion in NSCLC cells (P<0.05).<h4>Conclusion</h4>Overall, the above results indicated that HHIP could regulate proliferation, migration and invasion, and could be used as a judging criterion for identifying NSCLC classification and stage.https://doi.org/10.1371/journal.pone.0225755 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jian-Guo Zhao Jian-Fang Wang Jiang-Feng Feng Xue-Ying Jin Wan-Li Ye |
spellingShingle |
Jian-Guo Zhao Jian-Fang Wang Jiang-Feng Feng Xue-Ying Jin Wan-Li Ye HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. PLoS ONE |
author_facet |
Jian-Guo Zhao Jian-Fang Wang Jiang-Feng Feng Xue-Ying Jin Wan-Li Ye |
author_sort |
Jian-Guo Zhao |
title |
HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. |
title_short |
HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. |
title_full |
HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. |
title_fullStr |
HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. |
title_full_unstemmed |
HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. |
title_sort |
hhip overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
<h4>Objective</h4>The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).<h4>Methods</h4>Firstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP.<h4>Results</h4>HHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (P<0.05). This result was confirmed in NSCLC cell lines by qRT-PCR analysis, its expression in normal NSCLC cell line BEAS-2B was significantly higher than that in NSCLC cells. Chi-square test results showed that the low expression of HHIP was correlated with gender, cancer type, TNM stage and tumor size. Functional experimental results showed that over-expressing HHIP significantly decreased the ability of cell proliferation, migration and invasion in NSCLC cells (P<0.05).<h4>Conclusion</h4>Overall, the above results indicated that HHIP could regulate proliferation, migration and invasion, and could be used as a judging criterion for identifying NSCLC classification and stage. |
url |
https://doi.org/10.1371/journal.pone.0225755 |
work_keys_str_mv |
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1714806312783577088 |