Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires
Abstract Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosph...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-01-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-81789-1 |
id |
doaj-5e7f36d784974374a79e7aed0446c08d |
---|---|
record_format |
Article |
spelling |
doaj-5e7f36d784974374a79e7aed0446c08d2021-01-31T16:25:26ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111210.1038/s41598-021-81789-1Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfiresSanja Babić0Lara Čižmek1Aleksandra Maršavelski2Olga Malev3Maryline Pflieger4Ivančica Strunjak-Perović5Natalija Topić Popović6Rozelindra Čož-Rakovac7Polonca Trebše8Laboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruđer Bošković InstituteLaboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruđer Bošković InstituteFaculty of Science, Department of Chemistry, University of ZagrebFaculty of Science, Department of Biology, University of ZagrebFaculty of Health Sciences, University of LjubljanaLaboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruđer Bošković InstituteLaboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruđer Bošković InstituteLaboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruđer Bošković InstituteFaculty of Health Sciences, University of LjubljanaAbstract Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4,6-dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4,6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments.https://doi.org/10.1038/s41598-021-81789-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sanja Babić Lara Čižmek Aleksandra Maršavelski Olga Malev Maryline Pflieger Ivančica Strunjak-Perović Natalija Topić Popović Rozelindra Čož-Rakovac Polonca Trebše |
spellingShingle |
Sanja Babić Lara Čižmek Aleksandra Maršavelski Olga Malev Maryline Pflieger Ivančica Strunjak-Perović Natalija Topić Popović Rozelindra Čož-Rakovac Polonca Trebše Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires Scientific Reports |
author_facet |
Sanja Babić Lara Čižmek Aleksandra Maršavelski Olga Malev Maryline Pflieger Ivančica Strunjak-Perović Natalija Topić Popović Rozelindra Čož-Rakovac Polonca Trebše |
author_sort |
Sanja Babić |
title |
Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires |
title_short |
Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires |
title_full |
Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires |
title_fullStr |
Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires |
title_full_unstemmed |
Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires |
title_sort |
utilization of the zebrafish model to unravel the harmful effects of biomass burning during amazonian wildfires |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4,6-dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4,6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments. |
url |
https://doi.org/10.1038/s41598-021-81789-1 |
work_keys_str_mv |
AT sanjababic utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT laracizmek utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT aleksandramarsavelski utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT olgamalev utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT marylinepflieger utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT ivancicastrunjakperovic utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT natalijatopicpopovic utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT rozelindracozrakovac utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires AT poloncatrebse utilizationofthezebrafishmodeltounraveltheharmfuleffectsofbiomassburningduringamazonianwildfires |
_version_ |
1724316300722831360 |