Effect of chitosan, antagonist and endophytic bacteria in suppressing the development of leaf spot disease in kopyor coconut seedlings

• Main Authors: Deden Dewantara Eris, Sri wahyuni, Imron Riyadi, Happy Widiastuti, Siswanto
• Format: Article
• Language: English
• Published: indonesian research institute for biotechnology and bioindustry 2019-04-01
• Series: Menara Perkebunan
• Subjects: coconut kopyor seedling; leaf spot disease; antagonist microbes; endophytic bacteria; chitosan
• Online Access: http://mp.iribb.org/index.php/mpjurnal/article/view/324

Currently, tissue culture is one of the best techniques in propagating of kopyor coconut. The important stage in plant propagation through tissue culture is acclimatization. Upon entering the acclimatization stage, the problem that could arise in kopyor coconut seedlings is leaf spot disease caused by the fungus Colletotrichum sp., Curvularia sp. and Pestalotiopsis sp. Environmentally friendly leaf spot disease control techniques can be done through the use of chitosan, antagonists microbes and endophytic bacteria. This study aimed to examine the use of chitosan, microbial antagonists, endophytic bacteria and their combinations to control leaf spot kopyor coconut disease in four different disease severity categories, namely severe/heavy, moderate, mild, and healthy. Disease development was observed every three weeks, while the rate of disease infection, area under the disease development curve (AUDPC) and disease progression/delta were observed 15 weeks after treatment. The result showed that in heavy severity category, endophytic bacteria treatment was more effective to inhibit leaf spot disease showed by AUDPC value of 131.95 units that significantly different compared to others. In the moderate category, combination treatment was more effective to suppress leaf spot disease, showed by the lowest infection rate by 0.03 unit per week, and percentage disease value progression changes was 12.10%, with no significantly different AUDPC value to the other treatments. In mild and healthy severity category, there were no significanly different between the rate of infection and AUDPC in all treatments. While the percentage of change progression disease was significantly different between endophytic treatment and control.