Examination of the Anaerobic Growth of Campylobacter concisus Strains
Campylobacter concisus is an oral bacterium that is associated with intestinal diseases. C. concisus was previously described as a bacterium that requires H2-enriched microaerobic conditions for growth. The level of H2 in the oral cavity is extremely low, suggesting that C. concisus is unlikely to...
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doaj-5ebff21be1864817a0c33ae6f390c98b2021-07-02T03:05:02ZengHindawi LimitedInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/476047476047Examination of the Anaerobic Growth of Campylobacter concisus StrainsHoyul Lee0Rena Ma1Michael C. Grimm2Stephen M. Riordan3Ruiting Lan4Ling Zhong5Mark Raftery6Li Zhang7The School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, Sydney, NSW 2052, AustraliaThe School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, Sydney, NSW 2052, AustraliaSt George and Sutherland Clinical School, University of New South Wales, Sydney, NSW 2052, AustraliaGastrointestinal and Liver Unit, Prince of Wales Hospital and Prince of Wales Clinical School, University of New South Wales, Sydney, NSW 2052, AustraliaThe School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, Sydney, NSW 2052, AustraliaBioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, NSW 2052, AustraliaBioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, NSW 2052, AustraliaThe School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, Sydney, NSW 2052, AustraliaCampylobacter concisus is an oral bacterium that is associated with intestinal diseases. C. concisus was previously described as a bacterium that requires H2-enriched microaerobic conditions for growth. The level of H2 in the oral cavity is extremely low, suggesting that C. concisus is unlikely to have a microaerobic growth there. In this study, the anaerobic growth of C. concisus was investigated. The growth of fifty-seven oral C. concisus strains and six enteric C. concisus strains under various atmospheric conditions including anaerobic conditions with and without H2 was examined. The atmospheric conditions were generated using commercially available gas-generation systems. C. concisus putative virulence proteins were identified using mass spectrometry analysis. Under anaerobic conditions, 92% of the oral C. concisus strains (52/57) and all six enteric strains grew without the presence of H2 and the presence of H2 greatly increased C. concisus growth. An oral C. concisus strain was found to express a number of putative virulence proteins and the expression levels of these proteins were not affected by H2. The levels of H2 appeared to affect the optimal growth of C. concisus. This study provides useful information in understanding the natural colonization site and pathogenicity of C. concisus.http://dx.doi.org/10.1155/2014/476047 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hoyul Lee Rena Ma Michael C. Grimm Stephen M. Riordan Ruiting Lan Ling Zhong Mark Raftery Li Zhang |
spellingShingle |
Hoyul Lee Rena Ma Michael C. Grimm Stephen M. Riordan Ruiting Lan Ling Zhong Mark Raftery Li Zhang Examination of the Anaerobic Growth of Campylobacter concisus Strains International Journal of Microbiology |
author_facet |
Hoyul Lee Rena Ma Michael C. Grimm Stephen M. Riordan Ruiting Lan Ling Zhong Mark Raftery Li Zhang |
author_sort |
Hoyul Lee |
title |
Examination of the Anaerobic Growth of Campylobacter concisus Strains |
title_short |
Examination of the Anaerobic Growth of Campylobacter concisus Strains |
title_full |
Examination of the Anaerobic Growth of Campylobacter concisus Strains |
title_fullStr |
Examination of the Anaerobic Growth of Campylobacter concisus Strains |
title_full_unstemmed |
Examination of the Anaerobic Growth of Campylobacter concisus Strains |
title_sort |
examination of the anaerobic growth of campylobacter concisus strains |
publisher |
Hindawi Limited |
series |
International Journal of Microbiology |
issn |
1687-918X 1687-9198 |
publishDate |
2014-01-01 |
description |
Campylobacter concisus is an oral bacterium that is associated with intestinal diseases. C. concisus was previously described as a bacterium that requires H2-enriched microaerobic conditions for growth. The level of H2 in the oral cavity is extremely low, suggesting that C. concisus is unlikely to have a microaerobic growth there. In this study, the anaerobic growth of C. concisus was investigated. The growth of fifty-seven oral C. concisus strains and six enteric C. concisus strains under various atmospheric conditions including anaerobic conditions with and without H2 was examined. The atmospheric conditions were generated using commercially available gas-generation systems. C. concisus putative virulence proteins were identified using mass spectrometry analysis. Under anaerobic conditions, 92% of the oral C. concisus strains (52/57) and all six enteric strains grew without the presence of H2 and the presence of H2 greatly increased C. concisus growth. An oral C. concisus strain was found to express a number of putative virulence proteins and the expression levels of these proteins were not affected by H2. The levels of H2 appeared to affect the optimal growth of C. concisus. This study provides useful information in understanding the natural colonization site and pathogenicity of C. concisus. |
url |
http://dx.doi.org/10.1155/2014/476047 |
work_keys_str_mv |
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