Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats
Development of obesity-associated comorbidities is related to chronic inflammation, which has been linked to gut microbiota dysbiosis. Thus, modulating gut microbiota composition could have positive effects for metabolic disorders, supporting the use of probiotics as potential therapeutics in vivo,...
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doaj-5ec3fd300e9d4317b13eff41ed93c8692020-11-24T21:48:59ZengMDPI AGNutrients2072-66432019-08-01119197510.3390/nu11091975nu11091975Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed RatsSunhye Lee0Rebecca Kirkland1Zachary I. Grunewald2Qingshen Sun3Louise Wicker4Claire B. de La Serre5Department of Anatomy, Physiology, and Cell Biology, University of California Davis School of Veterinary Medicine, Davis, CA 95616, USADepartment of Foods and Nutrition, University of Georgia, Athens, GA 30602, USADepartment of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65211, USACollege of Life Science, Heilongjiang University, Harbin 150080, ChinaSchool of Nutrition and Food Sciences, Louisiana State University AgCenter, 101 LSU Union Square, Baton Rouge, LA 70803, USADepartment of Foods and Nutrition, University of Georgia, Athens, GA 30602, USADevelopment of obesity-associated comorbidities is related to chronic inflammation, which has been linked to gut microbiota dysbiosis. Thus, modulating gut microbiota composition could have positive effects for metabolic disorders, supporting the use of probiotics as potential therapeutics in vivo, which may be enhanced by a microencapsulation technique. Here we investigated the effects of non-encapsulated or pectin-encapsulated probiotic supplementation (<i>Lactobacillus paracasei subsp. paracasei</i> L. casei W8<sup>®</sup>; L. casei W8) on gut microbiota composition and metabolic profile in high-fat (HF) diet-fed rats. Four male Wistar rat groups (<i>n</i> = 8/group) were fed 10% low-fat, 45% HF, or HF with non-encapsulated or encapsulated L. casei W8 (4 × 10<sup>7</sup> CFU/g diet) diet for seven weeks. Microbiota composition, intestinal integrity, inflammatory profiles, and glucose tolerance were assessed. Non-encapsulated and pectin-encapsulated probiotic supplementation positively modulated gut microbiota composition in HF-fed male rats. These changes were associated with improvements in gut barrier functions and local and systemic inflammation by non-encapsulated probiotics and improvement in glucose tolerance by encapsulated probiotic treatment. Thus, these findings suggest the potential of using oral non-encapsulated or encapsulated probiotic supplementation to ameliorate obesity-associated metabolic abnormalities.https://www.mdpi.com/2072-6643/11/9/1975probioticsmicroencapsulationgut microbiotaintestinal epithelial barrierinflammationglucose tolerance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sunhye Lee Rebecca Kirkland Zachary I. Grunewald Qingshen Sun Louise Wicker Claire B. de La Serre |
spellingShingle |
Sunhye Lee Rebecca Kirkland Zachary I. Grunewald Qingshen Sun Louise Wicker Claire B. de La Serre Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats Nutrients probiotics microencapsulation gut microbiota intestinal epithelial barrier inflammation glucose tolerance |
author_facet |
Sunhye Lee Rebecca Kirkland Zachary I. Grunewald Qingshen Sun Louise Wicker Claire B. de La Serre |
author_sort |
Sunhye Lee |
title |
Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats |
title_short |
Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats |
title_full |
Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats |
title_fullStr |
Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats |
title_full_unstemmed |
Beneficial Effects of Non-Encapsulated or Encapsulated Probiotic Supplementation on Microbiota Composition, Intestinal Barrier Functions, Inflammatory Profiles, and Glucose Tolerance in High Fat Fed Rats |
title_sort |
beneficial effects of non-encapsulated or encapsulated probiotic supplementation on microbiota composition, intestinal barrier functions, inflammatory profiles, and glucose tolerance in high fat fed rats |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-08-01 |
description |
Development of obesity-associated comorbidities is related to chronic inflammation, which has been linked to gut microbiota dysbiosis. Thus, modulating gut microbiota composition could have positive effects for metabolic disorders, supporting the use of probiotics as potential therapeutics in vivo, which may be enhanced by a microencapsulation technique. Here we investigated the effects of non-encapsulated or pectin-encapsulated probiotic supplementation (<i>Lactobacillus paracasei subsp. paracasei</i> L. casei W8<sup>®</sup>; L. casei W8) on gut microbiota composition and metabolic profile in high-fat (HF) diet-fed rats. Four male Wistar rat groups (<i>n</i> = 8/group) were fed 10% low-fat, 45% HF, or HF with non-encapsulated or encapsulated L. casei W8 (4 × 10<sup>7</sup> CFU/g diet) diet for seven weeks. Microbiota composition, intestinal integrity, inflammatory profiles, and glucose tolerance were assessed. Non-encapsulated and pectin-encapsulated probiotic supplementation positively modulated gut microbiota composition in HF-fed male rats. These changes were associated with improvements in gut barrier functions and local and systemic inflammation by non-encapsulated probiotics and improvement in glucose tolerance by encapsulated probiotic treatment. Thus, these findings suggest the potential of using oral non-encapsulated or encapsulated probiotic supplementation to ameliorate obesity-associated metabolic abnormalities. |
topic |
probiotics microencapsulation gut microbiota intestinal epithelial barrier inflammation glucose tolerance |
url |
https://www.mdpi.com/2072-6643/11/9/1975 |
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