Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone
Progressive muscle loss with aging results in decreased physical function, frailty, and impaired metabolic health. Deficits in anabolic signaling contribute to an impaired ability for aged skeletal muscle to adapt in response to exercise and protein feeding. One potential contributing mechanism coul...
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doaj-5ed325ef4cfd497bbe0d85797a0fd7992020-11-25T00:23:33ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2019-06-01610.3389/fnut.2019.00091453472Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise AloneRandall F. D'Souza0Randall F. D'Souza1Nina Zeng2James F. Markworth3James F. Markworth4Vandre C. Figueiredo5Vandre C. Figueiredo6Christopher P. Hedges7Aaron C. Petersen8Paul A. Della Gatta9David Cameron-Smith10David Cameron-Smith11David Cameron-Smith12Cameron J. Mitchell13Cameron J. Mitchell14Liggins Institute, The University of Auckland, Auckland, New ZealandDiscipline of Nutrition, The University of Auckland, Auckland, New ZealandLiggins Institute, The University of Auckland, Auckland, New ZealandLiggins Institute, The University of Auckland, Auckland, New ZealandDepartment of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, United StatesLiggins Institute, The University of Auckland, Auckland, New ZealandCollege of Health Sciences, University of Kentucky, Lexington, KY, United StatesApplied Surgery and Metabolism Laboratory, School of Biological Sciences, University of Auckland, Auckland, New ZealandInstitute for Health and Sport (iHeS), Victoria University, Melbourne, VIC, AustraliaInstitute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, VIC, AustraliaLiggins Institute, The University of Auckland, Auckland, New ZealandFood and Bio-based Products, AgResearch Grasslands, Palmerston North, New ZealandThe Riddet Institute, Massey University, Palmerston North, New ZealandLiggins Institute, The University of Auckland, Auckland, New Zealand0School of Kinesiology, University of British Colombia, Vancouver, BC, CanadaProgressive muscle loss with aging results in decreased physical function, frailty, and impaired metabolic health. Deficits in anabolic signaling contribute to an impaired ability for aged skeletal muscle to adapt in response to exercise and protein feeding. One potential contributing mechanism could be exerted by dysregulation of microRNAs (miRNAs). Therefore, the aim of this study was to determine if graded protein doses consumed after resistance exercise altered muscle miRNA expression in elderly men. Twenty-three senior men (67.9 ± 0.9 years) performed a bout of resistance exercise and were randomized to consume either a placebo, 20 or 40 g of whey protein (n = 8, n = 7, and n = 8, respectively). Vastus lateralis biopsies were collected before, 2 and 4 h after exercise. Expression of 19 miRNAs, previously identified to influence muscle phenotype, were measured via RT-PCR. Of these, miR-16-5p was altered with exercise in all groups (p = 0.032). Expression of miR-15a and-499a increased only in the placebo group 4 h after exercise and miR-451a expression increased following exercise only in the 40 g whey supplementation group. Changes in p-P70S6KThr389 and p-AktSer473 following exercise were correlated with alterations in miR-208a and-499a and-206 expression, irrespective of protein dose, suggesting a possible role for miRNA in the regulation of acute phosphorylation events during early hours of exercise recovery.https://www.frontiersin.org/article/10.3389/fnut.2019.00091/fullskeletal musclemTOR pathwaymicroRNAolder adultsresistance trainingP70S6 K |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Randall F. D'Souza Randall F. D'Souza Nina Zeng James F. Markworth James F. Markworth Vandre C. Figueiredo Vandre C. Figueiredo Christopher P. Hedges Aaron C. Petersen Paul A. Della Gatta David Cameron-Smith David Cameron-Smith David Cameron-Smith Cameron J. Mitchell Cameron J. Mitchell |
spellingShingle |
Randall F. D'Souza Randall F. D'Souza Nina Zeng James F. Markworth James F. Markworth Vandre C. Figueiredo Vandre C. Figueiredo Christopher P. Hedges Aaron C. Petersen Paul A. Della Gatta David Cameron-Smith David Cameron-Smith David Cameron-Smith Cameron J. Mitchell Cameron J. Mitchell Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone Frontiers in Nutrition skeletal muscle mTOR pathway microRNA older adults resistance training P70S6 K |
author_facet |
Randall F. D'Souza Randall F. D'Souza Nina Zeng James F. Markworth James F. Markworth Vandre C. Figueiredo Vandre C. Figueiredo Christopher P. Hedges Aaron C. Petersen Paul A. Della Gatta David Cameron-Smith David Cameron-Smith David Cameron-Smith Cameron J. Mitchell Cameron J. Mitchell |
author_sort |
Randall F. D'Souza |
title |
Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone |
title_short |
Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone |
title_full |
Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone |
title_fullStr |
Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone |
title_full_unstemmed |
Whey Protein Supplementation Post Resistance Exercise in Elderly Men Induces Changes in Muscle miRNA's Compared to Resistance Exercise Alone |
title_sort |
whey protein supplementation post resistance exercise in elderly men induces changes in muscle mirna's compared to resistance exercise alone |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Nutrition |
issn |
2296-861X |
publishDate |
2019-06-01 |
description |
Progressive muscle loss with aging results in decreased physical function, frailty, and impaired metabolic health. Deficits in anabolic signaling contribute to an impaired ability for aged skeletal muscle to adapt in response to exercise and protein feeding. One potential contributing mechanism could be exerted by dysregulation of microRNAs (miRNAs). Therefore, the aim of this study was to determine if graded protein doses consumed after resistance exercise altered muscle miRNA expression in elderly men. Twenty-three senior men (67.9 ± 0.9 years) performed a bout of resistance exercise and were randomized to consume either a placebo, 20 or 40 g of whey protein (n = 8, n = 7, and n = 8, respectively). Vastus lateralis biopsies were collected before, 2 and 4 h after exercise. Expression of 19 miRNAs, previously identified to influence muscle phenotype, were measured via RT-PCR. Of these, miR-16-5p was altered with exercise in all groups (p = 0.032). Expression of miR-15a and-499a increased only in the placebo group 4 h after exercise and miR-451a expression increased following exercise only in the 40 g whey supplementation group. Changes in p-P70S6KThr389 and p-AktSer473 following exercise were correlated with alterations in miR-208a and-499a and-206 expression, irrespective of protein dose, suggesting a possible role for miRNA in the regulation of acute phosphorylation events during early hours of exercise recovery. |
topic |
skeletal muscle mTOR pathway microRNA older adults resistance training P70S6 K |
url |
https://www.frontiersin.org/article/10.3389/fnut.2019.00091/full |
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